Lipid composition and the synthesis system that responds to environmental change in Stapylococcus aureus.
| Project/Area Number |
17590405
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Chugokugakuen University |
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Principal Investigator |
HAYASHI Hideo Chugokugakuen University, Faculty of Contemporary Life Science, Division of Human Nutrition, Professor, 現代生活学部, 教授 (40033203)
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| Co-Investigator(Kenkyū-buntansha) |
MANABE Yoshie Chugokugakuen University, Faculty of Contemporary Life Science, Division of Human Nutrition, Research associate, 現代生活学部, 助手(助教) (60331823)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2005: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | Staphylococcus aureus / membrane phospholipids / cardiolipin / carsidiopilin synthetase / high osmotic pressure / sigma B factor / environmental stress / 膜脂質 / ゲノム / 環境応答 / ストレス / シグマ因子 / 細菌 / 脂質 / 発現制御 |
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| Research Abstract |
The lipids composition and the synthesis system in Staphylococcus aureus are changeable depending upon environmental stresses. Cardiolipin(CL), a major phospholipids component of the bacteria, has been suggested to play important role in responding to high osmotic pressure. The relative content of cardiolipin increased from 10% ( in 0.85% NaC1 containing medium) to 50%( in 10% NaC1 containing medium), while phsphatidylglycerol(PG) decreased from 65% to 40% of the total phospholipids. Lysylphosphatidylglycerol content remained constant. Cardiolipin synthesis genes(cls) was suspected to play key role in responding to high osmotic pressure. The cls was cloned and expressed in over expression system in S. aureus. It did not produce higher content of CL. The defective strain on S. aureus could not be isolated even after many trials. In E. coli, the defective or destructed cls gene was successfully isolated and it was reported that the strain showed some disorders in cell division process. The cls appears to be essential in S. aueus but further trial should be continued. Among the three sigma factors of S. aureus, sigma B (σ^a B) was suggested to be correlated with the cls expression. The over expression ofσ^B in S. aureus enhanced CL content but the defect of the gene did not affect CL synthesis. The regulatory role of σ^B in CL synthesis needs to further study. Since the destruction of lysylphosphatidylglycerol gene was reported to enhance CL synthesis, the regulatory mechanism of membrane lipid composition and the function should be more globally studied basing on the genome-net work information. In the report, more global insight into bacterial cell membrane was briefly discussed in terms of the molecular structure, function and morphogenesis.
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Report
(3 results)
Research Products
(8 results)
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[Book] ブラック微生物学2006
Author(s)
監訳:林英生, 岩本愛吉, 神谷茂, 高橋秀実
Total Pages
909
Publisher
丸善
Description
「研究成果報告書概要(和文)」より
Related Report
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