| Project/Area Number |
17590400
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | KEIO UNIVERSITY |
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Principal Investigator |
SANO Gen-ichiro Keio University, School of Medicine, Instructor, 医学部, 助手 (30383730)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUO Koichi Keio University, School of Medicine, Associate Professor, 医学部, 助教授 (40229422)
MATSUI Hidenori Kitasato University, Kitasato Institute, Assistant Professor, 生命科学研究所, 講師 (30219373)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2005: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Salmonella / Macrophage / Intracellular bacteria / Apoptosis / Flagella / F-actin dissociation / 細胞内寄生細菌 |
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| Research Abstract |
The intracellular parasite, Salmonella enterica serovar Typhimurium, causes a typhoid-like systemic disease in mice. Whereas the survival of Salmonella in phagocytes is well understood, little has been documented about the anti-bacterial activity of phagocytic cell and non-phagocytic cells, also exit of intracellular Salmonella from host cells. In this project, first, we focused on c-Fos : it has been recognized as a key regulator of osteoclast-macrophage lineage determination. Surprisingly, mice lacking c-Fos are susceptible to Salmonella infection (Maruyama et al. 2007). The result suggests that c-Fos also confers protective immunity against Salmonella in mice. Next, we examined osteoblast-non phagocytic cells in bone marrow-can act against Salmonella infection or not. Interestingly, osteoblasts can exhibit a subset of phenotypes reminiscent of macrophages in the course of Salmonella infection (Maruyama et al. 2006). Third, we found that some population of infected macrophages Salmonella induce "oncosis", an irreversible progression to eukaryotic cell death characterized by swelling of the entire cell body. Intracellular Salmonella move vigorously using flagella and eventually, they intermittently exit with a flagella dependent manner (manuscript in preparation).
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