| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥2,300,000 (Direct Cost: ¥2,300,000)
|
|---|
| Research Abstract |
Characteristics of herpes simplex virus type 1 (abbreviated HSV-1) are as follows:
(1) HSV-lis master herpesvirus and basic research of HSV-1 is performed at advanced level.
(2) HSV-1, which is the important human pathogene and infects 80-90% of human population, can cause severe diseases, e.g., herpes encephalitis and herpetic keratitis (the main cause of blindness).
Genome DNA maturation of HSV-1 is assumed to be done as follows:
(1) a sequence, which is the site for DNA maturation (trans-acting element), is cleaved after HSV-1 DNA replication, and virus DNA is packaged into virus particle (maturation of DNA genome).
(2) More than 10 HSV-1 genes and TAF11250 cellular gene are known to be required for DNA maturation (trans-acting elements).
<Summary of research>
The a sequence play an important role in DNA maturation of HSV-1: however, DNA sequences of the a sequence differ largely between HSV-1 isolates and it is often difficult to know effects of each element of the a sequence on the DNA m
… More
aturation: while these differences can be used as means for extracting essential regions of functions of the a sequence. Therefore, following experiments were performed in this study to reveal consensus sequences of the a sequence.
(1) Selection of analyzed HSV-1 strains: we have information concerning DNA polymorphisms of several hundred HSV-1 strains. Thirty HSV-1 strains were selected from these strains, considering diversity in DNA polymorphisms.
(2) Extraction of HSV-1 DNA: selected HSV-1 strains were propagated in cultured cells and HSV-1 DNA was extracted.
(3) Molecular cloning of the a sequences of HSV-1 strains: extracted HSV-1 DNA was subjected to electrophoresis after digestion with restriction endonuclease; DNA fragments containing the a sequence were recovered from the gel of electrophoresis, purified, inserted into cloning vector, and molecularly cloned.
(4) Determination of sequences of the a sequences: DNA sequences of molecularly cloned a sequences were determined.
(5) Existence of the a sequence, which have arrangements of elements and sequences differing from the a sequences reported previously, was revealed. Less
|
