• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis for regulatory mechanism of macrophage functions by Mycobacterium tuberculosis

Research Project

Project/Area Number 17590389
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionKyoto University

Principal Investigator

KAWAMURA Ikuo  Kyoto University Graduate School of Medicine, Associate Professor, 医学研究科, 助教授 (20214695)

Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2005: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsMycobacterium tuberculosis / caspase / necrosis / apoptosis / mitochondria / macrophage / alveolar enithelial cell / chemokine / カスパーゼ9 / Mce1A / RD1 / サイトカイン
Research Abstract

In the series of study, we investigated how Mycobacterium tuberculosis modulates cell death of macrophages and the cytokine production. A virulent H37Rv triggers necrosis of infected macrophages. It is supposed that the bacterium ultimately escapes macrophages by induction of necrosis. At the initial phase of infection, however, H37Rv avoids excessive necrosis of infected host cells through induction of caspase-9 activation. It has been shown that RD1 locus in genome of M. tuberculosis is involved in the virulence of bacteria and the necrosis-inducing ability. We found that RD1 appears to encode some components possibly playing a role in the induction of host cell necrosis by inducing damage of the mitochondrial inner membrane and causing ATP depletion. In addition, we found that M tuberculosis phagocytosed in macrophages appears to keep active metabolism inside cells and some metabolites but not structural components of bacteria trigger the production of cytokines that are important for development of Th1 cells. Furthermore, we focused on the interaction between M tuberculosis and alveolar epithelial cells because the cells play a role in the first-line of defense by producing chemokines and cytokines in the lung. Results showed that the interaction of cells with Mycobacterial mammalian cell entry protein 1A (Mce1A) that play a critical role in invasion of bacteria into cells did not induce production of chemokines. However, it may promote chemokine induction by augmenting the interaction between bacteria and epithelial cells.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (38 results)

All 2007 2006 2005

All Journal Article (38 results)

  • [Journal Article] RD1 region in mycobacterial genome is involved in the induction of necrosis in infected RAW264 cells via mitochondrial membrane damage and ATP depletion.2007

    • Author(s)
      Taijin Kaku
    • Journal Title

      FEMS Microbiology Letters (accepted)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Involvement of caspase-9 in the induction of necrosis of RAW 264 cells infected with Mycobacterium tuberculosis2007

    • Author(s)
      Ryosuke Uchiyama
    • Journal Title

      Infection and Immunity 75

      Pages: 2894-2902

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Mycobacterial mammalian cell entry protein 1A (Mce1A)-mediated adherence enhances the chemokine production by A549 alveolar epithelial cells2007

    • Author(s)
      Juthapron Kohwisattanagun
    • Journal Title

      Microbiology and Immunology 51

      Pages: 253-261

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Irreversible loss of membrane-binding activity of Listeria-derived cytolysins in non-acidic conditions : a distinct difference from allied cytolysins produced by other Gram-Positive bacteria2007

    • Author(s)
      Takamasa Nomura
    • Journal Title

      Microbiology 153

      Pages: 2250-2258

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] RD1 region in mycobacterial genome is involved in the induction of necrosis in infected RAW264 cells via mitochondrial membrane damage and ATP depletion.2007

    • Author(s)
      Taijin Kaku et al.
    • Journal Title

      FEMS Microbiology Letters accepted (accepted)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Involvement of caspase-9 in the induction of necrosis of RAW 264 cells infected with Mycobacterium tuberculosis.2007

    • Author(s)
      Ryosuke Uchiyama et al.
    • Journal Title

      Infect. Immun. 75

      Pages: 2894-2902

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Mycobacterial mammalian cell entry protein 1A (Mce1A)-mediated adherence enhances the chemokine production by A549 alveolar epithelial cells.2007

    • Author(s)
      Juthapron Kohwisattanagun et al.
    • Journal Title

      Microbiol. Immunol. 51

      Pages: 253-261

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Irreversible loss of membrane-binding activity of Listeria-derived cytolysins in non-acidic conditions : a distinct difference from allied cytolysins produced by other Gram-positive bacteria.2007

    • Author(s)
      Takamasa Nomura et al.
    • Journal Title

      Microbiol. 153

      Pages: 2250-2258

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The irreversible loss of membrane-binding activity of Listeria-derived cytolysins in non-acidic condition : A2007

