Clinicopathological analysis of H. heilmannii-related gastric malignant lymphoma
Project/Area Number |
17590488
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Laboratory medicine
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Research Institution | Shinshu University |
Principal Investigator |
OTA Hiroyoshi Shinshu University, School of Medicine, Professor (50273107)
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Co-Investigator(Kenkyū-buntansha) |
FUJITA Kiyotaka Shinshu University, School of Medicine, Associate Professor (90313866)
SANO Kenji Shinshu University, Hospital, Assistant Professor (50205994)
NAKAYAMA Jun Shinshu University, School of Medicine, Professor (10221459)
KATSUYAMA Tsutomu Shinshu University, School of Medicine, Professor (90020809)
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Project Period (FY) |
2005 – 2007
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Project Status |
Completed (Fiscal Year 2007)
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Budget Amount *help |
¥3,750,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
|
Keywords | gastritis / gastric malignant lymphoma / Helicobacter heilmannii / pathophysiology / Helicobacter heilmannii / MALTリンパ腫 |
Research Abstract |
1. We have established persistent Helicobacter heilmannii (H. heilmannii) infection in BALB/c mice. Mice were sacrificed at 8, 26 and 56 weeks after inoculation of homogenate of gastric mucosa of H. heilmannii -infected mouse, which had been established by inoculation with homogenate of gastric biopsy from the H. heilmannii -infected patient. Sections prepared from stomachs immediately fixed in 20% formalin were stained with hematoxylin and eosin and immunostaining with anti H. pylon antibody, and mouse CD45R/B220 or MECA-79. H. heilmannii infection induced in the Mongolian gerbil a chronic active gastritis, in which a slight mucosal infiltration of neutrophils on a background of predominant chronic inflammation with lymphoid follicles, Lymphoepithelial lesion (LEL) and high endothelial venule (HEV) became detectable 26 weeks after the inoculation and continued up to 56 weeks in fundic mucosa. H. heilmannii were colonized in clusters in deep in the gastric pits and pyloric glands, and did not attach to gastric epithelial cells. The mice model is a useful model for the study of H. heilmannii infection. 2. 'H. heilmannii' has been classified into types I and 2. Despite the inability of its cultivation, phylogenetic analysis of the 16S rRNA and urease genes has disclosed the occurrence of the types of 'H. heilmannii'. Prior to the examination, mice were successfully infected with the 'H. heilmannii' strains derived from the 7 patients from different district in Japan. The genomic DNA was extracted from the infected mice, amplified by using the 16S rRNA and urease primers, and then sequenced. Phylogenetic analysis of the sequences of 16S rRNA and urease genes retrieved from BLAST revealed that 6 of the 7 strains studied were 'H. heilmannii' type 1, demonstrating 99% similarities of the sequences within the 6 strains tested.
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Report
(4 results)
Research Products
(56 results)
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[Journal Article] Quantitative determination of gland mucous cells-type mucin using a monoclonal antibody, HIK1083: its pathophysiological changes in human gastric juice.2007
Author(s)
Kubota S, Yamauchi K, Kumagai T, Sugano M, Kawasaki K, Tozuka M, Akamatsu T, Matsuzawa K, Sugiyama A, Kurihara M, Katsuyama T, Ota H.
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Journal Title
Clin Chim Acta 377
Pages: 61-67
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
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[Journal Article] Quantitative analysis of the effect of Helicobacter pylori on the expressions of SOX2, CDX2, MUC2, MUC5AC, MUC6, TFF1, TFF2, and TFF3 mRNAs in human gastric carcinoma cells.2007
Author(s)
Matsuda K, Yamauchi K, Matsumoto T, Sano K, Yamaoka Y, Ota H.
