Research Project
Grant-in-Aid for Scientific Research (C)
It has been reported that overexpression in the culture cells of endoplasmic reticulum (ER) membrane proteins, such as microsomal aldehyde dehydrogenase (ALDH), causes the induction of a specific ER membrane structure (crystalloid ER, cER). We recently found for the first time that overexpression of an ER-unrelated, mitochondrial precursor for serine : pyruvate aminotransferase (SPT45K) induced the generation of cER, and overexpressed pOTC and pAd, other mitochondrial precursor proteins, also induced the ER aggregates. We also found that the chimeric fluorescent protein having the C terminal ER targeting sequence of ALDH at the C terminus had the remarkable ER aggregating activity by the overexpression. Because these cER (or ER aggregates) inducing activities were not observed in the cases of proteins (SPT43K, ALDHdC, EYFP) lacking organelle targeting signals, the activities is dependent on their structures. It was further indicated that the cER (ER aggregates) inducing activities was dependent on their expression levels in the cell. These data suggest that the cell has a novel system for the detection of the quality (structure) and the quantity of proteins expressed in the cytoplasm by inducing the new ER membrane structure.
All 2007 2006 2005 Other
All Journal Article (34 results)
Am. J. Pathol. (In press)
Am. J. Pathol. (in press)
Cancer Sci 97
Pages: 127-132
10020388517
Neuromol. Med. 8
Pages: 361-374
Cancer Res 66
Pages: 11623-11631
Pages: 8477-8483
Kidney Int 69
Pages: 1733-1740
FEBS Letters 560
Pages: 1753-1758
Biochem Biophys Res Commun 339
Pages: 367-374
Biochem Biophys Res Commun 340
Pages: 54-61
H. J. Med. Sci.(北海道医誌). 81
Pages: 101-109
10029919292
Cancer Sci. 97
Cancer Res. 66
Kidney Int. 69
Biochem Biophys Res Commun. 339
H.J.Med.Sci(北海道医誌). 81
H. J. Med. Sci(北海道医誌). 81
Biochem Biophys Res Commun. 340
FEBS Letters 579
Pages: 2557-2563
EMBO J. 24
Pages: 160-169
Kidney Int. (in press)
