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An exhaustive search of new bioactive peptides based on prediction by bioinformatics using human genome structures

Research Project

Project/Area Number 17590214
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General pharmacology
Research InstitutionChiba University

Principal Investigator

KIMURA Sadao  Chiba University, Graduate School of Medicine, 大学院医学研究院, 教授 (40134225)

Co-Investigator(Kenkyū-buntansha) KASUYA Yoshitoshi  Chiba University, Graduate School of Medicine, Associate Professor, 大学院医学研究院, 助教授 (70221877)
NISHIYAMA Mariko  Chiba University, Graduate School of Medicine, Research Associate, 大学院医学研究院, 助手 (00092081)
MOROI Kayoko  Chiba University, Graduate School of Medicine, Research Associate, 大学院医学研究院, 助手 (80110352)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2005: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsGPCR / orphan GPCR / bioactive peptide / genome structure / bioinformatics / バイオインフォーマティクス
Research Abstract

1) Based on biosynthetic rules of known peptide hormones, we tried to predict candidate bioactive peptides from human genome structure as peptide ligands for orphan G protein-coupled receptors (GPCRs).
2) We developed a program to predict bioactive peptide ligands and predicted 1750 candidate peptides for GPCRs and synthesized them by chemical synthesis.
3) To find out new ligands for orphan GPCRs, we measured bioactivity of these synthetic peptides using 26 kinds of cells for detecting changes in their intracellular calcium concentration induced by these peptides.
4) We detected 140 peptides, which had activity. However, it was found that all these peptides are active on cells only in a range of high concentrations (more than 10^<-6> M).
5) It seemed that candidate peptides crossed-acted on orphan GPCRs and produced a intracellular calcium signal, and it was estimated that these were not true GPCR ligands because known bioactive peptides are active in a concentration range of 10^<-9> -10^<-8> M.
6) Further improvement of program software to predict candidate peptides from genome structures is needed, and it seems that development of new screening methods to detect bioactive peptides effectively, in addition to the intracellular calcium assay, is essential.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (8 results)

All 2006 2005

All Journal Article (8 results)

  • [Journal Article] Involvement of p38alpha mitgen-activayed protein kinase in lung metastasis of tumor cells.2006

    • Author(s)
      Matsuo Y, Amano S, Furuya M, Namiki K, Sakurai K, Nishiyama M, Sudo T, Tatsumi K, Kuriyama T, Kimura S, Kasuya Y
    • Journal Title

      J. Biol. Chem. 281・48

      Pages: 36767-36775

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Involvement of p38alpha mitogen-activated protein kinase in lung metastasis of tumor cells.2006

    • Author(s)
      Matsuo Y, Amano S, Furuya M, Namiki K, Sakurai K, Nishiyama M, Sudo T, Tatsumi K, Kuriyama T, Kimura S, Kasuya Y.
    • Journal Title

      J. Biol. Chem. 281

      Pages: 36767-36775

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Pathophysiology of Tumor Neovascularization.2005

    • Author(s)
      Furuya M, Nishiyama M, Kasuya Y, Kimura S, Ishikura H
    • Journal Title

      Vasc. Health Risk Management 1・4

      Pages: 277-290

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] RGS4 and RGS5 are in vivo substrates of the N-end rule pathway.2005

    • Author(s)
      Lee MJ, Tasaki T, Moroi K, An JY, Kimura S, Davydov IV, Kwon YT
    • Journal Title

      Proc. Natl. Acad. Sci. USA 102・42

      Pages: 15030-15035

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Pathophysiology of Tumor Neovascularization.2005

    • Author(s)
      Furuya M, Nishiyama M, Kasuya Y, Kimura S, Ishikura H
    • Journal Title

      Vasc. Health Risk Management I(4)

      Pages: 277-290

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] RGS4 and RGS5 are in vivo substrates of the N-end rule pathway.2005

    • Author(s)
      Lee MJ, Tasaki T, Moroi K, An JY, Kimura S, Davydov IV, Kwon YT
    • Journal Title

      Proc. Natl. Acad. Sci. USA 102

      Pages: 15030-15035

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Pathophysiology of Tumor Neovascularization.2005

    • Author(s)
      Furuya M, Nishiyama M, Kasuya Y, Kimura S, Ishikura H
    • Journal Title

      Vasc.Health Risk Management 1・4

      Pages: 277-290

    • Related Report
      2005 Annual Research Report
  • [Journal Article] RGS4 and RGS5 are in vivo substrates of the N-end rule pathway.2005

    • Author(s)
      Lee MJ, Tasaki T, Moroi K, An JY, Kimura S, Davydov IV, Kwon YT
    • Journal Title

      Proc.Natl.Acad.Sci.USA 102・42

      Pages: 15030-15035

    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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