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Regulatory mechanisms of expression and localization of aquaporin water channels in brain microvessels

Research Project

Project/Area Number 17590473
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied pharmacology
Research InstitutionUniversity of Miyazaki

Principal Investigator

KOBAYASHI Hideyuki  University of Miyazaki, Faculty of Medicine, Associate Professor, 医学部, 助教授 (40148953)

Co-Investigator(Kenkyū-buntansha) WADA Akihiko  University of Miyazaki, Faculty of Medicine, Professor, 医学部, 教授 (30131949)
YANAGITA Toshihiko  University of Miyazaki, Faculty of Medicine, Associate, 医学部, 助手 (60295227)
YOKOO Hiroki  University of Miyazaki, Faculty of Medicine, Associate, 医学部, 助手 (30332894)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2005: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordsbrain microvessel / aquaporin / endothelial cells / brain edema / raft / glucocorticoid / agrin / regulation of expression / アストロサイト / 網膜
Research Abstract

We have studied the regulatory mechanisms of expression and localization of aquaporin (AQP) water channels in brain microvessels as molecules regulating water permeability between blood and brain, and have tried to their pathophysiological
The expression of AQP1 in cultured brain microvascular endothelial GP8 cells was increased by the treatment with dexamethasone, a glucocorticoid, by increasing transcription in a concentration-and time-dependent manner. AQP1 was concentrated in lipid microdomain rafts, suggesting that the interaction of AQP1 and lipid is important for intracellular transport of AQP1 and that the induction of AQP1 may be involved in the ameliorating effects of glucocorticoid in brain edema.
In addition, we have characterized agrin, an extracellular matrix protein, as a key molecule regulating the localization of AQP. The major isoform of agrin expressed in the brain microvessels was long N-terminal (LN) agrin, and its N-terminal contained a cluster of hydrophobic amino, a putative signal sequence for secretion. The N-terminal of LN agrin fused with green fluorescence protein was secreted into extracellular milieu. It is suggested that LN agrin expressed by the brain microvessels may regulate the localization of AQPs at the interface between brain microvessels and astrocytes.
To know role of AQP in the edema formation in the CNS, we have investigated the expression of AQP4 in the retina during endotoxin-induced uveitis in rats. The expression of AQP4 in the Muller cells facing the microvessels reduced slightly after endotoxin treatment, then returned to the control level. The expression of Kir4.1 potassium channel reduced to less than half by the endotoxin treatment, and remained at low level. The differential expression of AQP4 and Kir4.1 during endotoxin-induced uveitis implies a disturbance of water and potassium transport in the retina, which may contribute to the retinal edema during ocular inflammation.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (24 results)

All 2007 2006 2005

All Journal Article (24 results)

  • [Journal Article] Differential expression of Kir4.1 and aquaporin 4 in the retina from endotoxin-induced uveitis rat.2007

    • Author(s)
      Liu X.Q.et al.
    • Journal Title

      Molec. Vision 13

      Pages: 309-317

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Differential expression of Kir4.1 and aquaporin 4 in the retina from endotoxin-induced uveitis rat.2007

    • Author(s)
      Liu X.Q., Kobayashi H., Jin Z.B., Wada A., Nao-I N.
    • Journal Title

      Molecular Vision 13

      Pages: 309-317

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Enhancement of insulin-induced PI3K/Akt/GSK-3beta and ERK signaling by neuronal nicotinic receptor/PKC-alpha/ERK pathway : up-regulation of IRS-1/-2 mRNA and protein in adrenal chromaffin cells.2006

    • Author(s)
      Sugano T. et al.
    • Journal Title

      J. Neurochem. 98

      Pages: 20-33

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Constitutive activity of glycogen synthase kinase-3beta : Positive regulation of steady-state levels of insulin receptor substrates-1 and -2 in adrenal chromaffin cells.2006

    • Author(s)
      Nemono T. et al.
    • Journal Title

      Brain Res. 1110

      Pages: 1-12

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Induction of aquaporin 1 by dexaraethasone in lipid rafts in immortalized brain microvascular endothelial cells2006

    • Author(s)
      Kobayashi H. et al.
    • Journal Title

      Brain Res. 1123

      Pages: 12-19

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Enhancement of insulin-induced PI3K/Akt/GSK-3 beta and ERK signaling by neuronal nicotinic receptor/PKC-alpha/ERK pathway : up-regulation of IRS-1/-2 mRNA and protein in adrenal chromaffin cells.2006

    • Author(s)
      Sugano T., Yanagita T., Yokoo H., Satoh S., Kobayashi H., Wada A.
    • Journal Title

      Journal of Neurochemistry 98

      Pages: 20-33

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Constitutive activity of glycogen synthase kinase-3beta : Positive regulation of steady-state levels of insulin receptor substrates-1 and-2 in adrenal chromaffin cells.2006

    • Author(s)
      Nemoto T., Yokoo H., Satoh S., Yanagita T., Sugano T., Yoshikawa N., Maruta T., Kobayashi H., Wada A.
    • Journal Title

      Brain Research 1110

      Pages: 1-12

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Induction of aquaporin 1 by dexamethasone in lipid rafts in immortalized brain microvascular endothelial cells2006

    • Author(s)
      Kobayashi H., Yokoo H., Yanagita T., Satoh S., Kis B., Deli M., Niwa M., Wada A.
    • Journal Title

      Brain Research 1123

      Pages: 12-19

    • NAID

      120007122978

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Enhancement of insulin-induced PI3K/Akt/GSK-3beta and ERK signaling by neuronal nicotinic receptor/PKC-alpha/ERK pathway : up-regulation of IRS-1/-2 mRNA and protein in adrenal adrenal chromaffin cells.2006

    • Author(s)
      Sugano T. et al.
    • Journal Title

      J. Neurochem. 98

      Pages: 20-33

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Constitutive activity of glacogen synthase kinase-3beta : Positive regulation of steady-state levels of insulin receptor substrates-1 and -2 in adrenal chromaffin cells.2006

    • Author(s)
      Nemono T. et al.
    • Journal Title

      Brain Res. 110

      Pages: 1-12

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Induction of aquaporin 1 by dexamethasone in lipid rafts in immortalized brain microvascular endothelial cells2006

    • Author(s)
      Kobayashi H. et al.
    • Journal Title

      Brain Res 1123

      Pages: 12-19

    • NAID

      120007122978

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Insulin induces internalization of the 5-HT2A receptor expressed in HEK293 cells.2005

    • Author(s)
      Ohkura M. et al.
    • Journal Title

      Eur. J. Pharmacol. 518

      Pages: 18-21

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Relaxant effect of adrenomedullin on bovine isolated iris sphincter muscle under resting conditions.2005

    • Author(s)
      Uchikawa Y. et al.
    • Journal Title

      Clin. Exp. Pharmacol. Physiol. 32

      Pages: 675-680

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] New twist on neuronal insulin receptor signaling in health, disease, and therapeutics2005

    • Author(s)
      Wada A.et al.
    • Journal Title

      J. Pharmacol. Sci. 99

      Pages: 128-142

    • NAID

      10025730645

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Lithium : potential therapeutics against acute brain injuries and chronic neurodegenerative diseases.2005

    • Author(s)
      Wada A.et al.
    • Journal Title

      J. Pharmacol. Sci. 99

      Pages: 307-321

    • NAID

      10025731486

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] インスリン受容体シグナル伝達分子の発現調節機構2005

    • Author(s)
      横尾 宏毅 他
    • Journal Title

      日薬理誌 125

      Pages: 141-146

    • NAID

      10015568253

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Insulin induces internalization of the 5-HT2A receptor expressed in HEK293 cells.2005

    • Author(s)
      Ohkura M., Tanaka N., Kobayashi H., Wada A., Nakai J., Yamamoto R.
    • Journal Title

      European Journal of Pharmacology 518

      Pages: 18-21

    • NAID

      40015392245

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Relaxant effect of adrenomedullin on bovine isolated iris sphincter muscle under resting conditions.2005

    • Author(s)
      Uchikawa Y., Okano M., Sawada A., Asada Y., Kobayashi H., Wada A., Nao-i N., Ohkura M., Tanaka N., Yamamoto R.
    • Journal Title

      Clinical and Experimental Pharmacology and Physiology 32

      Pages: 675-680

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] New twist on neuronal insulin receptor signaling in health, disease, and therapeutics.2005

    • Author(s)
      Wada A., Yokoo H., Yanagita T., Kobayashi H.
    • Journal Title

      Journal of Pharmacological Sciences 99

      Pages: 128-143

    • NAID

      10025730645

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Insulin induces internalization of the 5-HT2A receptor expressed in HEK293 cells.2005

    • Author(s)
      Ohkura M. et al.
    • Journal Title

      Eur.J.Pharmacol. 518

      Pages: 18-21

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Relaxant effect of adrenomedullin on bovine isolated iris sphincter muscle under resting conditions.2005

    • Author(s)
      Uchikawa Y. et al.
    • Journal Title

      Clin.Exp.Pharmacol.Physiol. 32

      Pages: 675-680

    • Related Report
      2005 Annual Research Report
  • [Journal Article] New twist on neuronal insulin receptor signaling in health, disease, and therapeutics2005

    • Author(s)
      Wada A.et al.
    • Journal Title

      J.Pharmacol.Sci. 99

      Pages: 128-143

    • NAID

      10025730645

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Lithium : potential therapeutics against acute brain injuries and chronic neurodegenerative diseases.2005

    • Author(s)
      Wada A.et al.
    • Journal Title

      J.Pharmacol.Sci. 99

      Pages: 307-321

    • NAID

      10025731486

    • Related Report
      2005 Annual Research Report
  • [Journal Article] インスリン受容体シグナル伝達分子の発現調節機構2005

    • Author(s)
      横尾 宏毅, 他
    • Journal Title

      日薬理誌 125

      Pages: 141-146

    • NAID

      10015568253

    • Related Report
      2005 Annual Research Report

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

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