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Biochemical and Molecular Biological Study on Prenyltransferases of Entamoeba h stolytica

Research Project

Project/Area Number 17590379
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Parasitology (including Sanitary zoology)
Research InstitutionJikei University School of Medicine

Principal Investigator

KUMAGAI Masahiro  Jikei University School of Medicine, School of Medicine, Assistant Professor (00271304)

Co-Investigator(Kenkyū-buntansha) MAKIKA Asao  The Jikei University, School of Medicine, Associate Professor (90119850)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,010,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsEntamoeba histolytica / prenyltransferase / geranylgeranyltransferase / isoprenyl pyrophosphate synthetase / イソペンテニルニリン酸合成酵素 / ゲラニルゲラニルニリン酸合成酵素 / プレニル化酵素 / ゲラニルゲラニル化
Research Abstract

We have been studying on the prenyltransferases from Entamoeba histolytica (Eh), and have previously reported that a protein farnesyltransferase from Eh does not farnesylate most of EhRas proteins, which have C-terminal amino acid of Leu or Phe. This is different from other organisms whose Ras proteins end with Ser and Met, and were farnesylated by a protein farnesyltransferase.
In this study, we revealed that Eh protein geranylgeranyltransferase type I geranylgeranylates EhRas proteins with Phe or Leu end That is, most Ras proteins are geranylgeranylated by a protein geranylgeranyltransferase type I in Eh. Then, we cloned and sequenced the Eh protein geranylgeranytransferase type II, and confirmed Rab escort protein dependent geranylgeranylation of Rab proteins by Eh protein geranylgeranyltransferase type II.
The homology search on the Eh database (x12 reads) revealed only 3 genes coding isoprenyl pyrophosphate synthetases (IPPS) with almost same amino acid sequences.
Farnesyl pyrophosphate synthetase (FPPS) and geranylgeranyl pyrophosphate synthetase (GGPPS) from various organisms separated into 3 major clades in a phylogenetic tree. Those clade were FPPS ; GGPPS from mammals, fishes, and yeasts ; and GGPP from bacteria. The IPPS from Eh and its relative species Entamoeba disparbranched out long from a side of the clades of bacterial GGPPS, suggesting that IPPS from Eh is GGPPS which locates in a phiylogenetically distinctive position.

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (49 results)

All 2007 2006 2005

All Journal Article (14 results) (of which Peer Reviewed: 5 results) Presentation (34 results) Book (1 results)

  • [Journal Article] Difference in protein profiles of the isolates of Entamoeba histolytica and Edispar by surface-enhanced laser desorption ionization time-of-flight mass spectrometry(SELDI-TOF)ProteinChip assays2007

    • Author(s)
      Makioka A.
    • Journal Title

      Parasitology Research 102

      Pages: 103-110

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Difference in protein profiles of the isolates of Entamoeba histolytica and E. disparby surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF) ProteinChip assays2007

    • Author(s)
      Makioka, A., et. al.
    • Journal Title

      Parasitology Research,査読有 102

      Pages: 103-110

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Difference in protein profiles of the isolates of Entamoeba histolytica and E.dispar by surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF )Protein Chip assays2007

    • Author(s)
      Makioka A, Kumagai M, Kobayashi S, Takeuchi T.
    • Journal Title

      Parasitology Research 102

      Pages: 103-110

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of protein geranylgeranyltransferase I from the enteric protist Entamoeba histolytica2006

    • Author(s)
      Makioka A.
    • Journal Title

      Molecular & Biochemical Parasitology 145

      Pages: 216-225

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Effect of artificial gastrointestinal fluids on the excystation and metacystic development of Entamoeba invadens2006

    • Author(s)
      Makioka A.
    • Journal Title

      Parasitology Research 98

      Pages: 443-446

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Characterization of protein geranylgeranyltransferase I from the enteric protist Entamoeba histolytica2006

    • Author(s)
      Makioka, A., et. al.
    • Journal Title

      Molecular, Biochemical Parasitology, 査読有, 145, 2006, 216-225 145

      Pages: 216-225

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effect of artificial gastrointestinal fluids on the excystation and metacystic development of Entamoeba invadens2006

    • Author(s)
      Makioka, A., et. al.
    • Journal Title

      Parasitology Research,査読有 98

      Pages: 443-446

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Characterization of protein geranygeranyltransferase I from the enteric protist Entamoeba histolytica2006

    • Author(s)
      Makioka A, Kumagai M, Takeuchi T, Nozaki T
    • Journal Title

      Molecular & Biochemical Parasitology 145・2

      Pages: 216-225

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Effect of artificial gastrointestinal fluids on the excystation and metacystic development of Entamoeba invadens2006

    • Author(s)
      Makioka A, Kumagai M, kobayachi S, Takeuchi T
    • Journal Title

      Parasitology Research 98・5

      Pages: 443-446

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Characterization of protein geranylgeranyltransferase I from the enteric protist Entamoeba histolytica2006

    • Author(s)
      Makioka A, Kumagai M, Takeuchi T, Nozaki T.
    • Journal Title

      Mol Biochem Parasitol 145・2

      Pages: 216-225

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Effect of artificial gastrointestinal fluids on the excystation and metacystic development of Entamoeba invadens2006

    • Author(s)
      Makioka A, Kumagai M, Kobayashi S, Takeuchi T.
    • Journal Title

      Parasitol Res (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Entamoeba invadens:Cysteine protease inhibitors block excystation and metacystic development2005

    • Author(s)
      Makioka A.
    • Journal Title

      Experimental Parasitology 109

      Pages: 27-32

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Entamoeba invadens : Cysteine protease inhibitors block excystation and metacystic development2005

    • Author(s)
      Makioka, A., et. al.
    • Journal Title

      Experimental Parasitology 109

      Pages: 27-32

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Entamoeba invadens : cysteine protease inhibitors block excystation and metacystic development2005

    • Author(s)
      Makioka A, Kumagai M, Kobayashi S, Takeuchi T.
    • Journal Title

      Exp Parasitol 109・1

      Pages: 27-32

    • Related Report
      2005 Annual Research Report
  • [Presentation] システインプロテアーゼはEntamoebaの脱嚢及び発育に関与する2007

    • Author(s)
      牧岡 朝夫
    • Organizer
      第40回日本原生動物学会大会
    • Place of Presentation
      富山
    • Year and Date
      2007-11-17
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Inhibition of excystation and metacystic development of Entamoeba invadens by inhibitors of signaling molecules2007

    • Author(s)
      Makioka, A., et. al.
    • Organizer
      The 40th annual meeting of Japan Society of Protozoology
    • Place of Presentation
      Toyama
    • Year and Date
      2007-11-17
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] システインプロテアーゼはEntamobaの脱嚢及び発育に関与する2007

    • Author(s)
      牧岡 朝夫, 熊谷 正広, 小林 正規, 竹内 勤
    • Organizer
      第39回日本原生動物学会大会
    • Place of Presentation
      富山
    • Year and Date
      2007-11-17
    • Related Report
      2007 Annual Research Report
  • [Presentation] 人工胃液によるEntamoebaの脱嚢促進2007

    • Author(s)
      牧岡 朝夫
    • Organizer
      第48回日本熱帯医学会大会
    • Place of Presentation
      別府
    • Year and Date
      2007-10-12
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Enhanced excystation of Entamoeba by artificial gastric fluid2007

    • Author(s)
      Makioka, A., et. al.
    • Organizer
      The 48th annual meeting of Japanese Society of Tropical Medicine, October 12, 2007, Beppu
    • Place of Presentation
      Beppu
    • Year and Date
      2007-10-12
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 人工胃液によるEntmoebaの脱嚢促進2007

    • Author(s)
      牧岡 朝夫, 熊谷 正広, 小林 正規, 竹内 勤
    • Organizer
      第48回日本熱帯医学会大会
    • Place of Presentation
      別府
    • Year and Date
      2007-10-12
    • Related Report
      2007 Annual Research Report
  • [Presentation] Entamoebaの脱嚢・発育に及ぼす人工胃液及び人工腸液の効果2007

    • Author(s)
      牧岡 朝夫
    • Organizer
      第76回日本寄生虫学会大会
    • Place of Presentation
      大阪
    • Year and Date
      2007-03-29
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] SELDI-TOF-MS ProteinChipシステムによる赤痢アメーバおよびEntamoeba disparの株間の違い2007

