• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Investigation on atrial natriuretic peptide as an endogenous antipyretic

Research Project

Project/Area Number 17590204
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental physiology (including Physical medicine and Nutritional physiology)
Research InstitutionTottori University

Principal Investigator

WATANABE Tatsuo  Tottori University, Faculty of Medicine, Professor, 医学部, 教授 (60182929)

Co-Investigator(Kenkyū-buntansha) IMOTO Toshiaki  Tottori University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (10109639)
MIYOSHI Michio  Tottori University, Faculty of Medicine, Research Associate, 医学部, 助手 (20093627)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2005: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsANP / LPS / IL-1 / fever / rat / NF-κB / AP-1 / microglial cell / NO / 形態変化 / 脾臓 / 脳 / アンギオテンシンII
Research Abstract

We investigated whether natriuretic peptide (NP) acts as an endogenous antipyretic inside and/or outside the blood-brain barrier in rats made febrile by intravenous (i.v.) injection of bacterial endotoxin (lipopolysaccharide ; LPS). LPS induced a triphasic fever, the second phase of which was significantly enhanced by an i.v. injection of the NP-receptor antagonist HS-142-1. In contrast., the same antagonist (i.v.) had no effect on the fever induced by i.v. injection of interleukin-β (IL-1β). An i.v. administration of HS-142-1 enhanced the LPS (i.v.)-induced IL-1β response in the rat spleen. An i.v. treatment with atrial NP (ANP) significantly attenuated the second phase of the LPS-induced fever. Intracerebroventricular (i.c.v.) injection of the above NP-receptor antagonist resulted in an augmentation of the third phase of the fever induced by i.v. administration of LPS, the same phase that, was attenuated by ANP given i.c.v. When given i.c.v., the antagonist had no effect on the fever … More induced by i.v. IL-1β. On the other hand, we used primary cultures of rat brain macrophage-like cells (i.e., microglial cells) to investigate whether ANP binding to its receptors inhibits LPS-induced microglial activation via effects on the activation of the proinflammatory transcription factors NF-κB and AP-1. UPS-stimulated microglial cells showed increases in nitrite and IL-1 concentrations, and in the expression of IL-1 mRNA, as well as a morphological change from an amoeboid shape to a multipolar rod shape. These effects were all significantly inhibited by treatment with ANP. The inhibition by ANP of the LPS-induced nitrite response was abrogated by HS-142-1. NF-κB and AP-1 activities were enhanced in LPS-stimulated microglial cells, and these enhancements were significantly suppressed by ANP. Collectively, these results suggest that the production of pyrogenic cytokines may be inhibited by NP acting inside and outside the blood-brain barrier, leading to an inhibition of the fever in rats. Less

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (19 results)

All 2006 2005 Other

All Journal Article (19 results)

  • [Journal Article] Effect of natriuretic peptide receptor antagonist on lipopolysaccharide-induced fever in rats. Is natriuretic peptide an endogenous antipyretic?2006

    • Author(s)
      Miyoshi, M.
    • Journal Title

      Journal of Pharmacology and Experimental Therapeutics 318

      Pages: 1163-1170

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] ANP inhibits LPS-induced stimulation of rat microglial cells by suppressing NF-κB and AP-1 activations.2006

    • Author(s)
      Moriyama, N.
    • Journal Title

      Biochemical and Biophysical Research Communications 350(2)

      Pages: 322-328

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of central injection of angiotensin-converting-enzyme inhibitor and angiotensin type 1 receptor antagonist on the brain NF-κB and AP-1 activities of rats given LPS.2006

    • Author(s)
      Watanabe, K.
    • Journal Title

      Peptides 27(6)

      Pages: 1538-1546

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Systemic administration of polymyxin B induces hypothermia in rats via an inhibitory effect on metabolic rate.2006

    • Author(s)
      Moriyama, N.
    • Journal Title

      European Journal of Pharmacology 541(1-2)

      Pages: 38-43

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effect of natriuretic peptide receptor antagonist on lipopolysaccharide-induced fever in rats. Is natriuretic peptide an endogenous antipyretic?2006

    • Author(s)
      Miyoshi, M., Kitagawa, Y., Imoto, T., Watanabe, T.
    • Journal Title

      Journal of Pharmacology and Experimental Therapeutics 318

      Pages: 1163-1170

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] ANP inhibits LPS-induced stimulation of rat microglial cells by suppressing NF-κB and AP-1 activations.2006

