The properties of B cell antigen receptor mediated induction signals

• Project/Area Number: 17590440
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Immunology
• Research Institution: Kawasaki Medical School (2007-2008); Kumamoto University (2005-2006)
• Principal Investigator: IGARASHI Hideya Kawasaki Medical School, 医学部, 准教授 (40291538)
• Project Period (FY): 2005 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,140,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥540,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000); Fiscal Year 2005: ¥900,000 (Direct Cost: ¥900,000)
• Keywords: B細胞 / 抗原受容体 / シグナル伝達 / 発現制御 / アポトーシス / 免疫学 / T細胞 / 胸腺細胞分化 / Double positive(DP)細胞 / Double nositive(DN)細胞 / 脱リン酸化酵素結合制御ユニット / JNK / p38 MAPK / AICD / 脱リン酸化酵素 / BTK / Caspase / Bim / 免疫寛容

Research Abstract

Bリンパ細胞は抗原受容体(BCR)を介して抗原(異物)を認識し、その異物を排除するために抗原特異的な抗体を産生する。この抗原の結合によりBCRからもたらされる信号は、Bリンパ細胞の分化段階に応じて、細胞の分化、活性化、細胞死といった様々な細胞反応を引き起こす。本研究において、新規のタンパク脱リン酸化酵素調節サブユニットであるG5PRが、リンパ細胞の生存に必須の分子であり、BCR信号伝達の調整に重要な役割を果たしていることを明らかにした。

Research Products

• [Journal Article] GANP suppresses the arginine methyltransferase PRMT5 regulating IL-4-mediated STAT6-signaling to IgE production in B cells. (2009)
  • Author(s): Hideya Igarashi, Kazuhiko Kuwahara, Mikoto Yoshida, Yan Xing, Kazuhiko Maeda, Kouichi Nakajima, Nobuo Sakaguchi.
  • Journal Title: Molecular Immunology 46 Pages: 1031-1041
  • Peer Reviewed
• [Journal Article] The immature B-cell subpopulation with low RAG1 expression is increased in the autoimmune New Zealand Black mouse. (2009)
  • Author(s): Teppei Toda, Masahiro Kitabatake, Hideya Igarashi, Nobuo Sakaguchi.
  • Journal Title: European Journal of Immunology 39 Pages: 600-611
  • Peer Reviewed
• [Journal Article] Protein phosphatase subunit G5PR that regulates the JNK-mediated apoptosis signal is essential for the survival of CD4 and CD8 double-positive thymocytes (2008)
  • Author(s): Xing Y, Wang X, Igarashi H, Kawamoto H, Sakaguchi N.
  • Journal Title: Molecular Immunology 45 Pages: 2028-2037
  • Peer Reviewed
• [Journal Article] NF-kappaB is dispensable for normal lymphocyte development in bone marrow but required for protection of progenitors from TNFalpha. (2006)
  • Author(s): Hideya Igarashi et al.
  • Journal Title: International Immunology 18 Pages: 653-669
• [Journal Article] BCR-crosslinking induces a transcription of protein phosphatase component G5PR that is required for mature B-cell survival. (2005)
  • Author(s): Huq Faisal Mhumudul Ronny et al.
  • Journal Title: Biochemical and Biophysics Research Communication 340 Pages: 338-346
• [Journal Article] Cutting edge : double-stranded DNA breaks in the IgV region gene were detected at lower frequency in affinity-maturation impeded GANP-/-mice. (2005)
  • Author(s): Yosuke Kawatani et al.
  • Journal Title: Journal of Immunology 175 Pages: 5615-5618
• [Journal Article] Protein phosphatase subunit G5PR is needed for inhibition of B cell receptor-induced apoptosis. (2005)
  • Author(s): Yan Xing et al.
  • Journal Title: Journal of Experimental Medicine 202 Pages: 707-719
• [Journal Article] Early lymphoid progenitors in mouse and man are highly sensitive to glucocorticoids. (2005)
  • Author(s): Hideya Igarashi et al.
  • Journal Title: International Immunology 17 Pages: 501-511
  • NAID: 10019359022
• [Presentation] NZB-like autoimmunity was induced in the transgenic mice of B cell survival molecule G5PR. (2008)
  • Author(s): Masahiro Kitabatake, Hideya Igarashi, Teppei Toda, Mareki Oh tsuji, Hiromichi Tsurui, Sachiko Hirose, Nobuo Sakaguchi.
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都国際会館(京都)
  • Year and Date: 2008-12-03
• [Presentation] Alteration of receptor editing activity in the B cell differentiation of NZB mouse. (2008)
  • Author(s): Teppei Toda, Masahiro Kitabatake, Hideya Igarashi, Nobuo Sakaguchi.
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都国際会館(京都)
  • Year and Date: 2008-12-03
• [Presentation] Search for cell-intrinsic abnormalities of synovial fibroblasts in rheumatoid arthritis (2007)
  • Author(s): Katsuhiko Ishihara, Hideya Igarashi, Toshiyuki Fukada, Masaaki Murakami, Toshio Hirano
  • Organizer: 第37回日本免疫学会・学術集会
  • Place of Presentation: 品川プリンスホテル、東京
  • Year and Date: 2007-11-20
• [Presentation] Role of G5PR in the development of autoimmune disease (2007)
  • Author(s): Masahiro Kitabatake, Hideya Igarashi, Teppei Toda, Mareki Ohtsuji, Hiromichi Tsurui, Sachiko Hirose, Nobuo Sakaguchi
  • Organizer: 第37回日本免疫学会・学術集会
  • Place of Presentation: 品川プリンスホテル、東京
  • Year and Date: 2007-11-20
• [Presentation] Protein phosphatase subunit G5PR regulates the JNK-mediated apoptosis signal and is required for survival of CD4^+CD8^+double-positive thymocytes (2007)
  • Author(s): Xing Yan, Wang Xiaodan, Hideya Igarashi, Hiroshi Kawamoto, Nobuo Sakaguchi
  • Organizer: 第37回日本免疫学会・学術集会
  • Place of Presentation: 品川プリンスホテル、東京
  • Year and Date: 2007-11-20
• [Presentation] Protein phosphatase subunit G5PR is required for survival of CD4+CD8+double-positive thymocytes (2007)
  • Author(s): 刑 岩、王 暁丹、五十嵐 英哉、阪口 薫雄
  • Organizer: 第66回日本癌学会総会
  • Place of Presentation: 横浜国際会議場、横浜
  • Year and Date: 2007-10-05