| Project/Area Number |
17590353
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Oita University |
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Principal Investigator |
HIIYA Naoki Oita University, Faculty of Medicine, Assistant professor, 医学部, 助手 (80305036)
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| Co-Investigator(Kenkyū-buntansha) |
MORIYAMA Masatsugu Oita University, Faculty of Medicine, professor, 医学部, 教授 (90239707)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | ARPP / Knock-out mouse / skeletal muscle / muscle differentiation / muscle injury / muscle regeneration |
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| Research Abstract |
Recently, we identified a novel human protein, ankyrin-repeated protein with PEST and praline-rich region (ARPP). ARPP is characterized structurally by the presence of four ankyrin-repeated domains in its central portion. The amino acid sequence of ARPP is highly homologous with that of cardiac restricted ankyrin-repeated protein (CARP; 52%). We observed high expression levels of ARPP in skeletal muscle and the heart. ARPP was selectively expressed in type 1 myofibers of skeletal muscle and, therefore, ARPP-positive myofibers were arranged in a checkerboard-like pattern. In this study, we analyzed the function of ARPP in vivo by generating ARPP knock-out (KO) mouse. Our findings are as follows;
1) Compared with normal mouse, ARPP KO mouse showed decreased exercise capacity for endurance training
2) ARPP expression in the skeletal muscle was found to be induced by denervation for 10 weeks.
3) ARPP expression in the disuse atrophic skeletal muscle induced by tail-suspension was significantly down-regulated.
4) ARPP KO mouse showed distinct expression pattern of some genes expressed specifically in the nervous system as well as skeletal muscle specific genes.
5) ARPP KO mouse showed much more severe muscle injury induced by injection of cardiotoxin and delayed clearance of necrotic tissue compared with normal mouse
These findings suggest that ARPP might be concerned with the induced expression of the several proteins acting as the procetive effects in the muscle performance, muscle injury and muscle regeneration.
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