| Project/Area Number |
17590426
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Virology
|
|---|
| Research Institution | National Institute of Infectious Diseases (2006)
Tokyo Metropolitan Organization for Medical Research (2005) |
|---|
Principal Investigator |
DATE Tomoko National Institute of Infectious Diseases, Department of Virology II, Scientist, ウイルス第二部, 研究員 (40392360)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
WAKITA Takaji National Institute of Infectious Diseases, Department of Virology II, Director, ウイルス第二部, 部長 (40280789)
MURAYAMA Asako National Institute of Infectious Diseases, Department of Virology II, Scientist, ウイルス第二部, 協力研究員 (40415534)
宮本 道子 (財)東京都医学研究機構, 研究員 (40190821)
|
|---|
| Project Period (FY) |
2005 – 2006
|
| Project Status |
Completed (Fiscal Year 2006)
|
| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
|
|---|
| Keywords | HCV / Virus / Virus replication / HCC / Chronic hepatitis / Interferon |
|---|
| Research Abstract |
We have established HCV culture system using JFH-1 strain isolated from a fulminate hepatitis patient. This system enabled us to analyze HCV life cycles in detail. In this research project, we have produced recombinant chimeric HCV using structural gene from other HCV strains and nonstructural gene from JFH-1 strain. We analyzed replication, virus production and other virological differences among chimeric viruses. We found that some chimeric viruses did not replicate and produce infectious viruses as JFH-1. However, other chimeric viruses replicated quite well and produced infectious virus into culture medium although at the lower level compared to JFH-1. Interestingly, virus titer of chimeirc viruses was recovered after several passages. We are now investigating nucleotide mutations for this adaptation. We have found one possible important mutation in envelop region which may affect antigenecity and virus production.
|
