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Comprehensive analysis of the factor in connection with ureteric bud branching of the initial metanephros of mouse embryos

Research Project

Project/Area Number 17590179
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionFujita Health University

Principal Investigator

HASEGAWA Yoshimi  Fujita Health University, School of Medicine, Anatomy, Research Assistant (40288494)

Co-Investigator(Kenkyū-buntansha) NOMURA Ryuji  Fujita Health University, School of Medicine, Anatomy, Assistant Professor (20325161)
SHIMOMURA Atsushi  Fujita Health University, School of Medicine, Anatomy, Research Assistant (50340237)
KOGO Akiko  Fujita Health University, School of Medicine, Anatomy, Research Assistant (20340242)
YAMADA Koji  Fujita Health University, School of Medicine, Anatomy, Assistant Professor (60278306)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,810,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsICAT / Wnt signaling pathway / ureteric bud branching / renal agenesis / 後腎欠損 / アポトーシス / 後腎発生
Research Abstract

The Wnt signals, which are critical for embryonic development in vertebrates, stabilize β-catenin and localize it in the nucleus. T cell factor/lymphoid enhancer factor (TCF/LEF) protein mediates the transcriptional regulation of Wnt/β-catenin target genes that play critical roles in cell growth, proliferation and differentiation. ICAT, inhibitor of β-catenin and T cell factor, is a negative regulator of the Wnt signaling pathway that interferes with the interaction between β-catenin and T cell factor. We used knockout mice of null mutation in the ICAT gene. ICAT-deficient (ICAT^<-/->) embryos exhibited malformation of the forebrain and craniofacial bones. Furthermore, renal agenesis was found in 13% of ICAT^<-/-> embryos. Some ICAT^<-/-> embryos exhibit unilateral or bilateral renal agenesis. In this study, we searched developmental processes in the ICAT^<-/-> kidney.
ICAT was highly expressed in both the ureteric bud (UB) and the surrounding metanephric mesenchymal (MM) cells in the m … More etanephros of embryonic day E11.5-E13.5 wild-type (ICAT^<+/+>) mouse. We next performed whole-mount immunostaining of E12.5 metanephros for cytokeratin to visualize UB branching. The number of UB tips in the metanephros was ICAT^<-/-> metanephroi, whereas in the ICAT^<+/-> metanephroi, with a significant difference. In the E12.5-ICAT^<-/-> metanephros, UB branching was delayed, and a T-shaped, bifurcated UB was frequently seen; this was never seen in the E12.5-ICAT^<+/-> metanephros. This indicates that the UB branching was disturbed and arrested at the T-shaped stage in some ICAT^<-/-> metanephroi. Next study, apoptotic cells in metanephroi were detected by a TUNEL assay around the T-shaped stage, in ICAT^<-/-> and ICAT^<+/+> embryos. More apoptotic MM cells were detected in the ICAT^<-/->metanephros than in the ICAT^<+/+> metanephros. These results suggest that the loss of ICAT gene function causes the arrest of UB branching and the apoptotic death of MM cells, resulting in renal agenesis. Less

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (13 results)

All 2008 2007 2005

All Journal Article (5 results) (of which Peer Reviewed: 3 results) Presentation (8 results)

  • [Journal Article] Loss of ICAT gene function leads to arrest of ureteric bud branching and renal agenesis2007

    • Author(s)
      Y.hasegawa, K.Satoh, A.Iizuka-Kogo, A.Shimomura, R.Nomura, T.Akiyama, T.Senda
    • Journal Title

      Biochemical and Biophysical Research Communications 362

      Pages: 988-994

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] マウス腎臓発生過程におけるICAT mRNAとICAT蛋白質の発現と局在2007

    • Author(s)
      長谷川 義美、向後 晶子、千田 隆夫
    • Journal Title

      藤田学園医学会誌 31(2)

      Pages: 115-119

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Loss of ICAT gene function leads to arrest of ureteric bud branching and renal agenesis2007

    • Author(s)
      Y. hasegawa, K. Satoh, A. Iizuka-Kogo, A. Shimomura, R. Nomura, T. Akiyama, T. Senda
    • Journal Title

      Biochemical and Biophysical Research Communications 362

      Pages: 988-994

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Journal Article] Expression and Localization of ICAT-mRNA and -Protein during Development of the Mouse Kidney.2007

    • Author(s)
      Y. hasegawa, A. Iizuka-Kogo, T. Senda
    • Journal Title

      Bulletin of The Fujita Medical Society 31(2)

      Pages: 115-119

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] マウス腎臓発生過程におけるICAT mRNAとICAT蛋白質の発現と局在2007

    • Author(s)
      長谷川義美、向後晶子、千田隆夫
    • Journal Title

      藤田学園医学会誌 31(2)

      Pages: 115-119

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Wntシグナル系抑制因子ICATノックアウトマウスににおける腎臓欠損のメカニズム2008

    • Author(s)
      千田 隆夫、長谷川 義美、向後 晶子、下村 敦司、野村 隆士
    • Organizer
      日本解剖学会第113回全国学術集会
    • Place of Presentation
      大分
    • Year and Date
      2008-03-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The mechanism of the renal agenesis by KO of the Wnt signaling pathway inhibitory factor ICAT2008

    • Author(s)
      T. Senda, Y. hasegawa, A. Iizuka-Kogo, A. Shimomura, R. Nomura
    • Organizer
      Japanese Association of Anatomists, 113th Chubu chapter Annual Meeting
    • Place of Presentation
      Oita
    • Year and Date
      2008-03-28
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Wntシグナル系抑制因子ICATノックアウトマウスにおける腎臓欠損のメカニズム2008

    • Author(s)
      千田隆夫、長谷川義美、向後晶子、下村敦司、野村隆士
    • Organizer
      第113回日本解剖学会総会・全国学術集会
    • Place of Presentation
      大分大学医学部
    • Year and Date
      2008-03-28
    • Related Report
      2007 Annual Research Report
  • [Presentation] Wntシグナル系抑制因子ICATのノックアウトによる腎臓欠損のメカニズム2007

    • Author(s)
      千田 隆夫、長谷川 義美、向後 晶子、下村 敦司、野村 隆士
    • Organizer
      日本解剖学会第67回中部支部学術集会
    • Place of Presentation
      長久手
    • Year and Date
      2007-10-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The mechanism of the renal agenesis by KO of the Wnt signaling pathway inhibitory factor ICAT2007

    • Author(s)
      T. Senda, Y. hasegawa, A. Iizuka-Kogo, A. Shimomura, R. Nomura
    • Organizer
      Japanese Association of Anatomists, 67th Chubu chapter Annual Meeting
    • Place of Presentation
      Nagakute
    • Year and Date
      2007-10-14
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Wntシグナル系抑制因子ICATのノックアウトによる腎臓欠損のメカニズム2007

    • Author(s)
      千田隆夫、長谷川義美、向後晶子、下村敦司、野村隆士
    • Organizer
      日本解剖学会第67回中部支部学術集会
    • Place of Presentation
      愛知医科大学医学部
    • Year and Date
      2007-10-13
    • Related Report
      2007 Annual Research Report
  • [Presentation] ICATノックアウトマウスにおける腎臓欠損のメカニズム2005

    • Author(s)
      長谷川 義美、向後 晶子、千田 隆夫
    • Organizer
      藤田学園医学会第37回総会
    • Place of Presentation
      豊明
    • Year and Date
      2005-10-06
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The mechanism of the renal agenesis in ICAT knockout mice2005

    • Author(s)
      Y. hasegawa, A. Iizuka-Kogo, T. Senda
    • Organizer
      Bulletin of the Fujita Medical Society of the 37th Annual Meeting
    • Place of Presentation
      Toyoake
    • Year and Date
      2005-10-06
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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