• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Research on a novel mechanism for anti-arrhythmogenic effects of electrical vagal nerve stimulation during acute myocardial ischemia

Research Project

Project/Area Number 17590187
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionOkayama University

Principal Investigator

ANDO Motonori  Okayama University, Faculty of Education, Associate Professor, 教育学部, 助教授 (20222789)

Co-Investigator(Kenkyū-buntansha) KATARE Rajesh G.  Kochi Medical School, Faculty of Medicine, Research Associate, 医学部, 助手 (20380313)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2005: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsvagal nerve / electrical stimulation / arrhythmia / gap junction / connexin / acetylcholine
Research Abstract

Myocardial ischemia (MI) leads to derangements in cellular electrical stability and the generation of lethal arrhythmias. Vagal nerve stimulation has been postulated to contribute to the antifibrillatory effect. In this study, we demonstrate a novel mechanism for the antiarrhythmogenic properties of electrical vagal nerve stimulation during acute MI. Under anesthesia, Wistar rats underwent 30 minutes of left coronary artery (LCA) ligation with vagal stimulation (MI-VS group) and with sham stimulation (MI-SS group). Eight of the 12 rats in the MI-SS group had ventricular tachyarrhythmia (VT) during 30-minute LCA ligation; on the other hand, VT occurred in only 1 of the 11 rats in the MI-VS group (67% versus 9%, respectively). Atropine administration abolished the antiarrhythmogenic effect of vagal stimulation. Immunoblotting revealed that the MI-SS group showed a marked reduction in the amount of phosphorylated connexin43 (Cx43), whereas the MI-VS group showed only a slight reduction compared with the sham operation and sham stimulation group (37+/-20% versus 79+/-18%). Immunohistochemistry confirmed that the MI-induced loss of Cx43 from intercellular junctions was prevented by vagal stimulation. In addition, studies with rat primary-cultured cardiomyocytes demonstrated that acetylcholine (ACh) effectively prevented the hypoxia-induced loss of phosphorylated Cx43 and ameliorated the loss of cell-to-cell communication as determined by Lucifer Yellow dye transfer assay, which supports the in vivo results. Furthermore, in-vitro studies also revealed that ACh induced Akt phosphorylation, which was inhibited by wortmannin. These results suggest that ACh protects cardiomyocytes through the PI3K/Akt pathway. In conclusion, vagal nerve stimulation exerts both anti-arrhythmogenic and anti-apoptotic effects during acute MI and thus plays a critical role in improving ischemia-induced electrical instability and in activating cell-survival signals.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (10 results)

All 2006 2005

All Journal Article (10 results)

  • [Journal Article] Acetylcholine inhibits the hypoxia-induced reduction of connexin43 protein in rat cardiomyocytes.2006

    • Author(s)
      Zhang Y
    • Journal Title

      Journal of Pharmacological Sciences 101

      Pages: 214-222

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Nitric oxide stimulates vascular endothelial growth factor production in cardiomyocytes involved in angiogenesis.2006

    • Author(s)
      Kuwabara M
    • Journal Title

      Journal of Physiological Sciences 56

      Pages: 95-101

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Artificial baroreflex : clinical application of a bionic baroreflex system.2006

    • Author(s)
      Yamasaki F
    • Journal Title

      Circulation 113

      Pages: 634-639

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Acetylcholine inhibits the hypoxia-induced reduction of connexin43 protein in rat cardiomyocytes.2006

    • Author(s)
      Zhang Y
    • Journal Title

      Journal of Pharmacological. Sciences 101

      Pages: 214-222

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Efferent vagal nerve stimulation protects heart against ischemia-induced arrhythmias by preserving connexin43 protein.2005

    • Author(s)
      Ando M
    • Journal Title

      Circulation 112

      Pages: 164-170

    • NAID

      120001794313

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Acetylcholine from vagal stimulation protects cardiomyocytes against ischemia and hypoxia involving additive non-hypoxic induction of HIF-lα.2005

    • Author(s)
      Kakinuma Y
    • Journal Title

      FEBS Letters 579

      Pages: 54-61

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Dielectric behavior of pulmonary edema induced in the rat lung.2005

    • Author(s)
      Yamashiro T
    • Journal Title

      Respiratory Physiology & Neurobiology 145

      Pages: 91-100

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Acetylcholine from vagal stimulation protects cardiomyocytes against ischemia and hypoxia involving additive non-hypoxic induction of HIF-la.2005

    • Author(s)
      Kakinuma Y
    • Journal Title

      FEBS Letters 579

      Pages: 54-61

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Acetylcholine from vagal stimulation protects cardiomyocytes against ischemia and hypoxia involving additive non-hypoxic induction of HIF-1α.2005

    • Author(s)
      Kakinuma Y
    • Journal Title

      FEBS Letters 579

      Pages: 54-61

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Dielectric behavior of pulmonary edema induced in the rat lung.2005

    • Author(s)
      Yamashiro T
    • Journal Title

      Respiratory Physiology & Neurobiology 145

      Pages: 91-100

    • Related Report
      2005 Annual Research Report

URL: 

Published: 2005-04-01   Modified: 2021-04-07  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi