Mechanisms of differentiation and maintenance of hematopoietic cells and their supporting microenvironment
| Project/Area Number |
17590346
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Tottori University |
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Principal Investigator |
HAYASHI Shin-ichi Tottori University, Faculty of Medicine, Professor, 医学部, 教授 (50208617)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | bone marrow / B lymphocyte / hematopoietic microenvironment / plasma cell / beta-catenin / stem cell / immunology / mouse / 血液幹細胞 / 破骨細胞 / 胚性幹(ES)細胞 / 髄外造血 / ストロマ細胞 / 大理石骨病 |
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| Research Abstract |
1)Mechanisms of the plasma cell migration from spleen to bone marrow.
To be clear the mechanisms of migration of plasma cells into bone marrow, the regulatory molecule was assessed. An antagonistic antibody against Flt3 (A2F10) injection at priming reduced the numbers of plasma cells in bone marrow, and increased in spleen. Antigen priming reduced the expression of CXCL12 on plasma cells in control mice, but not in A2F10-treated mice. Signaling via Flt3 on plasma cells reduced CXCL12, resulting in accelerating the migration of plasma cells to bone marrow. These results suggested that the regulation of CXCL12 expression by F1t3 signaling controlled plasma cell numbers in bone marrow. (Manuscript in preparation)
2)Function of Wnt/β-catenin signaling for multipotent hematopoietic cells.
To investigate one component of the Wnt/β-catenin signaling pathway that has been implicated in stem cell self-renewal. Retroviral-mediated introduction of stable β-catenin to primitive murine bone marrow cel
… More
ls allowed the expansion of multipotential c-Kit^<low>Sca-1^<low/->CD19^-CD11b^-Flt3^-CD43^+AA4.1^+NK1.1^-CD3^- CD11c^-Gr-1^-CD45R^+ cells in the presence of stromal cells and cytokines. These findings implicated the canonical Wnt pathway signaling in regulation of multipotent progenitors. (J. Immunol, 2006, 177: 2294-2303)
3)Characterization of multipotent cells in developing teeth.
To assess the potential of dental mesenchymal cells in developing teeth, we prepared mesenchymal cells from E13.5 tooth germ cells and assessed their potential for differentiation in culture. They differentiated into odontoblasts, chondrocyte-like cells, and osteoblast-like cells. Their derivation was confirmed by tracing NC-derived cells as LacZ^+ cells using P0-Cre/Rosa26R mice. These results suggest that NC-derived cells with the potential to differentiate into chondrocyte-like and osteoblast-like cells are present in the developing tooth, and these cells may contribute to tooth organogenesis. (Stem Cells, 2007, 25 : 78-87) Less
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Report
(3 results)
Research Products
(29 results)
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[Journal Article] Constitutively active P-catenin promotes expansion of multipotent hematopoietic progenitors in culture.2006
Author(s)
Baba, Y., Yokota, T., Spits, H., Garrett, K., Hayashi.S.I., Kincade, P.W.
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Journal Title
J. Immunol. 117
Pages: 2294-2303
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Enforced expression of PU.1 rescues osteoclastogenesis from embryonic stem cells lacking Tal-1.2005
Author(s)
Tsuneto, M., Tominaga, A., Yamazaki, H., Yoshino, M., Orkin, S.H., Hayashi.S.I.
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Journal Title
Stem Cells 23
Pages: 134-143
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Vascular endothelial growth factor receptor 1 signaling is essential for osteoclast development and bone-marrow formation in CSF-1-deficient mice.2005
Author(s)
Niida, S., Kondo, T., Hiratsuka, S., Hayashi, S.I..Amizuka, N., Noda, T., Ikeda, T., Shibuya, M.
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Journal Title
Proc. Natl. Acad. Sci. USA 102
Pages: 14016-14021
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Presence and distribution of neural crest-derived cells in the murine developing thymus and their potential for differentiation.2005
Author(s)
Yamazaki, H., Sakata, E., Yamane, T., Yanagisawa, A., Abe, K., Yamamura, K.I., Hayashi, S.I., Kunisada, T.
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Journal Title
Int. Immunol. 17
Pages: 549-558
NAID
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Down-regulation of osteoprotegerin production in bone marrow macrophages by macrophage colony-stimulating factor.2005
Author(s)
Yamada, N., Tsujimura, T., Ueda, H., Hayashi, S.I., Ohyama, H., Okamura, H., Terada, N.
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Journal Title
Cytokine 31
Pages: 288-297
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Fos plays no role in apoptosis of epithelia in the mouse male accessory sex organs and uterus.2005
Author(s)
Kuhara, A., Yamada, N., Sugihara, A., Ohyama, H., Tsujimura, T., Hayashi, S.I., Terada, N.
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Journal Title
Endocr. J. 52
Pages: 153-158
NAID
Description
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