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Molecular mechanism of the regulating factors for sarcoplasmic reticulum Ca-ATPase

Research Project

Project/Area Number 17590249
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionOsaka University

Principal Investigator

ASAHI Michio  Osaka University, Graduate School of Medicine, Assistant, 医学系研究科, 助手 (10397614)

Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2005: ¥2,900,000 (Direct Cost: ¥2,900,000)
KeywordsProteins / Cardiovascular Medicine, hypertention / Stress
Research Abstract

To examine the regulatory mechanism of cardiac type sarco(endo)plasmic reticulum Ca-ATPase(SERCA2a), which plays an important role in cardiac function, the primary cell culture of rat adult cardiomyocytes was espablished, and the physical profile and calcium transient of cardiomyocytes by electrical twitches were measured after various treatments. The research results are as follows. We investigated the effect of acrolein, an adduct of cigarette smoking, on cardiomyocytes. As a result, acrolein reduced the fuction of cardiomyocytes and eventually induced apoptosis. The apoptosis by acrolein was also induced in human umbilical vein endothelial cells (HUVEC), which might be involved in atherosclerosis related to cigarette smoking. The regulatory mechanism of cardiac ryanodine receptor, which is also the potent regulator of cardiac muscle, was examined. We found presenalin-2 as a regulator of cardiac ryanodine receptor. It was shown that presenalin-2 bound to cardiac ryanodine receptor in a calcium dependent manner and controlled the cardiac function. Furthermore, we revealed that phospholamban (PLN), an endogenous regulator of SERCA2a, was O-G1cNAcylated and the inhibitory function of PLN was regulated by the O-G1cNAcylation.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (14 results)

All 2007 2006 2005 Other

All Journal Article (14 results)

  • [Journal Article] Progression of heart failure was suppressed by inhibition of apoptosis signal-regulating kinasel(ASK1) via transcoronary gene transfer.2007

    • Author(s)
      Shungo Hikoso et al.
    • Journal Title

      J Am Coll Cardiol. (In press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The role of autophagy in cardiomyocytes in the basal state and in response to hemodynamic stress.2007

    • Author(s)
      Atsuko Nakai et al.
    • Journal Title

      Nat Med. (In press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The Asn^<418>-linked N-glycan of ErbB3 plays a crucial role in preventing spontaneous heterodimerization and tumor promotion.2007

    • Author(s)
      Shunichi Yokoe, Motoko Takahashi, Michio Asahi, Seung Ho Lee, Wei Li, Daisuke Osumi, Eiji Miyoshi, Naoyuki Taniguchi
    • Journal Title

      Cancer Research (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Acrolein produces nitric oxide through the elevation of intracellular calcium levels to induce apoptosis in human umbilical vein endothelial cells : Implications for smoke angiopathy.2006

    • Author(s)
      Yoshiko Misonou
    • Journal Title

      Nitric Oxide 14(2)

      Pages: 180-187

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Acrolein produces nitric oxide through the elevation of intracellular calcium levels to induce apoptosis in human umbilical vein endothelial cells : Implications for smoke angiopathy.2006

    • Author(s)
      Yoshiko Misonou et al.
    • Journal Title

      Nitric Oxide 14 (2)

      Pages: 180-187

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 活性酸素種のアポトーシスにおける役割2006

    • Author(s)
      朝日通雄, 朴用用軾, 谷口直之
    • Journal Title

      酸化ストレス-フリーラジカル医学生物学の最前線 Ver.2

      Pages: 111-115

    • Related Report
      2006 Annual Research Report
  • [Journal Article] スーパーオキシドジスムターゼとグルタチオンペルオキシダーゼ2006

    • Author(s)
      朝日通雄, 谷口直之
    • Journal Title

      酸化ストレスと心血管疾患-分子機構の解明から治療への応用まで Vol.218(1)

      Pages: 19-23

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Acrolein induces Hsp72 via both PKCdelta/JNK and calcium signaling pathways in human umbilical vein endothelial cells.2005

    • Author(s)
      Yoshiko Misonou
    • Journal Title

      Free Radic Res. 39(5)

      Pages: 507-512

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Presenilin 2 regulates the systolic function of heart by modulating Ca2+ signaling.2005

    • Author(s)
      Toshihiro Takeda
    • Journal Title

      FASEB J. 19(14)

      Pages: 2069-2071

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Acrolein induces Hsp72 via both PKCdelta/JNK and calcium signaling pathways in human umbilical vein endothelial cells.2005

    • Author(s)
      Yoshiko Misonou et al.
    • Journal Title

      Free Radic Res. 39 (5)

      Pages: 507-512

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Presenilin 2 regulates the systolic function of heart by modulating Ca2+ signaling.2005

    • Author(s)
      Toshihiro Takeda et al.
    • Journal Title

      FASEB J. 19 (14)

      Pages: 2069-2071

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Presenilin 2 regulates the systolic function of heart by modulating Ca2+ signaling2005

    • Author(s)
      Toshihiro Takeda
    • Journal Title

      FASEB J. 19(4)

      Pages: 2069-2071

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Progression of heart failure was suppressed by inhibition of apoptosis signal-regulating kinasel (ASK1) via transcoronary gene transfer.

    • Author(s)
      Shungo Hikoso
    • Journal Title

      J Am Coll Cardiol. (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The role of autophagy in cardiomyocytes in the basal state and in response to hemodynamic stress.

    • Author(s)
      Atsuko Nakai
    • Journal Title

      Nat Med. (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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