    • Author(s)
      熊谷 正広
    • Organizer
      第76回日本寄生虫学会大会
    • Place of Presentation
      大阪
    • Year and Date
      2007-03-29
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effect of artificial gastrointestinal fluids on the excystation and metacystic development of Entamoeba invadens2007

    • Author(s)
      Makioka, A., et. al.
    • Organizer
      The 76th annual meeting of Japanese Society of Parasitology
    • Place of Presentation
      Osaka
    • Year and Date
      2007-03-29
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Proteomic differences in strains and isolates of Entamoeba histolytica and E. disparby SELDI-TOF-MS ProteinChip system2007

    • Author(s)
      Kumagai, M., et. al.
    • Organizer
      The 76th annual meeting of Japanese Society of Parasitology
    • Place of Presentation
      Osaka
    • Year and Date
      2007-03-29
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 情報伝達分子阻害剤によるEntamoebaの脱嚢及び発育の抑制2006

    • Author(s)
      牧岡 朝夫
    • Organizer
      第39回日本原生動物学会大会
    • Place of Presentation
      佐賀
    • Year and Date
      2006-11-19
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Inhibition of excystation and metacystic development of Entamoeba invadens by inhibitors of signaling molecules2006

    • Author(s)
      Makioka, A., et. al.
    • Organizer
      The 49th annual meeting of Japan Society of Protozoology
    • Place of Presentation
      Saga
    • Year and Date
      2006-11-19
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Entamoebaの増殖及び分化に対する各種サイトカラシンの効果の違い2006

    • Author(s)
      牧岡 朝夫
    • Organizer
      第66回日本寄生虫学会東日本支部大会
    • Place of Presentation
      東京
    • Year and Date
      2006-10-21
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Different effect of cytochalasins on the growth and differentiation of Entamoeba invadens2006

    • Author(s)
      Makioka, A., et. al.
    • Organizer
      The 66th annual meeting of the east Japan branch of Japanese Society of Parasitology
    • Place of Presentation
      Tokyo
    • Year and Date
      2006-10-21
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] システインプロテアーゼ阻害剤によるEntamoebaの脱嚢及び脱嚢後アメーバの発育の抑制2006

    • Author(s)
      牧岡 朝夫
    • Organizer
      第47回日本熱帯医学会大会
    • Place of Presentation
      長崎
    • Year and Date
      2006-10-12
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Inhibition of excystation and metacystic development of Entamoeba invadens by cysteine protease inhibitors2006

    • Author(s)
      Makioka, A., et. al.
    • Organizer
      The 47th annual meeting of Japanese Society of Tropical Medicine
    • Place of Presentation
      Nagasaki
    • Year and Date
      2006-10-12
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effect of artificial gastrointestinal fluids on the excystation and metacystic development of Entamoeba invadens2006

    • Author(s)
      Makioka A.
    • Organizer
      XI International Congress of Parasitology
    • Place of Presentation
      Glasgow
    • Year and Date
      2006-08-09
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Entamoebaの脱嚢および脱嚢後アメーバの発育へのシステインプロテアーゼの関与2006

    • Author(s)
      牧岡 朝夫
    • Organizer
      第75回日本寄生虫学会大会
    • Place of Presentation
      弘前
    • Year and Date
      2006-05-19
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Involvement of cysteine proteases in the encystations and metacystic development of Entamoeba invadens2006

    • Author(s)
      Makioka, A., et. al.
    • Organizer
      The 75th annual meeting of Japanese Society of Parasitology
    • Place of Presentation
      Hirosaki
    • Year and Date
      2006-03-19
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 赤痢アメーバRabのプレニル化を担うゲラニルゲラニル転移酵素II型の解析2005

    • Author(s)
      牧岡 朝夫
    • Organizer
      第28回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Year and Date
      2005-12-07
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Characterization of a protein geranylgerapyltransferase type-II responsible for prenylation of Rab small GTPases from Entamoeba histolytica2005

    • Author(s)
      Makioka, A., et. al.
    • Organizer
      The 28th annual meeting of the Molecular Biology Society of Japan
    • Place of Presentation
      Fukuoka
    • Year and Date
      2005-12-07
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 赤痢アメーバのプレニル転移酵素の解析2005

    • Author(s)
      熊谷 正広
    • Organizer
      第4回分子寄生虫学・マラリアフォーラム
    • Place of Presentation
      東京
    • Year and Date
      2005-11-05
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Molecular characterization of protein prenyltransferases from Entamoeba histolytica2005

    • Author(s)
      Kumagai, M., et. al.
    • Organizer
      The 4th meeting for Molecular Parasitology and Malaria
    • Year and Date
      2005-11-05
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 赤痢アメーバのゲラニルゲラニル転移酵素II型とRab escort proteinの特徴2005

    • Author(s)
      熊谷 正広
    • Organizer
      第38回日本原生動物学会大会
    • Place of Presentation
      帯広
    • Year and Date
      2005-10-15
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Molecular characterization of a protein geranylgeranyltransferase type II and a Rab escort protein from Entamoeba histolytica2005

    • Author(s)
      Kumagai, M., et. al.
    • Organizer
      The 38th annual meeting of Japan Society of Protozoology
    • Place of Presentation
      Obihiro
    • Year and Date
      2005-10-15
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Entamoebaの脱嚢・発育に関わる情報伝達分子の解析2005

    • Author(s)
      牧岡 朝夫
    • Organizer
      第46回日本熱帯医学会大会
    • Place of Presentation
      京都
    • Year and Date
      2005-10-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 赤痢アメーバのゲラニルゲラニル転移酵素II型によるRabの翻訳後脂質修飾2005

    • Author(s)
      牧岡 朝夫
    • Organizer
      第46回日本熱帯医学会大会
    • Place of Presentation
      京都
    • Year and Date
      2005-10-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Signaling molecules responsible for the encystations and metacystic development of Entamoeba invadens2005

    • Author(s)
      Makioka, A., et. al.
    • Organizer
      The 46th annual meeting of Japanese Society of Tropical Medicine
    • Place of Presentation
      Kyoto
    • Year and Date
      2005-10-14
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Posttranslational lipid modification of Rab proteins by a protein geranylgeranyltransferase typeII from Entamoeba histolytica2005

    • Author(s)
      Makioka, A., et. al.
    • Organizer
      The 46th annual meeting of Japanese Society of Tropical Medicine
    • Place of Presentation
      Kyoto
    • Year and Date
      2005-10-14
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Cysteine protease inhibitors block excystation and metacystic development of Entamoeba invadens2005

    • Author(s)
      Makioka A.
    • Organizer
      XII International Congress of Protozoology
    • Place of Presentation
      Guangzhou
    • Year and Date
      2005-07-12
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Entamoebaの脱嚢・発育への情報伝達分子プロテインキナーゼCおよびフォスファチジルイノシトール3キナーゼの関与2005

    • Author(s)
      牧岡 朝夫
    • Organizer
      第74回日本寄生虫学会大会
    • Place of Presentation
      米子
    • Year and Date
      2005-04-09
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 赤痢アメーバのゲラニルゲラニル転移酵素II型の解析2005

    • Author(s)
      熊谷 正広
    • Organizer
      第74回日本寄生虫学会大会
    • Place of Presentation
      米子
    • Year and Date
      2005-04-09
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Involvement signaling through protein kinase C and phosphatidylinositol 3-kinase in the excystation and metacystic development of Entamoeba invadens2005

    • Author(s)
      Makioka, A., et. al.
    • Organizer
      The 74th annual meeting of Japanese Society of Parasitology
    • Place of Presentation
      Yonago
    • Year and Date
      2005-04-09
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Molecular cloning and characterization of a protein geranylgeranyltransferase from Entamoeba histolytica2005

    • Author(s)
      Kumagai, M., et. al.
    • Organizer
      The 74th annual meeting of Japanese Society of Parasitology
    • Place of Presentation
      Yona go
    • Year and Date
      2005-04-09
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 蟯虫症.山口 徹ら編.今日の治療指針 2006年度版2006

    • Author(s)
      熊谷 正広
    • Total Pages
      1726
    • Publisher
      医学書院
    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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