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Mechanism of Apoptosis Induction by Bacterial Proteases and Its Implication for Bacterial Pathogenesis.

Research Project

Project/Area Number 17590395
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionKumamoto university

Principal Investigator

TAMURA Fumio  Kumamoto University, Graduate School Medical Science, Gastroenterology and Hepatology, Assistant, 大学院医学薬学研究部, 助手 (20359962)

Co-Investigator(Kenkyū-buntansha) AKAIKE Takaaki  Kumamoto university, Graduate School of Medical Science, Microbiology, Professor, 大学院医学薬学研究部, 教授 (20231798)
AKUTA Teruo  Kumamoto university, Graduate School of Medical Science, Microbiology, Assistant, 大学院医学薬学研究部, 助手 (00346975)
SASAKI Yutaka  Kumamoto university, Graduate School of Medical Science, Gastroenterology and Hepatology, Professor, 大学院医学薬学研究部, 教授 (70235282)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsBacterial Portease / Apoptosis / a2-macrogrobulin / MMP
Research Abstract

Recently it was shown that streptococcal pyrogenic exotoxin B (SpeB), a thiol protease derived from group A Streptococcus (GAS) could cause an increase in apoptotic cell death. The present study is aimed to clarify the mechanism of apoptosis induced by a variety of bacterial proteases. When human monocyte- like U937 cells were treated with SpeB, Serratiamarcescens 56kDa protease, or Pseudomonas aeruginosa elastase, all these bacterial proteases induced apoptosis in U937 cells. Serratial protease was internalized by the cells in culture as a complex form with α2-macroglobulin (a2-M) via α2-M receptor, which resulted in apoptosis. Furthermore, expressions of Bcl-2, c-lAP1 and hsp70, which were anti-apoptotic proteins, were investigated by Western blot analysis after treatment of U937 cells with serratial protease. Serratial protease degraded c-IAP1 protein more selectively than others intracellular proteins. We previously reported that some bacterial proteases could activate human MMPs, which may play important roles in tissue degeneration, and in the present study, SpeB also was confirmed activation of MMPs. We investigated whether SpeB-activatedMMPcouldprocessTNF-a andFasligandfromcellmembranetocause apoptotic cell death. SpeB activated with proMM P-9, and soluble TN F-a and Fas ligand were released from culture cells by this activated MMP-9. SpeB-dependent induction of extensive apoptosis and its related proapoptotic molecules, e.g., soluble TNF-a, Fas ligand, and MMPs, was evident in a murine model of severe GAS infections. These results suggested that bacterial proteases induce apoptotic cell death either via α2-M receptor, or via activation of MMPs.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (35 results)

All 2007 2006 2005

All Journal Article (33 results) Book (2 results)

  • [Journal Article] S-Nitrosylation of human variant albumin Liprizzi (R410C) confers potent antibacterial and cytoprotective properties.2007

    • Author(s)
      Ishima, Y. at al.
    • Journal Title

      J Pharmacol Exp Ther. 320(3)

      Pages: 967-977

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] S-Nitrosylation of human variant albumin Liprizzi (R410C) confers potent antibacterial and cytoprotective properties.2007

    • Author(s)
      Ishima, Y.et al.
    • Journal Title

      J Pharmacol Exp Ther. 320(3)

      Pages: 969-77

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Nitrative stress through formation of 8-nitroguanosine : Insights into microbial pathogenesis.2006

    • Author(s)
      Akuta, T. et al.
    • Journal Title

      Nitric Oxide 14

      Pages: 101-108

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Analysis of urinary 8-nitroguanine, a marker of nitrative nucleic acid damage,by high-performance liquid chromatography-electrochemical detection coupled with immunoaffinity purification : Association with cigarette smoking.2006

    • Author(s)
      Sawa, T. et al.
    • Journal Title

      Free Radic. Biol. Med. 40

      Pages: 711-720

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Influence of membrane fluidity on human immunodeficiency virus type 1 entry.2006

    • Author(s)
      Harada, S. et al.
    • Journal Title

      Biochem Biophys Res Commun. 329

      Pages: 480-486

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Involvement of Salmonella enterica serovar Typhi RpoS in resistance to NO-mediated host defense against serovar Typhi infection.2006

    • Author(s)
      Alam, M.S.et al.
    • Journal Title

      Microb. Pathog. 40

      Pages: 116-125

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 8-Nitroguanine, a product of nitrative DNA damage caused by reactive nitrogen species : Formation, occurrence and implications in inflammation and carcinogenesis.2006

    • Author(s)
      Ohshima, H. et al.
    • Journal Title

      Antioxidants Redox Signal. 8(5-6)

      Pages: 1033-1045

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Nitric oxide-induced downregulation of leptin production by 3T3-L1 adipocytes.2006

    • Author(s)
      Unno, Y. et al.
    • Journal Title

      Nitric Oxide 15(2)

      Pages: 125-132

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 敗血症とショックの病態2006

    • Author(s)
      田村 文雄 他
    • Journal Title

      化学療法の領域 22(3)

      Pages: 19-24

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Guanine nitration in idiopathic pulmonary fibrosis and its implication for carcinogenesis.2006

    • Author(s)
      Terasaki, Y. et al.
    • Journal Title

      Am J Respir Crit Care Med. 174(6)

      Pages: 665-673

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Helicobacter cinaedi cellulitis and bacteremia in immunocompetent hosts after orthopedic surgery.2006

    • Author(s)
      Kitamura, T. et al.
    • Journal Title

      J Clin Microbiol. 45(1)

      Pages: 31-38

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Immunization with heat shock protein 105-pulsed dendritic cells leads to tumor rejection in mice.2006

    • Author(s)
      Yokomine, K. et al.
    • Journal Title

      Biochem Biophys Res Commun. 343(1)

      Pages: 269-278

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Proprtion of de novo cancers among colorectal cancers in Japan.2006

    • Author(s)
      Goto, H. et al.
    • Journal Title

      Gastroenterology 131(1)

      Pages: 40-46

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Does oxidative stress participate in the development of hepatocellular carcinoma?2006

    • Author(s)
      Sasaki, Y.
    • Journal Title

      J Gatroenterol. 41(12)

      Pages: 1135-1148

    • NAID

      10018541147

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Nitrative stress through formation of 8- nitroguanosine : Insights into microbial pathogenesis.2006

    • Author(s)
      Akuta, T. et al.
    • Journal Title

      Nitric Oxide 14

      Pages: 101-108

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Analysis of urinary 8-nitroguanine, a marker of nitrative nucleic acid damage, by high-performance liquid chromatography-electrochemical detection coupled with immunoaffinity purification : Association with cigarette smoking.2006

    • Author(s)
      Sawa, T. et al.
    • Journal Title

      Free Radic. Biol. Med. 40

      Pages: 711-720

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Guanine nitration in idiopathic pulmonary fibrosis and its implication for carcinogenesis.2006

    • Author(s)
      Terasaki, Y.et al.
    • Journal Title

      Am J Respir Crit Care Med. 174(6)

      Pages: 665-73

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Helicobacter cinaedi cellulitis and bacteremia in immunocompetent hosts after orthopedic surgery.2006

    • Author(s)
      Kitamura, T.et al.
    • Journal Title

      J Clin Microbiol. 45(1)

      Pages: 31-8

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Immunization with heat shock protein 105-pulsed dendritic cells leads to tumor rejection in mice.2006

    • Author(s)
      Yokomine, K.et al.
    • Journal Title

      Biochem Biophys Res Commun. 343(1)

      Pages: 269-78

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Proprtion of de novo cancers among colorectal cancrs in Japan.2006

    • Author(s)
      Goto, H.et al.
    • Journal Title

      Gastroenterology 131(1)

      Pages: 40-6

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Nitrative stress through formation of 8-nitroguanosine : Insights into microbial pathogenesis.2006

    • Author(s)
      Akuta, T.et al.
    • Journal Title

      Nitric Oxide 14

      Pages: 101-108

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Analysis of urinary 8-nitroguanine, a marker of nitrative nucleic acid damage, by high-performance liquid chromatography-electrochemical detection coupled with immunoaffinity purification : Association with cigarette smoking.2006

    • Author(s)
      Sawa, T.et al.
    • Journal Title

      Free Radic.Biol.Med. 40

      Pages: 711-720

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Influence of membrane fluidity on human immunodeficiency virus type 1 entry.2006

    • Author(s)
      Harada, S.et al.
    • Journal Title

      Biochem Biophys Res Commun. 329

      Pages: 480-486

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Involvement of Salmonella enterica serovar Typhi RpoS in resistance to NO-mediated host defense against serovar Typhi infection.2006

    • Author(s)
      Alam, M.S.et al.
    • Journal Title

      Microb.Pathog. 40

      Pages: 116-125

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 8-Nitroguanine, a product of nitrative DNA damage caused by reactive nitrogen species : Formation, occurrence and implications in inflammation and carcinogenesis.2006

    • Author(s)
      Ohshima, H.et al.
    • Journal Title

      Antioxidants Redox Signal. (In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Nitric oxide-induced downregulation of leptin production by 3T3-L1 adipocytes.2006

    • Author(s)
      Unno, Y.et al.
    • Journal Title

      Nitric Oxide (In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Interleukin-1β induces death in chondrocyte-like ATDC5 cells through mitochondrial dysfunction and energy depletion in a reactive nitrogen and oxygen species-dependent manner.2005

    • Author(s)
      Yasuhara, R. et al.
    • Journal Title

      Biochem. J. 389

      Pages: 315-323

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Nitric oxide-induced nitrative stress involved in microbial pathogenesis2005

    • Author(s)
      Zaki, M.H.et al.
    • Journal Title

      J. Pharmacol. Sci. 8

      Pages: 117-129

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effect of nitric oxide in amyloid fibril formation on transthyretin-related amyloidosis.2005

    • Author(s)
      Saito, S. et al.
    • Journal Title

      Biochemistry 44

      Pages: 11122-11129

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Interleulkin-1β induces death in chondrocyte-like ATDC5 cells through mitochondria dysfunction and energy depletion in a reactive nitrogen and oxygen species-dependent manner.2005

    • Author(s)
      Yasuhara, R. et al.
    • Journal Title

      Biochem. J. 389

      Pages: 315-323

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Interleukin-1β induces death in chondrocyte-like ATDC5 cells through mitochondrial dysfunction and energy depletion in a reactive nitrogen and oxygen species-dependent manner.2005

    • Author(s)
      Yasuhara, R.et al.
    • Journal Title

      Biochem.J. 389

      Pages: 315-323

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Nitric oxide-induced nitrative stress involved in microbial pathogenesis.2005

    • Author(s)
      Zaki, M.H.et al.
    • Journal Title

      J.Pharmacol.Sci. 8

      Pages: 117-129

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Effect of nitric oxide in amyloid fibril formation on transthyretin-related amyloidosis.2005

    • Author(s)
      Saito, S.et al.
    • Journal Title

      Biochemistry 44

      Pages: 11122-11129

    • Related Report
      2005 Annual Research Report
  • [Book] 敗血症の解明と治療戦略2006

    • Author(s)
      田村 文雄, 赤池 孝章
    • Total Pages
      382
    • Publisher
      株式会社 医薬ジャーナル社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 敗血症の解明と治療戦略2006

    • Author(s)
      田村文雄, 赤池孝章
    • Total Pages
      382
    • Publisher
      株式会社医薬ジャーナル社
    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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