| Project/Area Number |
17590365
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
KATANO Harutaka National Institute of Infectious Diseases, Department of Pathology, Chief, 感染病理部, 室長 (70321867)
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| Co-Investigator(Kenkyū-buntansha) |
KANNO Takayuki National Institute of Infectious Diseases, Department of Pathology, Researcher, 研究官 (50272563)
SATA Tetsutaro National Institute of Infectious Diseases, Department of Pathology, Director, 部長 (00162397)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2005: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | primary effusion lymphoma / HHV-8 / adhesion molecule / SCID mice / proteomics / DNA microarray |
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| Research Abstract |
Primary effusion lymphoma (PEL) has an unique character that does not form solid lymphoma whereas all types of lymphoma other than PEL usually forms solid tumors in patients. Since all cases of PEL are associated with Kaposi's sarcoma-associated herpesvirus (KSHV, human herpesvirus 8, HHV-8) infection, KSHV should play an important role in the pathogenesis for PEL. To clarify the etiology of PEL, we inoculated a KSHV-associated PEL cell line into the peritoneal cavity of severe combined immunodeficiency mice. The inoculation resulted in the formation of effusion and solid lymphomas in the peritoneal cavity. Proteomics using 2-dimensional difference-gel electrophoresis and DNA microarray analyses identified 14 proteins and 105 genes, respectively, whose expressions differed significantly between effusion and solid lymphomas. Five genes were identified as genes with a similar expression profile with that of lymphocyte function-associated antigen 1, an important adhesion molecule in leukocytes. Among them, Coronin 1A, an actin-binding protein, was identified as a molecule with high expression in solid lymphoma by both DNA microarray and proteomics. KSHV-encoded lytic proteins, including viral interleukin 6, were highly expressed in effusion lymphoma compared with solid lymphoma. These data demonstrate that effusion and solid lymphomas possess distinctive gene and protein expression profiles in our mouse model, and suggest that the difference of gene and protein expression between effusion and solid lymphomas may be associated with formation of effusion lymphoma or invasive feature of solid lymphoma.
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