| Project/Area Number |
17590322
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Fukuoka University |
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Principal Investigator |
TAKESHITA Morishige Fukuoka University, School of Medicine, Professor, 医学部, 教授 (90171636)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Nobuhiro Fukuoka University, University hospital, Instructor, 病院・講師 (40136445)
WATANABE Hiroshi Fukuoka University, School of Medicine, Instructor, 医学部, 講師 (40220917)
NIMUAR Satoshi Fukuoka University, School of Medicine, Assistant, 医学部, 助手 (10277064)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2006: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Pathology / γ / δ T cell lymphoma / Southern blot analysis |
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| Research Abstract |
γ/δT cells are mainly located in the liver and intestine. Over than half of the T-acute lymphoblastic leukemia cases show rearrangement of V δ1Jδ1and V δ 2J δ 1. It's reported that peripheral γ/δ T cell neoplasm rarely occurred and the primary tumors were mainly located in the liver and skin. The hepatic and cutaneousγ/δ T cell lymphomas were derived from V δ1Jδ 1 and V δ 2J δ 1 T cells, respectively. We tried to detect the distribution and the cell characteristics of γ/δ T cell lymphoma by the immunohistology and Southern blot analysis (SBA) of TCRJ δ and Cβ genes. 78 cases of peripheral T cell lymphoma except for ATL/L and cutaneous T cell lymphoma were examined. The primary sites of the lymphoma were as follows : liver and spleen (6 cases), intestine (7), nasal cavity (15), skin (20) and lymph node (30). By the SBA, only one cutaneous case showed rearrangement of V δ 1J δ 1/V δ 2D δ 3 in the 78 cases. The tumor cells were composed of TCR β F1-negative, γ/δ-positive, CD30-positive, CD4-positive anaplastic large cell lymphoma (ALCL). Detailed examination was performed in the additional 21 cases of systemic ALCL and 8 cutaneous ALCL, among them systemic 8 cases and cutaneous 3 showed γ/δ T cell phenotype. By the SBA, rearrangement of TCR β gene was detected in each 4 cases of cutaneous and systemic ALCL, and no rearrangement of TCR δ gene was seen. From the findings, it was suggested that rearrangement of TCR δ gene was rarely present in the cases of phenotypic γ/δ T cell lymphoma as well as examined hepatosplenic and cutaneous T-cell cases. NK cell and Null cell lymphomas may include in the cases of phenotypic TCR β F1-negative or γ/δ T cell lymphomas.
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