    • Author(s)
      Takamasa Nomura, Ikuo Kawamura, Chikara Kohda, Hisashi Baba, Yutaka Ito, et al.
    • Journal Title

      Microbiology (accepted)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Involvement of caspase-9 in the inhibition of necrosis of RAW 264 cells infected with Mycobacterium tuberculosis.2007

    • Author(s)
      Ryosuke Uchiyama, Ikuo Kawamura, Takao Fujimura, Michiko Kawanishi
    • Journal Title

      Infection and Immunity 75 (6)(in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Mycobacterial mammalian cell entry protein 1A (Mce1A)-mediated adherence enhances the chemokine production by A549 alveolar2007

    • Author(s)
      Juthaporn Kohwiwattanagun, Ikuo Kawamura, Takao Fujimura, et al.
    • Journal Title

      Microbiology and Immunology 51 (2)

      Pages: 253-261

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Dependence of the lethal effect of pore-forming haemolysins of Gram-positive bacteria on cytolytic activity2006

    • Author(s)
      Isao Watanabe
    • Journal Title

      J. Med. Microbiology 55

      Pages: 505-510

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Listeriolysin O derived from Listeria monocytogenes inhibits the effector phase of an experimental allergic rhinitis induced by ovalbumin in mice2006

    • Author(s)
      Kazuhiro Yamamoto
    • Journal Title

      Clin. Exp. Immunol 144

      Pages: 475-484

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Modification of allergic inflammation in murine model of rhinitis by different bacterial ligands : involvement of mast cells and dendritic cells2006

    • Author(s)
      Kazuhiro Yamamoto
    • Journal Title

      Clinical Experimental Allergy

      Pages: 760-769

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Dependence of the lethal effect of pore-forming haemolysins of Gram-positive bacteria on cytolytic activity.2006

    • Author(s)
      Isao Watanabe et al.
    • Journal Title

      J. Med. Microbiol. 55

      Pages: 505-510

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Listeriolysin O derived from Listeria monocytogenes inhibits the effector phase of an experimental allergic rhinitis induced by ovalbumin in mice.2006

    • Author(s)
      Kazuhiro Yamamoto et al.
    • Journal Title

      Clin. Exp. Immunol. 144

      Pages: 475-484

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Modification of allergic inflammation in murine model of rhinitis by different bacterial ligands : involvement of mast cells and dendritic cells.2006

    • Author(s)
      Kazuhiro Yamamoto et al.
    • Journal Title

      Clin. Exp. Allergy 36

      Pages: 760-769

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Modification of allergic inflammation in murine model of rhinitis by different bacterial ligands : involvement of mast2006

    • Author(s)
      Kazuhiro Yamamoto, Ikuo Kawamura, Juichi Ito, Masao Mitsuyama
    • Journal Title

      Clinical and Experimental Allergy 36 (6)

      Pages: 760-769

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Listeriolysin 0 derived from Listeria monocytogenes inhibits the effector phase of an experimental allergic rhinitis2006

    • Author(s)
      Kazuhiro Yamamoto, Ikuo Kawamura, Takanari Tominaga, Takamasa Nomura
    • Journal Title

      Clinical and Experimental Immunology 144 (3)

      Pages: 475-484

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Dependence of the lethal effect of pore-forming haemolysins of Gram-positive bacteria on cytolytic activity2006

    • Author(s)
      Isao Watanabe, Takamasa Nomura, Takanari Tominaga, Kazuhiro
    • Journal Title

      Journal of Medical Microbiology 55 (5)

      Pages: 505-510

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Listeriolysin O derived from Listeria monocytogenes inhibits the effector phase of an experimental allergic2006

    • Author(s)
      Kazuhiro, Yamamoto
    • Journal Title

      Clin.Exp.Immunol. (accepted)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Streptomycin-dependent exhibition of cytokine-inducing activity in streptomycin-dependent Mycobacterium tuberculosis strain 18b2005

    • Author(s)
      Yutaka Fukazawa
    • Journal Title

      Infection and Immunity 73

      Pages: 7051-7055

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Listeriolysin O-induced membrane permeation mediates persistent interleukin-6 production in Caco-2 cells during Listeria monocytogenes infection in vitro.2005