    • Author(s)
      Moriyama, N., Taniguchi, M., Miyano, K., Miyoshi, M., Watanabe, T.
    • Journal Title

      Biochemical and Biophysical Research Communications 350(2)

      Pages: 322-328

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of central injection of angiotensin-converting-enzyme inhibitor and angiotensin type 1 receptor antagonist on the brain NF-κB and AP-1 activities of rats given LPS.2006

    • Author(s)
      Watanabe, K., Taniguchi, M., Miyoshi, M., Shimizu, H., Imoto, T., Sato, K., Watanabe, T.
    • Journal Title

      Peptides 27(6)

      Pages: 1538-1546

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Systemic administration of polymyxin B induces hypothermia in rats via an inhibitory effect on metabolic rate.2006

    • Author(s)
      Moriyama, N., Miyoshi, M., Imoto, T., Maruyama, M., Shido, O., Watanabe, T.
    • Journal Title

      European Journal of Pharmacology 541(1-2)

      Pages: 38-43

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effect of natriuretic peptide receptor antagonist on lipopolysaccharide-induced fever in lats. Is natriuretic peptied an endogenous antipyretic?2006

    • Author(s)
      Miyoshi, M., et al.
    • Journal Title

      Journal of Pharmacology and Experimental Therapeutics 318

      Pages: 1163-1170

    • Related Report
      2006 Annual Research Report
  • [Journal Article] ANP inhibits LPS-induced stimulation of rat microglial cells by suppressing NF_kB and AP-1 activations.2006

    • Author(s)
      Moriyama, N., et al.
    • Journal Title

      Biochemical and Biophysical Research Communications 350(2)

      Pages: 322-328

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Effects of centra injection of angiotensin-converting-enzyme inhibitor and angiotensin type 1 receptor antagonist on the brain NF-_kB and AP-1 activities of rats given LPS.2006

    • Author(s)
      Watanabe, K., et al.
    • Journal Title

      Peptides 27(6)

      Pages: 1538-1546

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Systemic administration of polymyxin B induces hypothermia in rats via an inhibitory effect on metabolic rats.2006

    • Author(s)
      Moriyama, N., et al.
    • Journal Title

      European Journal of Pharmacology 541(1-2)

      Pages: 38-43

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Are transcription factors NF-κB and AP-1 involved in the ANG II-stimulated production of proinflammatory cytokines induced by LPS in dehydrated rats?2005

    • Author(s)
      Sasaki, K.
    • Journal Title

      American Journal of Physiology 289(6)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Systemic administration of lipopolysaccharide upregulates angiotensin II expression in rat renal tubules : immunohistochemical and ELISA studies.2005

    • Author(s)
      Fukada, M.
    • Journal Title

      Peptides 26(11)

      Pages: 2215-2221

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Are transcription factors NF-κB and AP-1 involved in the ANG II-stimulated production of proinflammatory cytokines induced by LPS in dehydrated rats?2005

    • Author(s)
      Sasaki, K., Taniguchi, M., Miyoshi, M., Goto, O., Sato, K., Watanabe, T.
    • Journal Title

      American Journal of Physiology 289(6)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Systemic administration of lipopolysaccharide upregulates angiotensin II expression in rat renal tubules : immunohistochemical and ELISA studies.2005

    • Author(s)
      Fukada, M., Kato, S., Miyoshi, M..Yamaguchi, K., Imoto, T., Watanabe, T.
    • Journal Title

      Peptides 26(11)

      Pages: 2215-2221

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Systemic administration of lipopolysaccharide upregulates angiotensin II expression in rat renal tubules : immunohistochemical and ELISA studies.2005

    • Author(s)
      Fukada, M., et al.
    • Journal Title

      Peptides 26(11)

      Pages: 2215-2221

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Are transcription factors NF-κB and AP-1 involved in the ANG II-stimulated production of proinflammatory cytokines induced by LPS in dehydrated rats?2005

    • Author(s)
      Sasaki, K., et al.
    • Journal Title

      American Journal of Physiology 289(6)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Effects of central injection of angiotensin-converting-enzyme inhibitor and angiotensin type 1 receptor antagonist on the brain NF-κB and AP-1 activities of rats given LPS.

    • Author(s)
      Watanabe, K., et al.
    • Journal Title

      Peptides (in the press)

    • Related Report
      2005 Annual Research Report

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi