| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2005: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Research Abstract |
cDNA microarray analysis and comparison of the gene expression profiles between Trichinella spiralis and T. pseudospiralis infected muscle tissues were performed to reveal the molecular mechanism of the pathological changes and nurse cell formation. The candidate genes to be likely involved in the common and different pathological changes were listed. The expressions of the genes associated with satellite cell activation and proliferation, Pax7, desmin and M-cadherin, were up-regulated, suggesting their involvement in the roles of satellite cells during the formation of nurse cell. Differential expression of the genes related to cell differentiation was observed, for example, up-regulated expression of galectin and Pbxl in both Trichinella infections, up-regulated of Pax3, IFI202a, chordin 2 and S100A9, and down-regulated of Ndrg2 only in T. spiralis infection, and up-regulated of ATBF1, FoxH1, Rfx3 and Sufu, and down-regulated of Nanog only in T. pseudospiralis infection. Several gene
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s to regulate cell cycle and proliferation showed changes in expression after infection, for example, G0S2, CLU, MLF1, Id2, B-myb and cyclin A2, that are likely to play role in cell cycle reentry and arrest occurred during infection. The differential expression of some genes associated with apoptosis and transformation was identified. Further confirmation of the expression and kinetics of some selected genes by real time PCR revealed the different patterns between the two Trichinella infections. In addition, two signaling pathways, TGF-β and Notch signaling pathways, were confirmed to be involved in the differentiation and transformation of infected muscle cell. The present results indicated that complex pathological processes induced by Trichinella infection were regulated by many genes and signaling pathway, mainly related to muscle development, myogenesis, cell proliferation, differentiation and transformation, cell cycle regulation, and apoptosis. The differential expression of some genes is likely to be responding to different pathology between T spiralis and T pseudospiralis infection. Further analysis of some key ones of the listed candidate genes will help to reveal the molecular mechanism of nurse cell formation. Less
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