    • Author(s)
      Kohsuke Tsuchiya
    • Journal Title

      Infection and Immunity 73

      Pages: 3869-3877

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Involvement of reactive oxygen intermediate in the enhanced expression of virulence-associated genes of Listeria monocytogenes inside activated macrophages.2005

    • Author(s)
      Masato Makino
    • Journal Title

      Microbiology and Immunology 49

      Pages: 805-811

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Involvement of the virulence gene products of Yersinia enterocolitica in the immune response of infected mice2005

    • Author(s)
      Osamu Takeuchi
    • Journal Title

      FEMS Immunol Med. Microbiol 45

      Pages: 321-329

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Seeligeriolysin O, a protein toxin of Listeria seeligeri, stimulates macrophage cytokine production via Toll-like receptors in a profile different from that induced by other bacterial ligands2005

    • Author(s)
      Yutaka Ito
    • Journal Title

      Int. Immunol 12

      Pages: 1597-1606

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Listeriolysin O, a cytolisin derived from Listeria monocytogenes, inhibits generation of ovalbumin-specific Th2 immune response by skewing maturation of antigen-specific T cells into Th1 cells2005

    • Author(s)
      Kazuhiro Yamamoto
    • Journal Title

      Clinical Experimantal Immunology 142

      Pages: 268-274

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Streptomycin-dependent exhibition of cytokine-inducing activity in streptomycin- dependent Mycobacterium tuberculosis strain 18b.2005

    • Author(s)
      Yutaka Fukazawa et al.
    • Journal Title

      Infect. Immun. 73

      Pages: 7051-7055

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Listeriolysin O-induced membrane permeation mediates persistent interleukin-6 production in Caco-2 cells during Listeria monocytogenes infection in vitro.2005

    • Author(s)
      Kohsuke Tsuchiya et al.
    • Journal Title

      Infect. Immun. 73

      Pages: 3869-3877

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Involvement of reactive oxygen intermediate in the enhanced expression of virulence-associated genes of Listeria monocytogenes inside activated macrophages.2005

    • Author(s)
      Masato Makino et al.
    • Journal Title

      Microbiol. Immunol. 49

      Pages: 805-811

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Listeriolysin O, a cytolisin derived from Listeria monocytogenes, inhibits generation of ovalbumin-specific Th2 immune response by skewing maturation of antigen-specific T cells into Th1 cells.2005

    • Author(s)
      Kazuhiro Yamamoto et al.
    • Journal Title

      Clin. Exp. Immunol. 142

      Pages: 268-274

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Involvement of the virulence gene products of Yersinia enterocolitica in the immune response of infected mice.2005

    • Author(s)
      Osamu Takeuchi et al.
    • Journal Title

      FEMS Immunol Med. Microbiol. 45

      Pages: 321-329

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Seeligeriolysin O, a protein toxin of Listeria seeligeri, stimulates macrophage cytokine production via Toll-like receptors in a profile different from that induced by other bacterial ligands.2005

    • Author(s)
      Yutaka Ito et al.
    • Journal Title

      Int. Immunol. 12

      Pages: 1597-1606

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Seeligeriolysin O, a protein toxin of Listeria seeligeri, stimulates macrophage cytokine production via2005

    • Author(s)
      Yutaka, Ito
    • Journal Title

      Int.Immunol. 17(12)

      Pages: 1597-1606

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Listeriolysin O, a cytolysin derived from Listeria monocytogenes, inhibits generation of ovalbumin-specific2005

    • Author(s)
      Kazuhiro, Yamamoto
    • Journal Title

      Clin.Exp.Immunol. 142(2)

      Pages: 268-274

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Streptomycin-dependent exhibition of cytokine-inducing activity in streptomycin-dependent Mycobacterium2005

    • Author(s)
      Yutaka, Fukasawa
    • Journal Title

      Infect.Immun. 73(10)

      Pages: 7051-7055

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Involvement of the virulence gene products of Yersinia enterocolitica in the immune response of infected mice.2005

    • Author(s)
      Osamu, Takeuchi
    • Journal Title

      FEMS lmmunol.Med.Microbiol. 45(2)

      Pages: 321-329

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Listeriolysin O-induced membrane permeation mediates persistent interleukin-6 production in Caco-2 cells2005

    • Author(s)
      Kohsuke, Tsuchiya
    • Journal Title

      Infect.Immun. 73(7)

      Pages: 3869-3877

    • Related Report
      2005 Annual Research Report

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi