The analysis of PDGFR-β deficient brain

• Project/Area Number: 17590338
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: University of Toyama
• Principal Investigator: ISHII Yoko University of Toyama, University of Thyama, Graduate School of Medicine and Pharmaceutical Sciences for Education, Assistant Professor (00361949)
• Co-Investigator(Kenkyū-buntansha): SASAHARA Masakiyo University of Toyama, Graduate School of Medicine and Pharmaceutical Sciences for Education, Professor (20154015) ; ISHIZAWA Shin University of Toyama, Graduate School of Medicine and Pharmaceutical Sciences for Education, Associate Professor (40222989)
• Project Period (FY): 2005 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥3,860,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000); Fiscal Year 2005: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: platelet-derived growth factor / receptor / conditional knockout / neurotrophic factor / injury / excitotoxicity / differentiation / cell culture

Research Abstract

Platelet-derived growth factors (PDGFs) and PDGF receptors (PDGFRs) are widely expressed in the mammalian CNS, though their functional significance remains unclear. To clarify PDGFR function in CNS, we developed novel mutant mice in which the gene encoding the b subunit of PDGFR (PDGFR-b) was genetically deleted in CNS neurons to elucidate the role of PDGFR-b, particularly in the post-natal stage and analyzed the mutant mice.
1) The cerebral damage after cryogenic injury was severely exacerbated in the mutants compared with controls. Furthermore, TdT-mediated dUTP-biotin nick end labeling (TUNEL)- positive neuronal cell death and lesion formation in the cerebral hemisphere were extensively exacerbated in our mutant mice after direct injection of NMDA without altered NMDA receptor expression. Our results clearly demonstrate that PDGFR-b expressed in neurons protects them from cryogenic injury and NMDA-induced excitotoxicity.
2) We examined the inhibitory effect of PDGF-BB on the AMPA rece ptor function in the nucleus tractus solitarius (NTS) by using slice patch-clamp techniques. PDGF-BB significantly reduced the amplitude of AMPA-induced currents in NTS neurons, which showed that PDGF-BB could suppress the AMPA receptor-mediated excitatory input via the postsynaptic mechanism. The inhibitory effect of PDGF-BB on EPSCs was not observed in mutant mice with conditional deletion of the PDGFR-β gene in neurons. These studies suggest that the PDGF-B/PDGFR-β axis inhibits the AMPA receptormediated synaptic transmission that comprises the major part of the primary afferent to the NTS second-order neuron. The detected inhibitory action may be involved in the CNS regulation of the respiratory response.
3) PDGFRB-/-neural stem cells showed a higher rate of apoptosis without any significant decrease in proliferation. They demonstrated reduced capacities of migration and neuronal differentiation in response to not only PDGF-BB but also bFGF. bFGF increased the activity of the PDGFRB promoter as well as the expression and phosphorylation of PDGFRB. These results suggest the presence of the signaling convergence between PDGF and FGF. PDGFRB is needed for survival, and the effects of bFGF in migration and neural differentiation of the cells may be potentiated by induction of PDGFRB.

Research Products

• [Journal Article] Characterization of neuroprogenitor cells expressing the PDGF β-receptor within the subventricular zone of postnatal mice. (2008)
  • Author(s): Ishii Y., Matsumoto Y., Watanabe R., Elmi M., Fujimori T., Nissen J., Cao Y., Nabeshima Y., Sasahara M., Funa K.
  • Journal Title: Molecular and Cellular Neuroscience 37 Pages: 507-518
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] PDGF Receptor β is a Potent Regulator of Mesenchymal Stromal Cell Function. 2008 . (2008)
  • Author(s): Tokunaga A., Oya T., Ishii Y., Moto mura H, Nakamura C., Ishizawa S., Fujimori T., Nabeshima Y., Umezawa A., Kanamori M., Kimura T., Sasahara M.
  • Journal Title: Journal of Bone and Mineral Research (In press)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Characterization of neuroprogenitor cells expressing the PDGF 13-receptor within the subventricular zone of postnatal mice (2008)
  • Author(s): Ishii, Y., Matsumoto, Y., Watanabe, R., Elmi, M., Fujimori, T., Nissen, J., Cao, Y., Nabeshima, Y., Sasahara, M., Funa, K
  • Journal Title: Molecular and Cellular Neuroscience 37 Pages: 507-518
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] PDGF Receptor 13 is a Potent Regulator of Mesenchymal Stromal Cell Function (2008)
  • Author(s): Tokunaga, A. Oya, T., Ishii, Y., Motomura, H, Nakamura, C., Ishizawa, S., Fujimori, T., Nabeshima, Y., Umezawa, A. Kanamori, M., Kimura, T., Sasahara, M
  • Journal Title: Journal of Bone and Mineral Research (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Characterization of neuroprogenitor cells expressing the PDGF β-receptor within the subventricular zone of postnatal mice (2008)
  • Author(s): Ishii Y., Matsumoto Y., Watanabe R., Elmi M., Fujimori T., Nissen J., Cao Y., Nabeshma Y., Sasahara M., Funa K.
  • Journal Title: Molecular and Cellular Neuroscience 37 Pages: 507-518
  • Peer Reviewed
• [Journal Article] Neuroprotective Role of Transgenic PAF-Acetylhydrolase II in Mouse Models of Focal Cerebral Ischemia. (2007)
  • Author(s): Umemura K., Kato I., Hirashima Y., Ishii Y., Inoue T., Aoki J., Kono N., Oya T. Hayashi N., Hamada H., Endo S., Oda M., Arai H., Kinouchi H., and Hiraga K.
  • Journal Title: Stroke. 38 Pages: 1063-1068
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Platelet-derived growth factor(PDGF)-BB inhibits AMPA receptor-mediated synaptic transmission via PDGF receptor-beta in murine nucleus tractus solitarius. (2007)
  • Author(s): Ohi Y., Ishii Y., Haji A., Noguchi S., Sasaoka T., Fujimori T., Nabeshima Y, Sasahara M. Hattori Y.
  • Journal Title: Brain research 1159 Pages: 77-85
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Neuroprotective Role of Transgenic PAF-Acetylhydrolase II in Mouse Models of Focal Cerebral Ischemia. (2007)
  • Author(s): Umemura, K., Kato, I., Hirashima, Y., Ishii, Y., Inoue, T., Aoki, J., Kono, N., Oya, T., Hayashi, N., Hamada, H., Endo, S., Oda, M., Arai, H., Kinouchi, H., Hiraga, K
  • Journal Title: Stroke 38 Pages: 1063-1068
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Platelet-derived growth factor(PDGF)-BB inhibits AMPA receptor-mediated synaptic transmission via PDGF receptor-beta in murine nucleus tractus solitarius (2007)
  • Author(s): Ohi, Y., Ishii, Y., Haji, A. Noguchi, S., Sasaoka, T., Fujimori, T., Nabeshima, Y, Sasahara, M., Hattori, Y
  • Journal Title: Brain research 1159 Pages: 77-85
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Platelet-derived growth factor(PDGF)-BB inhibits AMPA receptor-mediated synaptic transmission via PDGF receptor-beta in murine nucleus tractus solitarius (2007)
  • Author(s): Ohi Y., Ishii Y., Haji A., Noguchi S., Sasaoka T, Fujimori T., Nabeshima Y, Sasahara M. HattoriY.
  • Journal Title: Brain research 1159 Pages: 77-85
  • Peer Reviewed
• [Journal Article] Neuroprotective role of transgenic PAF-acetylhydrolase II in mouse models of focal cerebral ischemia (2007)
  • Author(s): Umemura K., Kato I., Hirashima Y., Ishii Y., Inoue T., Aoki J, Kono N, Oya T., Hayashi N., Hamada H., Endo S
  • Journal Title: Stroke 38 Pages: 1063-1068
  • Peer Reviewed
• [Journal Article] Neuroprotective Role of Transgenic PAF-Acetylhydrolase II in Mouse Models of Focal Cerebral Ischemia. (2007)
  • Author(s): Umemura K., Kato I., Hirashima Y., Ishii Y., Inoue T., Aoki J., Kono N., Oya T., Hayashi N., Hamada H., Endo S., Oda M., Arai H., Kinouchi H., Hiraga K.
  • Journal Title: Stroke. 38 Pages: 1063-1068
• [Journal Article] Mouse brains deficient in neuronal PDGF receptor-beta develop normally but are vulnerable to injury. (2006)
  • Author(s): Ishii Y., Oya T., Zheng L., Gao Z., Kawaguchi M., Sabit H., Matsushima T., Tokunaga A., Ishizawa S., Hori E., Nabeshima Y., Sasaoka T., Fujimori T., Mori H. and Sasahara M.
  • Journal Title: J. Neurochem. 98 Pages: 588-600
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] A Homeodomain-Zinc Finger Protein, ZFHX4, Is Expressed in Neuronal Differentiation Manner and Suppressed in Muscle Differentiation Manner. (2006)
  • Author(s): Hemmi K., Ma D., Miura Y., Kawaguchi M., Sasahara M., Hashimoto-Tamaoki T., Tamaoki T., Sakata N. and Tsuchiya K
  • Journal Title: Biol. Pharm. Bull. 29 Pages: 1830-1835
  • NAID: 110005602346
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Absence of human herpesvirus-8 and Epstein-Barr virus in inflammatory myofibroblastic tumor with anaplastic large cell lymphoma kinase fusion gene. (2006)
  • Author(s): Yamamoto H., Kohashi K., Oda Y., Tamiya S., Takahashi Y., Kinoshita Y., Ishizawa S., Kubota M. and Tsuneyoshi M
  • Journal Title: Pathol. Int. 56 Pages: 584-590
  • NAID: 10019268620
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Mouse brains deficient in neuronal PDGF receptor-beta develop normally but are vulnerable to injury (2006)
  • Author(s): Ishii, Y., Oya, T., Zheng, L., Gao, Z., Kawaguchi, M., Sabit, H., Matsushima, T., Tokunaga, A. Ishizawa, S., Hori, E., Nabeshima, Y., Sasaoka, T., Fujimori, T., Mori, H., Sasahara, M
  • Journal Title: J. Neurochem 96 Pages: 588-600
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A Homeodomain-Zinc Finger Protein, ZFHX4, Is Expressed in Neuronal Differentiation Manner and Suppressed in Muscle Differentiation Manner (2006)
  • Author(s): Hemmi, K., Ma, D., Miura, Y., Kawaguchi, M., Sasahara, M., Hashimoto-Tamaoki, T., Tamaoki, T., Sakata, N., Tsuchiya K
  • Journal Title: Biol. Pharm. Bull 56 Pages: 1830-1835
  • NAID: 110005602346
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Absence of human herpesvirus-8 and Epstein-Barr virus in inflammatory myofibroblastic tumor with anaplastic large cell lymphoma kinase fusion gene (2006)
  • Author(s): Yamamoto, H., Kohashi, K., Oda, Y., Tamiya, S., Takahashi, Y., Kinoshita, Y., Ishizawa, S., Kubota, M., Tsuneyoshi, M
  • Journal Title: Pathol. Int 56 Pages: 584-590
  • NAID: 10019268620
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Mouse brains deficient in neuronal PDGF receptor-beta develop normally but are vulnerable to injury. (2006)
  • Author(s): Ishii Y., Oya T., Zheng L., Gao Z., Kawaguchi M., Sabit H., Matsushima T., Tokunaga A., Ishizawa S., Hori E., Nabeshima Y., Sakaoka T., Fujimori T., Mori H., Sasahara M.
  • Journal Title: J. Neurochem. 98 Pages: 588-600
• [Journal Article] A Homeodomain-Zinc Finger Protein, ZFHX4, Is Expressed in Neuronal Differentiation Manner and Suppressed in Muscle Differentiation Manner. (2006)
  • Author(s): Hemmi K., Ma D., Miura Y., Kawaguchi M., Sasahara M., Hashimoto-Tamaoki T., Tamaoki T., Sakata N., Tsuchiya K.
  • Journal Title: Biol. Pharm. Bull. 29 Pages: 1830-1835
  • NAID: 110005602346
• [Journal Article] Suppression of Epstein-Barr nuclear antigen 1 (EBNA1) by RNA interference inhibits proliferation of EBV-positive Burkitt's lymphoma cells. (2006)
  • Author(s): Hong M., Murai Y., Kutsuna T., Takahashi H., Nomoto K., Cheng C.M., Ishizawa S., Zhao Q.L., Ogawa R., Harmon B.V., Tsuneyama K., Takano Y.
  • Journal Title: J. Cancer Res. Clin. Oncol. 132 Pages: 1-8
• [Journal Article] Absence of human herpesvirus-8 and Epstein-Barr Virus in inflammatory myofibroblastic tumor with anaphastic large cell lymphoma kinase fusion gene. (2006)
  • Author(s): Yamamoto H., Kohashi K., Oda Y., Tamiya S., Takahashi Y., Kinoshita Y., Ishizawa S., Kubota M., Tsuneyoshi M
  • Journal Title: Pathol. Int. 56 Pages: 584-590
• [Journal Article] Deletion of The PDGFR-β gene affects key fibroblast functions important for wound healing. (2005)
  • Author(s): Gao Z., Sasaoka T., Fujimori T., Oya T., Ishii Y., Sabit H., Kawaguchi M., Kurotaki Y., Naito M., Wada T., Ishizawa S., Kobayashi M., Nabeshima Y. I. and Sasahara M
  • Journal Title: J. Biol. Chem. 280 Pages: 9375-9389
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Some biochemical and histochemical properties of human liver serine dehydratase. (2005)
  • Author(s): Kashii T., Gomi T., Oya T., Ishii Y., Oda H., Maruyama M., Kobayashi M., Masuda T., Yamazaki M., Nagata T., Tsukada K., Nakajma A., tatsu K., Mori H., Takusagawa F., Owa H. and Pitot H. C.
  • Journal Title: Int. J. Biochem. Cell Biol. 37 Pages: 574-589
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Cytoplasmic expression of c-erbB2 in non-small cell lung cancers. (2005)
  • Author(s): Cheng C. M., Tsuneyama K., Matsui K., Takahashi H., Ishizawa S. and Takano Y
  • Journal Title: Virchows Arch 446 Pages: 596-603
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Deletion of The PDGFR-B gene affects key fibroblast functions important for wound healing (2005)
  • Author(s): Gao, Z., Sasaoka, T., Fujimori, T., Oya, T., Ishii, Y., Sabit, H., Kawaguchi, M., Kurotaki, Y., Naito, M., Wada, T., Ishizawa, S., Kobayashi, M., Nabeshima, Y.I., Sasahara, M
  • Journal Title: J. Biol. Chem 280 Pages: 9375-9389
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Some biochemical, and histochemical properties of human liver serine dehydratase (2005)
  • Author(s): Kashii, T., Gomi, T., Oya, T., Ishii, Y., Oda, H., Maruyama, M., Kobayashi, M., Masuda, T., Yamazaki, M., Nagata, T., Tsukada, K., Nakajima, A. Tatsu, K., Mori, H., Takusagawa, F., Ogawa, H., Pitot, H.C
  • Journal Title: Int. J. Biochem. Cell Biol 37 Pages: 574-589
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cytoplasmic expression of c-erbB2 in non-small cell lung cancers (2005)
  • Author(s): Cheng, C.M., Tsuneyama, K., Matsui, K., Takahashi, H., Ishizawa, S., Takano, Y
  • Journal Title: Virchows Arch 446 Pages: 596-603
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Deletion of The PDGFR-β gene affects key fibroblast functions important for wound healing. (2005)
  • Author(s): Gao Z., Sasaoka T., Fujimori T., Oya T., Ishii Y., Sabit H., Kawaguchi M., Kurotaki Y., Naito M., Wada T., Ishizawa S., Kobayashi M., Nabeshima Y., Sasahara M.
  • Journal Title: J.Biol.Chem. 280(10) Pages: 9375-9389
• [Journal Article] Some biochemical and histochemical properties of human liver serine dehydratase. (2005)
  • Author(s): Kashii T., Gomi T., Oya T., Ishii Y., Oda H., Maruyama M., Kobayashi M., Masuda T., Yamazaki M., Nagata T., Tsukada K., Nakajima A., Tatsu K., Mori H., Takusagawa F., Ogawa H., Pitot H.C
  • Journal Title: Int.J.Biochem.Cell Biol. 37(3) Pages: 574-589
• [Journal Article] Cytoplasmic expression of c-erbB2 in non-small cell lung cancers. (2005)
  • Author(s): Cheng C.M., Tsuneyama K., Matsui K., Takahashi H., Ishizawa S., Takano Y.
  • Journal Title: Virchows Archv. 446(6) Pages: 596-603
• [Journal Article] Suppression of Epstein-Barr nuclear antigen 1(EBNA1) by RNA interference inhibits proliferation of EBV-positive Burkitt's lymphoma cells. (2005)
  • Author(s): Hong M., Murai Y., Kutsuna T., Takahashi H., Nomoto K., Cheng C.M., Ishizawa S., Zhao Q.L., Ogawa R., Harmon B.V., Tsuneyama K., Takano Y.
  • Journal Title: J Cancer Res Clin Oncol 132(1) Pages: 1-8
• [Journal Article] FH4 protein is expressed in many neurons of developing rat brain. (2005)
  • Author(s): Nogami S., Ishii Y., Kawaguchi M., Sakata N., Oya T., Takagawa K., Kanamori M., Sabit H., Obata T, Kimura T., Sasahara M.
  • Journal Title: J Comp Neurol 482(1) Pages: 33-49
• [Journal Article] 神経組織に発現する血小板由来増殖因子. (2005)
  • Author(s): 笹原正清, 尾矢剛志, 石井陽子, 石澤伸
  • Journal Title: 生体の科学 56(4) Pages: 292-295
• [Presentation] Transgenic PAF-Acetylhydrolase II Protects Neurons in Mouse Models of Transient Focal Cerebral Ischemia. (2007)
  • Author(s): Kato I., Umemura K., Ishii Y., Inoue T., Aoki J., Kono N., Oya T., Arai H., Hayashi N., Hamada H., Endo S., Hirashima Y. and Hiraga K.
  • Organizer: 21COE Program Joint symposium of Peking University and University of Toyama. "Evidence-based approach to traditional medicine and modern medicine"
  • Place of Presentation: Beijing
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] PDGF-Bは酸化ストレス誘導性細胞死から神経細胞を保護し、その作用はPI3K/AKT経路を介する (2007)
  • Author(s): 鄭蓮順, 石井陽子, 尾矢剛志, アムラサビット, 石澤伸, 松島貴子, 倉茂洋一, 笹原正清
  • Organizer: 第96回日本病理学会総会
  • Place of Presentation: 大阪
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] マウス星細胞活性化における血小板由来増殖因子β受容体の役割 (2007)
  • Author(s): 森田誠市, 尾矢剛志, 石井陽子, アムラサビット, 徳永綾乃, 石澤 伸, 松島貴子, 倉茂洋一, 笹原正清
  • Organizer: 第96回日本病理学会総会
  • Place of Presentation: 大阪
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 皮膚培養線維芽細胞の遊走におけるPDGFβ受容体の機能解析 (2007)
  • Author(s): 徳永綾乃, 尾矢剛志, 石井陽子, アムラサビット, 松島貴子, 倉茂洋一, 石澤 伸, 金森昌彦, 笹原正清
  • Organizer: 第96回日本病理学会総会
  • Place of Presentation: 大阪
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Neuroprotection of PDGF-B against oxidative stress via the PI3K/Akt signal. (2007)
  • Author(s): 鄭蓮順, 石井陽子, 尾矢剛志, 徳永綾乃, 濱島丈, 石澤伸, 笹原正清
  • Organizer: Neuro 2007(第30回日本神経科学会第50回日本神経化学会合同大会)
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Transgenic PAF-Acetylhydrolase II Protects Neurons in Mouse Models of Transient Focal Cerebral Ischemia. 21COE Program Joint symposium of Peking University and University of Toyama (2007)
  • Author(s): Kato, I., Umemura, K., Ishii, Y., Inoue, T., Aoki, J., Kono, N., Oya, T., Arai, H., Hayashi, N., Hamada, H., Endo, S., Hirashima, Y., Hiraga, K
  • Organizer: Evidence-based approach to traditional medicine and modern medicine
  • Place of Presentation: Beijing
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] PDGF-B protects neuron from oxidative stress via the PI3K/Akt signal (2007)
  • Author(s): Zheng, L., Ishii, Y., Oya, T., Sabit, H., Matsushima, T., Kurashige, Y., Ishizawa, S., Sasahara, M
  • Organizer: The 96th Annual Meeting of the Japan Society of Pathology
  • Place of Presentation: Osaka
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] The role of PDGFR-t3 in the activation of mouse stellate cells (2007)
  • Author(s): Morita, S., Oya, T., Ishii, Y., Sabit, H., Tokunaga, A. Ishizawa, S., Matsushima, T., Kurashige, Y., Sasahara, M
  • Organizer: The 96th Annual Meeting of the Japan Society of Pathology
  • Place of Presentation: Osaka
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] The analysis of the function of PDGFR-13 in fibroblast migration (2007)
  • Author(s): Tokunaga, A. Oya, T., Ishii, Y., Sabit, H., Matsushima, T., Kurashige, Y., Ishizawa, S., Kanamori, M., Sasahara, M
  • Organizer: The 96th Annual Meeting of the Japan Society of Pathology
  • Place of Presentation: Osaka
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Neuroprotection of PDGF-B against oxidative stress via the PI3K/Akt signal (2007)
  • Author(s): Zheng, L., shii, Y., Oya, T., Tokunaga, A. Hamashima, T., Ishizawa, S., Sasahara, M
  • Organizer: Neuro 2007(The 29th Annual Meeting of the Japan Neuroscience Society, The 50th Annual Meeting of the Japanese Society for Neurochemistry
  • Place of Presentation: Yokohama
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Neuroprotection of PDGF-B agatnst oxidative stress via the PI3K/Akt signal (2007)
  • Author(s): 鄭 蓮順, 石井 陽子, 尾矢 剛志, 徳永 綾乃, 濱島 丈, 石澤 伸, 笹原 正清
  • Organizer: Neuro 2007(第30回日本神経科学会第50回日本神経化学会合同大会)
  • Place of Presentation: 横浜
• [Presentation] Overexpression of PAF-acetylhydrolase II in transgenic mouse brain supresses ischemic stress-induced neuronal cell death. (2006)
  • Author(s): Umemura K., Kato I., Hirashima Y., Ishii Y., Oya T., Hayashi N., Hamada H., Inoue T., Aoki J., Kinouchi H., Hiraga K. and Endo S
  • Organizer: 2006 AANS Annual Meeting
  • Place of Presentation: San Francisco CA, America
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 末梢神経再生におけるPDGF受容体およびシグナル伝達に関する検討. (2006)
  • Author(s): 山崎隆志, 尾矢剛志, Sabit H., 石井陽子, 石澤伸, 笹原正清
  • Organizer: 第95回日本病理学会
  • Place of Presentation: 東京
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] A novel model for diabetic retinopathy. (2006)
  • Author(s): Ichsan A. M., Kato I., Oya T., Tomi M., Takasawa S., Okamoto H., Sasahara M., Hayasaka S., Hosoya K. and Hiraga K.
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
  • Place of Presentation: Kyoto
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Expression of constitutively active form of CaM kinase II in pancreatic beta cells of transgenic mice leads to severe diabetes mellitus. (2006)
  • Author(s): Kato I., Hiraga K., Oya T., Sasahara M., Takasaki I., Tabuchi Y., Takasawa S. and Okamoto H
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
  • Place of Presentation: Kyoto
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 末梢神経再生におけるPDGF受容体およびそのシグナル伝達に関する検討. (2006)
  • Author(s): Sabit H., Yamazaki T., Oya T., Ishii Y., Furuta I. and Sasahara M.
  • Organizer: 第29回日本神経科学大会(Neuro2006)
  • Place of Presentation: 京都
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Mouse brains deficient in neuronal PDGF receptor-β develop normally but are vulnerable to injury. (2006)
  • Author(s): Ishii Y., Oya T., Zheng L., Sasahara M.
  • Organizer: 第29回日本神経科学大会(Neuro2006)
  • Place of Presentation: 京都
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] The role of platelet-derived growth factor receptor β in the process of mouse hepatic stellate cell activation. (2006)
  • Author(s): Morita S., Sabit H., Oya T., Tokunaga A., Kudo H., Yata Y., Takahara T., Ishii Y., Ishizawa S., Tsukada K. and Sasahara M.
  • Organizer: 13th International Symposium on Cells of the Hepatic Sinusoid
  • Place of Presentation: Niigata
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] AMPA受容体を介したモルモット延髄孤束核における興奮性シナプス後電流に対するPDGF-Bの作用:ノックアウトマウスを用いた検討. (2006)
  • Author(s): 大井義明, 石井陽子, 櫨彰, 笹原正清, 服部裕一
  • Organizer: 第57回日本薬理学会北部会
  • Place of Presentation: 弘前
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Overexpression of PAF-acetylhydrolase II in transgenic mouse brain supresses ischemic stress-induced neuronal cell death (2006)
  • Author(s): Umemura, K., Kato, I., Hirashima, Y., Ishii, Y., Oya, T., Hayashi, N., Hamada, H., Inoue, T., Aoki, J., Kinouchi, H., Hiraga, K., Endo, S
  • Organizer: 2006 AANS Annual Meeting
  • Place of Presentation: 4 San Francisco CA, America
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] The analysis of PDGF b receptor and its signaling in peripheral nervous regeneration (2006)
  • Author(s): Yamazaki, T., Oya, T., Sabit, H. Ishii, Y., Ishizawa, S., Sasahara, M
  • Organizer: The 95th Annual Meeting of the Japan Society of Pathology
  • Place of Presentation: Tokyo
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] A novel model for diabetic retinopathy (2006)
  • Author(s): Ichsan, A.M., Kato, I., Oya, T., Tomi, M., Takasawa, S., Okamoto, H., Sasahara, M., Hayasaka, S., Hosoya, K., Hiraga, K
  • Organizer: The 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
  • Place of Presentation: Kyoto
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Expression of constitutively active form of CaM kinase II in pancreatic beta cells of transgenic mice leads to severe diabetes mellitus (2006)
  • Author(s): Kato, I., Hiraga, K., Oya, T., Sasahara, M., Takasaki, I., Tabuchi, Y., Takasawa, S., Okamoto, H
  • Organizer: The 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
  • Place of Presentation: Kyoto
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Study about PDGF receptors and their signaling in peripheral nervous regeneration (2006)
  • Author(s): Sabit, H., Yamazaki, T., Oya, T., Ishii, Y., Furuta, I., Sasahara, M
  • Organizer: The 29th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Kyoto
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Mouse brains deficient in neuronal PDGF receptor-beta develop normally but are vulnerable to injury (2006)
  • Author(s): Ishii, Y., Oya, T., Zheng, L., Sasahara, M
  • Organizer: The 29th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Kyoto
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] The role of platelet-derived growth factor receptor 13 in the process of mouse hepatic stellate cell activation (2006)
  • Author(s): Morita, S., Sabit, H., Oya, T., Tokunaga, A. Kudo, H., Yata, Y., Takahara, T., Ishii, Y., Ishizawa, S., Tsukada, K., Sasahara, M
  • Organizer: 13th International Symposium on Cells of the Hepatic Sinusoid
  • Place of Presentation: Niigata
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Platelet-derived growth factor(PDGF)-BB inhibits AMPA receptor-mediated synaptic transmission via PDGF receptor-beta in murine nucleus tractus solitarius (2006)
  • Author(s): Ohi, Y., Ishii, Y., Haji, A. Sasahara, M. Hattori, Y
  • Organizer: The 57th Annual Meeting of the Japanese Pharmacological Society, Northern Branch
  • Place of Presentation: Hirosaki
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] A study on proliferation, migration and apoptosis after PDGF-β knockout in dermal fibroblasts. (2005)
  • Author(s): 笹原正清, Zhi-Yang Gao, 笹岡利安, 尾矢剛志, 石井陽子, Hemragul Sabit, 石澤伸
  • Organizer: 第94回日本病理学会総会
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] The activation of ERK1/2 and JNK, but not p38, was attenuated after PDGFR-β depletion. (2005)
  • Author(s): Zhi-Yang Gao, 笹原正清, 笹岡利安, 尾矢剛志, 石井陽子, Hemragul Sabit, 石澤伸
  • Organizer: 第94回日本病理学会総会
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Cre-loxPノックアウトシステムを用いた皮膚線維芽細胞におけるPDGFR-β機能の解析. (2005)
  • Author(s): 尾矢剛志, Zhi-Yang Gao, 石井陽子, 笹岡利安, Hemragul Sabit, 徳永綾乃, Lian-Shun Zheng, 石澤伸, 藤森俊彦, 笹原正清
  • Organizer: 第37回日本結合組織学会学術大会
  • Place of Presentation: 富山
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Establishment of mutant mouse with neuron-specific PDGF receptor depletion. (2005)
  • Author(s): Sasahara M., Gao Z., Oya T., Ishii Y., Sasaoka T., Ishizawa S., Matsushima T., Kurashige Y., Sabit H., Zheng L., Tokunaga A. and Fujimori T.
  • Organizer: 第28回日本神経科学大会
  • Place of Presentation: 横浜
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] A study on proliferation, migration and apoptosis after PDGF-13 knockout in dermal fibroblasts (2005)
  • Author(s): Sasahara, M., Gao, Z., Sasaoka, T., Oya, T., Ishii, Y., Sabit, H., Ishizawa, S
  • Organizer: The 94th Annual Meeting of the Japan Society of Pathology
  • Place of Presentation: Yokohama
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] The activation of ERK1/2 and JNK, but not p38, was attenuated after PDGFR-(3 depletion (2005)
  • Author(s): Gao, Z., Sasahara, M., Sasaoka, T., Oya, T., Ishii, Y., Sabit, H., Ishizawa, S
  • Organizer: The 94th Annual Meeting of the Japan Society of Pathology
  • Place of Presentation: Yokohama
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] The analysis of the PDGFR-I3 function in fibroblasts using Cre-loxP knockout system (2005)
  • Author(s): Oya, T., Gao, Z., Ishii, Y., Sasaoka, T., Sabit, H. Tokunaga, A. Zheng, L., Ishizawa, S., Fujimori, T., Sasahara, M
  • Organizer: The 37th Annual Meeting of the Japan Connective Tissue Society
  • Place of Presentation: Toyama
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Establishment of mutant mouse with neuron-specific PDGF receptor depletion (2005)
  • Author(s): Sasahara, M., Gao, Z., Oya, T., Ishii, Y., Sasaoka, T., Ishizawa, S., Matsushima, T., Kurashige, Y., Sabit, H., Zheng, L., Tokunaga, A. Fujimori, T
  • Organizer: The 28th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Yokohama
  • Description: 「研究成果報告書概要(欧文)」より
• [Remarks] 「研究成果報告書概要(和文)」より
  • URL: http://www.med.u-toyama.ac.jp/pathol2/index.html