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Journal Title
Scand J Gastroenterol 43
Pages: 25-33
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
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[Journal Article] Quantitative determination of gland mucous cells-type mucin using a monoclonal antibody, HIK1083 : its pathophysiological changes in human gastric juice2007
Author(s)
Kubota, S., Yamauchi, K., Kumagai, T., Sugano, M., Kawasaki, K., Tozuka, M., Akamatsu, T., Matsuzawa, IC., Sugiyama, A., Kurihara, M., Katsuyama, T., Ota, H
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Journal Title
Clin Chim Acta 377
Pages: 61-67
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Quantitative analysis of the effect of Helicobacter pylori on the expressions of SOX2, CDX2, MUC2, MUC5AC, MUC6, TFF1, TFF2, and TFF3 mRNAs in human gastric carcinoma cells2007
Author(s)
Matsuda, K., Yamauchi, K., Matsumoto, T., Sano, K., Yamaoka, Y., Ota H
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Journal Title
Scand J Gastroenterol 43
Pages: 25-33
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] In vivo bactericidal activities of Japanese rice-fluid against H. pylori in a Mongolian gerbil model2007
Author(s)
Ishizone, S., Maruta, F., Suzuki, K., Miyagawa, S., Takeuchi, M., Kanaya, K., Oana, K., Hayama, M., Kawakami, Y., Ota, H
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Journal Title
Int J Med Sci 4
Pages: 203-208
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Co-localization of TFF2 with gland mucous cell mucin in gastric mucous cells and in extracellular mucous gel adherent to normal and damaged gastric mucosa2006
Author(s)
Ota, H., Hayama, M., Momose, M., El-Zimaity, HM., Matsuda, K., Sano, K., Maruta, F., Okumura, N., Katsuyama, T
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Journal Title
Histochem Cell Biol 126
Pages: 617-625
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Phylogenetic analysis of Helicobacter heilmannii isolates from different Japanese patients based on DNA sequences of 16S rRNA and urease genes2006
Author(s)
Matsumoto, T., Kawkubo, M., Ota, H., Shiohara, M., Kumagai, T., Yamauchi, K., Katsuyama, T., Kawakami, Y
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Journal Title
Description
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[Journal Article] In vitro bactericidal activities of Japanese rice-fluid against Helicobacter pylori strains2006
Author(s)
Kawakami, Y., Oana, K., Hayama, M., Ota, H., Takeuchi, M., Miyashita, K., Matsuzawa, T., Kanaya, K
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Journal Title
Int J Med Sci 3
Pages: 112-116
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Trefoil factor 2 in gland mucous cell mucin in the mucous gel covering normal or damaged gastric mucosa using the Mongolian gerbil model2006
Author(s)
Suzuki, K., Hayama, M., Nakamura, M., Yamauchi, K., Maruta, F., Miyagawa, S., Ota, H
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Journal Title
Scand J Gastroenterol 41
Pages: 1390-1397
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Regulation of the ASC expression in response to LPS stimulation is related to IL-8 secretion in the human intestinal mucosa.2006
Author(s)
Masumoto J, Kobayashi H, Nakamura T, Kaneko Y, Ota H, Hasegawa M, Kobayashi Y, Suzuki T, Matsuda K, Sano K, Katsuyama T, Inohara N.
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Journal Title
Biochem Biophys Res Commun 346・3
Pages: 968-973
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[Journal Article] A case of acute myelogenous leukemia with MLL-AF10 fusion caused by insertion of 5'MLL into 10p12,with concurrent 3'MLL deletion.2006
Author(s)
Matsuda K, Hidaka E, Ishida F, Yamauchi K, Makishima H, Ito T, Suzuki T, Imagawa E, Sano K, Katsuyama T, Ota H.
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Journal Title
Cancer Genet Cytogenet 171・1
Pages: 24-30
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[Journal Article] H.heilmannii infection and gastric mucosal lesions (in Japansese)2005
Author(s)
Ota, H., Matsuzawa, K., Okiyama, Y., Hosoda, Y., Kawakubo, Hidaka, K., Kawakami,. Y, Katsuyama, T
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Journal Title
Helicobacter research 9
Pages: 470-475
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Phylogenetic analysis of 'Helicobacter heilmannii'-like organisms from different Japanese patients based on DNA seauences of 16S rRNA and Urease genes2007
Author(s)
Matsumoto, T., Kawakubo, M., Ota, H., Shiohara, M., Kumagai, T., Yamauchi, K., Katsuyama, T., Kawakami, Y
Organizer
International Symposium on Helicobacter heilmannii
Place of Presentation
Tokyo
Year and Date
2007-04-25
Description
「研究成果報告書概要(欧文)」より
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