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Mechanistic analysis for androgen independent growth of prostate cancers

Research Project

Project/Area Number 17590356
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionNagoya City University

Principal Investigator

ASAMOTO Makoto  Nagoya City University, Graduate School of Medical Science, Associate Professor (50212494)

Co-Investigator(Kenkyū-buntansha) 外岩戸 尚美  名古屋市立大学, 大学院医学研究科, 研究員 (70381853)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,610,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsProstate cancer / cell culture / androgen / steroid hormone / cancer stem cells / 前立腺がん / 細胞株 / ラット / CD133 / GSTP / siRNA / 前立腺 / がん / トランスジェニック動物 / ヌードマウス / 可移植腫瘍 / マイクロアレイ
Research Abstract

We generated a transgenic rat with the SV40 T antigen under probasin promoter control, allowing prostate-specific gene expressions. Males demonstrate atypical epithelial cell proliferation in the prostate from 4 weeks of age and develop prostate carcinomas at 100% incidence before they are 15 weeks old. From the transgenic rat of 55 week-old, we established a transplanted tumor in nude mice with castration treatments. And then, we have tried to established cell lines from the nude mice tumor. Finally we established 3 cell lines. One cell line designated PCai-1 shows charcoal treated FBS insensitive growth, and two other cell lines PCai-2 and PCai-3 are sensitive for the charcoal treatment FBS with stopping growth. Characteristic gene expression profiles have been obtained for each 3 cell lines. These cell lines may be useful to study mechanisms of progression of prostate cancers, and to study chemo-prevention of prostate cancers. Furthermore, we established new cell lines from the non-castrated androgen dependent transplanted tumors. These cells had the stem cell like characters such as asymmetric tumor growth and CD133 expression.

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (49 results)

All 2008 2007 2006 2005

All Journal Article (45 results) (of which Peer Reviewed: 14 results) Presentation (2 results) Book (2 results)

  • [Journal Article] Glutathione S-transferase pi mediates proliferation of androgen independent prostate cancer cells2008

    • Author(s)
      Hokaiwado, N
    • Journal Title

      Carcinogenesis (Epub ahead of print)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Glutathione S-transferase pi mediates proliferation of androgen independent prostate cancer cells2008

    • Author(s)
      Hokaiwado, N., Takeshita, F., Naiki-Ito, A., Asamoto, M., Ochiya, T., Shirai, T
    • Journal Title

      Carcinogenesis., Epub ahead of print

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Glutathione S-transferase pi mediates proliferation of androgen independent prostate cancer cells.2008

    • Author(s)
      Hokaiwado, N.
    • Journal Title

      Carcinogenesis (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protective effects of citrus nobiletin and auraptene in transgenic rats developing adenocarcinoma of the prostate(TRAP) and human prostate carcinoma cells2007

    • Author(s)
      Tang, M
    • Journal Title

      Cancer Sci 98

      Pages: 471-477

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Protective effects of citrus nobiletin and auraptene in transgenic rats developing adenocarcinoma of the prostate (TRAP) and human prostate carcinoma cells2007

    • Author(s)
      Tang, M., Ogawa, K., Asamoto, M., Hokaiwado, N., Azman, S., Suzuki, S., Takahashi, S., Tanaka, T., Ichikawa, K., Shirai, T
    • Journal Title

      Cancer Sc 98

      Pages: 471-477

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Protective effects of citrus nobiletin and auraptene in transgenic rats developing adenocarcinoma of the prostate(TRAP) and human prostate carcinoma cells.2007

    • Author(s)
      Tang, M.
    • Journal Title

      Cancer Sci. 98

      Pages: 471-477

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Characterization and application of polycolonal antibodies that specifically recog-nize JC virus large T antigen2006

    • Author(s)
      Sumden, Y
    • Journal Title

      Acta.Neuropathol 111

      Pages: 379-387

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Inhibition of prostate carcinogenesis in probasin/SV40 T antigen transgenic rats by leuprorelin,a luteinizing hormone-releasing hormone agonist2006

    • Author(s)
      Ssid, M
    • Journal Title

      Cancer Sci 97

      Pages: 459-467

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Limitede effects of disrupted Gap Junctions in the liver on multi-organ carcinogenesis induced by Diisopropanolnitrosamine in the mutant Connexin 32 Transgenic rat2006

    • Author(s)
      Murasaki, T
    • Journal Title

      J Toxicol Pathol 19

      Pages: 93-97

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Closerelation of Transforming Growth Factor-α positive hepatic foci to hepatocel-lular tumor development in the rat2006

    • Author(s)
      Tamano, S
    • Journal Title

      J Toxicol Pathol 19

      Pages: 96-106

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Prediction of carcinogeni cpotential by a toxicogenomic approach using rat hepato-ma cells2006

    • Author(s)
      Tsujimura, K
    • Journal Title

      Cancer Sci 97

      Pages: 1002-1010

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Characterization and application of polycolonal antibodies that specifically recognize JC virus large T antigen2006

    • Author(s)
      Sunden, Y., Suzuki, T., Orba, Y., Umemura, T., Asamoto, M., Nagashima, K., Tanaka, S., Sawa, H
    • Journal Title

      Acta. Neuropathol 111

      Pages: 379-387

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Inhibition of prostate carcinogenesis in probasin/SV40 T antigen transgenic rats by leuprorelin, a luteinizing hormone-releasing hormone agonist2006

    • Author(s)
      Said, M., Hokaiwado, N., Tang, M., Ogawa, K., Suzuki, S., Ghanem, H. N., Esmat, A. Y., Asamoto, M., Fawzia, M. R., Shirai, T
    • Journal Title

      Cancer Sci 97

      Pages: 459-467

    • NAID

      10018176080

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Limited effects of disrupted Gap Junctions in the liver on multi-organ carcinogenesis induced by Diisopropanolnitrosamine in the mutant Connexin 32 Transgenic rat2006

    • Author(s)
      Murasaki, T., Asamoto, M., Hokaiwado, N., Ogawa, K., Shirai, T
    • Journal Title

      J Toxicol Pathol 19

      Pages: 93-97

    • NAID

      130000065694

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Close relation of Transforming Growth Factor a positive hepatic foci to hepatocellular tumor development in the rat2006

    • Author(s)
      Tamano, S., Kawabe, M., Ichihara, T., Hagiwara, A, Sano, M., Asamoto, M., Shirai, T
    • Journal Title

      J Toxicol Pathol 19

      Pages: 99-106

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Prediction of carcinogenic potential by a toxicogenomic approach using rat hepatoma cells2006

    • Author(s)
      Tsujimura, K., Asamoto, M., Shugo, S., Hokaiwado, N., Ogawa, K., Shirai, T
    • Journal Title

      Cancer Sci 97

      Pages: 1002-1010

    • NAID

      10019377814

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Characterization and application of polycolonal antibodies that specifically recognize JC virus large T antigen.2006

    • Author(s)
      Sunden, Y
    • Journal Title

      Acta. Neuropathol. 111

      Pages: 379-387

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Inhibition of prostate carcinogenesis in probasin/SV40 T antigen transgenic rats by leuprorelin, a luteinizing hormone-releasing hormone agonist.2006

    • Author(s)
      Said, M.
    • Journal Title

      Cancer Sci. 97

      Pages: 459-467

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Limited effects of disrupted Gap Junctions in the liver on multi-organ carcinogenesis induced by Diisopropanolnitrosamine in the mutant Connexin 32 Transgenic rat.2006

    • Author(s)
      Murasaki, T.
    • Journal Title

      J Toxicol Pathol. 19

      Pages: 93-97

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Close relation of Transforming Growth Factor-α positive hepatic foci to hepatocellular tumor development in the rat.2006

    • Author(s)
      Tamano, S.
    • Journal Title

      J Toxicol Pathol. 19

      Pages: 99-106

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Prediction of carcinogenic potential by a toxicogenomic approach using rat hepatoma cells.2006

    • Author(s)
      Tsujimura, K.
    • Journal Title

      Cancer Sci. 97

      Pages: 1002-1010

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Lack of modification of 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx) rat hepatocarcinogenesis by caffeine, a CYP1A2 inducer, points to complex counteracting influences.2006

    • Author(s)
      Kuribayashi, M.
    • Journal Title

      Cancer Lett., 232・2

      Pages: 289-299

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Differences Between Latent and Clinical Prostate Carcinomas : Lower Cell Proliferation Activity in Latent Cases.2006

    • Author(s)
      Takahashi, S.
    • Journal Title

      The Prostate 66

      Pages: 211-217

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Tbx3 expression is related to apoptosis and cell proliferation in rat bladder both hyperplastic epithelial cells and carcinoma cells2005

    • Author(s)
      Ito, A
    • Journal Title

      Cancer Lett 219

      Pages: 105-112

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Downregulation of apoptosis revealed by laser microdissection and cDNA microarray analysis of related genesin rat liver preneoplastic lesions2005

    • Author(s)
      Ogawa, K
    • Journal Title

      Med.Mol.Morphol 38

      Pages: 23-39

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Harman and norhannan suppressed but NaNO2 enhanced the development of preneoplastic livercell foci in 2-amino-3,8-dimethylimidazo[4,5-f] Jquinoxaline (MelQx) treated rats2005

    • Author(s)
      Sugiura, S
    • Journal Title

      J.Toxicol.Pathol 18

      Pages: 99-104

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Equivocal impact of transplacental and lactational exposure to a food-derived carcinogen,2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine,on prostate and colonlesion development in F344 rats2005

    • Author(s)
      Ikeda, Y
    • Journal Title

      Cancer Lett 224

      Pages: 23-30

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Influence of atrazineadministration and reduction of calorieintake on prostate carcinogenesis in probasin/SV40 T antigen transgenic rats2005

    • Author(s)
      Kandori, H
    • Journal Title

      Cancer Sci 96

      Pages: 221-226

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Lack of effects of 1439 MHz electromagnetic near field exposure on the blood-brain barrier in immature and young rats2005

    • Author(s)
      Kurinbayashi, M
    • Journal Title

      Bioelectromagnetics 26

      Pages: 578-588

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Tbx3 expression is related to apoptosis and cell proliferation in rat bladder both hyperplastic epithelial cells and carcinoma cells2005

    • Author(s)
      Ito, A., Asamoto, M., Hokaiwado, N., Takahashi, S., Shirai, T
    • Journal Title

      Cancer Lett 219

      Pages: 105-112

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Downregulation of apoptosis revealed by laser microdissection and cDNA microarray analysis of related genes in rat liver preneoplastic lesions2005

    • Author(s)
      Ogawa, K., Asamoto, M., Suzuki, S., Tsujimura, K., Shirai, T
    • Journal Title

      Med. Mol. Morphol 38

      Pages: 23-29

    • NAID

      10018374411

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Harman and norharman suppressed but NaNO2 enhanced the development of preneoplastic liver cell foci in 2-amino-3, 8-dimethylimidazo[4,5-f] quinoxaline (MeIQx)-reated rats2005

    • Author(s)
      Sugiura, S., Asamoto, M., Hokaiwado, N., Hirose, M., Shirai, T
    • Journal Title

      J., Toxicol. Pathol 18

      Pages: 99-104

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Equivocal impact of transplacental and lactational exposure to a food-derived carcinogen, 2-amino-1-methyl-6-phenylimidazo[4,5-3]pyridine, on prostate and colon lesion development in F344 rats2005

    • Author(s)
      Ikeda, Y., Cho, Y. M., Takahashi, S., Tang, M., Asamoto, M., Ogawa, K., Shirai, T
    • Journal Title

      Cancer Lett 224

      Pages: 23-30

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Influence of atrazine administration and reduction of calorie intake on prostate carcinogenesis in probasin/SV4OT antigen transgenic rats2005

    • Author(s)
      Kandori, H., Suzuki, S., Asamoto, M., Murasaki, T., Tang, M., Ogawa, K., Shirai, T
    • Journal Title

      Cancer Sci 96

      Pages: 221-226

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Lack of effects of 1439 MHz electromagnetic near field exposure on the blood-brain barrier in immature and young rats2005

    • Author(s)
      Kuribayashi, M., Wang, J., Fujiwara, O., Doi, Y., Nabae, K., Tamano, S., Ogiso, T., Asamoto, M., Shirai, T
    • Journal Title

      Bioelectromagnetics 26

      Pages: 578-588

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Transgenic disruption of gap junctional intercellular communication protects against hepatotoxicity while increasing susceptibility to liver carcinogenesis in rats, in Carcinogenesis and Modification of Carcinogenesis2005

    • Author(s)
      Asamoto, M., Hokaiwado, N., Shirai, T
    • Journal Title

      Edited by T. Tanaka, H. Tsuda. Kerala, Research Signpost

      Pages: 1-7

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Tbx3 expression is related to apoptosis and cell proliferation in rat bladder both hyperplastic epithelial cells and carcinoma cells.2005

    • Author(s)
      Ito, A.
    • Journal Title

      Cancer Lett. 219

      Pages: 105-112

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Downregulation of apoptosis revealed by laser microdissection and cDNA microarray analysis of related genes in rat liver preneoplastic lesions.2005

    • Author(s)
      Ogawa, K.
    • Journal Title

      Med.Mol.Morphol. 38

      Pages: 23-29

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Harman and norharman suppressed but NaNO2 enhanced the development of preneoplastic liver cell foci in 2-amino-3,8-dimethylimidazo[4,5-fJquinoxaline (MeIQx)-treated rats.2005

    • Author(s)
      Sugiura, S.
    • Journal Title

      J.Toxicol.Pathol. 18

      Pages: 99-104

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Equivocal impact of transplacental and lactational exposure to a food-derived carcinogen, 2-amino-l-methyl-6-phenylimidazo[4,5-b]pvridine, on prostate and colon lesion development in F344 rats.2005

    • Author(s)
      Ikeda, Y.
    • Journal Title

      Cancer Lett. 224

      Pages: 23-30

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Influence of atrazine administration and reduction of calorie intake on prostate carcinogenesis in probasin/SV40 T antigen transgenic rats.2005

    • Author(s)
      Kandori, H.
    • Journal Title

      Cancer Sci. 96

      Pages: 221-226

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Lack of effects of 1439 MHz electromagnetic near field exposure on the blood-brain barrier in immature and young rats.2005

    • Author(s)
      Kuribayashi, M.
    • Journal Title

      Bioelectromagnetics. 26

      Pages: 578-588

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Transgenic disruption of gap junction enhances early but not late stage hepatocarcinogenesis in the rat.2005

    • Author(s)
      Hokaiwado, N.
    • Journal Title

      Toxicol.Pathol. 33・6

      Pages: 695-701

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Chronic exposure to a 1.439 GHz electromagnetic field used for cellular phones does not promote N-ethylnitrosourea induced central nervous system tumors in F344 rats.2005

    • Author(s)
      Shirai, T.
    • Journal Title

      Bioelectromagnetics 26

      Pages: 59-68

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Lack of combination hepatocarcinogenicity of harman, norharman and amitrole when given with NaNO_2 in the rat.2005

    • Author(s)
      Ichihara, T.
    • Journal Title

      J.Toxicol.Sci. 30

      Pages: 1-6

    • Related Report
      2005 Annual Research Report
  • [Presentation] Probasin/SV40Tag導入トランスジェニックラット(TRAP)前立腺癌由来ヌードマウス可移植性細胞株の樹立とその性質2007

    • Author(s)
      朝元 誠人
    • Organizer
      第18回前立腺がんワークショップ
    • Place of Presentation
      国立がんセンター研究所
    • Year and Date
      2007-09-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Establishment and characterization of rat prostate carcinoma cell lines derived from the Probasin/SV40Tag transgenic rat model2007

    • Author(s)
      Asamoto, M., Hokaiwado, N., Shirai, T
    • Organizer
      Proceedings of the 18th Workshop on Prostate Cancer
    • Place of Presentation
      Tokyo
    • Year and Date
      2007-09-28
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] Carcinogenesis and Modification of Carcinogenesis2005

    • Author(s)
      Asamoto, M
    • Publisher
      Kerala:Research Signpost
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] Carcinogenesis and Modification of Carcinogenesis2005

    • Author(s)
      Asamoto, M.
    • Publisher
      Kerala : Research Signpost
    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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