Analysis of Signal Transduction Pathway of Syk in Breast Cancer Cells-as a comparison with that of Her2

• Project/Area Number: 17590261
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Asahikawa Medical College
• Principal Investigator: TANIGUCHI Takanobu Asahikawa Medical College, Medicine, Professor, 医学部, 教授 (60217130)
• Co-Investigator(Kenkyū-buntansha): KATOH Tsuyoshi Asahikawa Medical College, Medicine, Associate Professor, 医学部, 助教授 (60194833) ; ISHIDA Atsuhiko Asahikawa Medical College, Medicine, Assistant Professor, 医学部, 助手 (90212886) ; TAKEUCHI Masayuki Asahikawa Medical College, Medicine, Assistant Professor, 医学部, 助手 (40226999)
• Project Period (FY): 2005 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥3,100,000 (Direct Cost: ¥3,100,000); Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000); Fiscal Year 2005: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: cancer / breast cancer / protein-tyrosine kinase / syk / Her2

Research Abstract

Breast cancer is one of the most popular cancers in women. Protein-tyrosine kinases have been shown to be involved in the pathogenesis of breast cancer. Her2 is a protein-tyrosine kinase which is believed to have oncogenic function, whereas Syk is another protein-tyrosine kinase which displays oncostatic potential. We have attempted to compare these two kinases focusing on their expression and their down stream signaling system. We estimated the expression of these kinases using immunoblotting in human breast cancer cell lines. In some of these cell lines, one breast cancer cell line, MDAMB453 expressed Her2 but no Syk. When Syk is introduced in MDAMB453, the expression of Her2 declined according to the expression level of Syk. The growth rate of Syk-introduced MDAMB453 clones was decreased as compared to that of mock cells. In MDAMB453 clones, administration of adriamycin induced an increase in Syk expression, which was correlated with a degradation of polyADP-ribose polymerase, a substrate of caspases. These data imply a mechanism of the opposite functions of these kinases in breast cancer carcionogenesis, in which Syk enhances apoptosis upon an exposure to cancer therapeutics.

Research Products

• [Journal Article] Recent advances in technologies to analyze protein kinases. (2006)
  • Author(s): Ishida, A, Kameshita, I., Sueyoshi, N., Taniguchi, T., Shigeri Y.
  • Journal Title: J Pharmacol Sci. (in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Characteristics of acid extrusion from Chinese hamster ovary cells expressed different prostaglandin EP receptors. (2006)
  • Author(s): Okada, Y., Taniguchi T, Morishima, S., Suzuki, F., Akagi, Y., Muramatsu, I.
  • Journal Title: Life Sci 78 Pages: 2454-2462
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Recent advances in technologies to analyze protein kinases. (2006)
  • Author(s): Ishida, A, Kameshita, I., Sueyoshi, N., Taniguchi, T., Shigeri Y.
  • Journal Title: J Pharmacol Sci (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Characteristics of acid extrusion from Chinese hamster ovary cells expressed different prostaglandin EP receptors. (2006)
  • Author(s): Okada, Y., Taniguchi T, Morishima, S., Suzuki, F., Akagi, Y.Muramatsu, I.
  • Journal Title: Life Sci 78 Pages: 2454-2462
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Recent advances in technologies to analyze protein kinases. (2006)
  • Author(s): Ishida, A, Kameshita, I., Sueyoshi, N., Taniguchi, T., Shigeri Y.
  • Journal Title: J Pharmacol Sci. (inpress)
• [Journal Article] Generation and application of a monoclonal antibody that detects a wide variety of protein tyrosine kinases. (2005)
  • Author(s): Sugiyama Y, Sueyoshi N, Shigeri Y, Tatsu Y, Yumoto N, Ishida A, Taniguchi T, Kameshita I
  • Journal Title: Anal Biochem. 347 Pages: 112-120
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Thromboxane A2 and prostaglandin F2alpha mediate inflammatory tachycardia. (2005)
  • Author(s): Takayama, K., Yuhki, K., Ono, K., Fujino, T., Hara, A., Yamada, T., Kuriyama, S., Karibe, H., Okada, Y., Takahata, O., Taniguchi T., Iijima, T., Iwasaki, H., Narumiya, S., Ushikubi, F.
  • Journal Title: Nat Med 11 Pages: 562-566
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification of major Ca(2+)/calmodulin-dependent protein kinase phosphatase-binding proteins in brain : biochemical analysis of the interaction. (2005)
  • Author(s): Ishida, A., Tada, Y., Nimura, T., Sueyoshi, N., Katoh, T., Takeuchi, M., Fujisawa, H., Taniguchi T, Kameshita, I.
  • Journal Title: Arch Biochem Biophys 435 Pages: 134-146
  • NAID: 120003132260
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Generation and application of a monoclonal antibody that detects a wide variety of protein tyrosine kinases. (2005)
  • Author(s): Sugiyama Y, Sueyoshi N, Shigeri Y, Tatsu Y, Yumoto N, Ishida A, Taniguchi T, Kameshita I.
  • Journal Title: Anal Biochem 347 Pages: 112-20
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Thromboxane A2 and prostaglandin F2alpha mediate inflammatory tachycardia. (2005)
  • Author(s): Takayama, K., Yuhki, K., Ono, K., Fujino, T., Hara, A., Yamada, T., Kuriyama, S., Karibe, H., Okada, Y., Takahata, O., Taniguchi T, Iijima, T., Iwasaki, H., Narumiya, S., Ushikubi, F.
  • Journal Title: Nat Med 11 Pages: 562-6
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Generation and application of a monoclonal antibody that detects a wide variety of protein tyrosine kinases. (2005)
  • Author(s): Sugiyama Y, Sueyoshi N, Shigeri Y, Tatsu Y, Yumoto N, Ishida A, Taniguchi T, Kameshita I.
  • Journal Title: Anal Biochem. 347 Pages: 112-120
• [Journal Article] Characteristics of acid extrusion from Chinese hamster ovary cells expressed different prostaglandin EP receptors. (2005)
  • Author(s): Okada, Y., Taniguchi T, Morishima, S., Suzuki, F., Akagi, Y., Muramatsu, I.
  • Journal Title: Life Sci (in press)
• [Patent(Industrial Property Rights)] 抗チロシンキナーゼ抗体およびその利用 (2005)
  • Inventor(s): 石田敦彦, 谷口隆信他
  • Industrial Property Rights Holder: 香川大学産総研, 旭川医大
  • Industrial Property Number: 2005-190494
  • Filing Date: 2005-06-29
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] 抗チロシンキナーゼ抗体およびその利用 (2005)
  • Inventor(s): 石田 敦彦, 谷口 隆信, 他
  • Industrial Property Rights Holder: 香川大学, 産総研, 旭川医大
  • Industrial Property Number: 2005-190494
  • Filing Date: 2005-06-29