Studies on the behavior of hantavirus envelope glycoprotein in adsorption and invasion to target cells.
| Project/Area Number |
17590410
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
MORIMATSU Kumiko Hokkaido University, Graduate School of Medicine, Assistant Professor, 大学院医学研究科, 助手 (90220722)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2005: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | HFRS / Zoonosis / Rodents / Receptor / Fusion / Neutralization / Vaccine / Integrin / ハンタウイルス / 外皮蛋白 / エンベロープ / 膜蛋白 / 糖タンパク |
|---|
| Research Abstract |
Hantaviruses are causative agents for rodent-borne viral zoonosis, HFRS and HPS. Envelope glycoproteins G1 and G2 induce neutralizing antibodies. Monoclonal antibodies possessing neutralizing activities showed fusion inhibition activities. This means vaccine target epitope for hantavirus is a fusion responsible region in envelope protein. I studied the role of envelope glycoproteins in establishment of infection in cells. At the first, we found highly sensitive cell lines (A549, BHK and VeroE6 cells) and non-permissive cell lines (MDBK) by using pseudotype VSV screening. AlphaVbeta3 integrin was reported as candidate of the cellular receptor for hantavirus entry. Therefore, we tried to induce alphaVbeta3 into MDBK. I could not detect change of infectivity in that cells. However, transfection efficiency of plasmid constract to MDBK cells were not enough to evaluate the effect of molecules. Otherwise, anti-beta3 integrin antibodies were not inhibit cellular entry of hantavirus in permissive cells found in this study. So that this molecules was considered not to be major receptor molecules in these cells. Next, I screened bindings of glycolipids from permissive cell line Vero E6 cells against hantavirus glycoprotein. Any significant binding was not detected between glycolipids and envelope proteins. These results suggested that the cellular receptor of hantavirus must be protein molecules except alphaVbeta3 integrin. Finally, a clone 5 of MDBK cells was established as relative high DNA induction and non-permissive line. And cDNA libraly from permissive cell line A5449 was produced. This study established the basis for analysis of interaction between hantavirus envelope glycoproteins and cellular molecules.
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Report
(3 results)
Research Products
(17 results)
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[Journal Article] Development of serological assays for Thottapalayam virus, an insectivore-borne Hantavirus2007
Author(s)
Okumura, M., Yoshimatsu, K., Kumperasart, S., Nakamura, I., Ogino, M., Taruishi, M., Sungdee, A, Pattamadilok, S., Ibrahim, I.N.他4名
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Journal Title
Clin Vaccine Immunol 14
Pages: 173-181
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] Analysis of the immune response of Hantaan virus nucleocapsid protein-specific CD8+ T cells in mice2007
Author(s)
Taruishi, M, Yoshimatsu, K, Araki, K, Okumura, M, Nakamura, I, Kajino, K, Arikawa. J
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Journal Title
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] Arikawa J Development of serological assays for Thottapalayam virus, an insectivore-borne Hantavirus.2007
Author(s)
Okumura M, Yoshimatsu K, Kumperasart S, Nakamura I, Ogino M, Taruishi M, Sungdee A, Pattamadilok S, Ibrahim IN, Erlina S, Agui T, Yanagihara R
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Journal Title
Clin Vaccine Immunol 14
Pages: 173-181
Description
「研究成果報告書概要(欧文)」より
Related Report
-
[Journal Article] Development of serological assays for Thottapalayam virus, an insectivore-borne Hantavirus2007
Author(s)
Okumura, M., Yoshimatsu, K., Kumperasart, S., Nakamura, I., Ogino, M., Taruishi, M., Sungdee, A, Pattamadilok, S., Ibrahim, I.N., Erlina, S., Agui, T., Yanagihara, R., Arilkawa, J
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Journal Title
Clin Vaccine Immunol 14
Pages: 173-181
Related Report
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[Journal Article] Analysis of the immune response of Hantaan virus nucleocapsid protein-specific CD8+ T cells in mice2007
Author(s)
Taruishi, M., Yoshimatsu, K., Araki, K., Okumura, M., Nakamura, I., Kajino, K., Arikawa, J
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Journal Title
Related Report
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[Journal Article] Geographical distribution of hantaviruses in Thailand and potential human health significance of Thailand virus2006
Author(s)
Pattamadilok S, Lee BH, Kumperasart S, Yoshimatsu K, Okumura M, Nakamura I, Araki K, Khoprasert Y, Dangsupa P, Panlar P, Jandrig B, Kruger DH, Klempa B, Jakel T, Schmidt J, Ulrich R, Kariwa H, Arikawa J
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Journal Title
Am J Trop Med Hyg 75
Pages: 994-1002
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Soochong virus : an antigenically and genetically distinct hantavirus isolated from Apodemus peninsulae in Korea.2006
Author(s)
Back LJ, Kariwa H, Lokugamage K, Yoshimatsu K, Arikawa J, Takashima I, Kang JI, Moon SS, Chung SY, Kim EJ, Kang HJ, Song KJ, Klein TA, Yanagihara R, Song JW
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Journal Title
J Med Virol 78
Pages: 290-297
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Nucleocapsid protein of cell culture-adapted Seoul virus strain 80-39 : analysis of its encoding sequence, expression in yeast and immuno-reactivity.2005
Author(s)
Schmidt J, Jandrig B, Klempa B, Yoshimatsu K, Arikawa J, Meisel H, Niedrig M, Pitra C, Kruger DH, Ulrich R
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Journal Title
Virus Genes 30
Pages: 37-48
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] Nucleocapsid protein of cell culture-adapted Seoul virus strain 80-39:analysis of its encoding sequence, expression in yeast and immuno-reactivity.2005
Author(s)
Schmidt J, Jandrig B, Klempa B, Yoshimatsu K, Arikawa J, Meisel H, Niedrig M, Pitra C, Kruger DH, Ulrich R
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Journal Title
Virus Genes 30
Pages: 37-48
Related Report
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