| Project/Area Number |
17590495
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | University of Miyazaki |
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Principal Investigator |
OKAYAMA Akihiko University of Miyazaki, Faculty of Medicine, Professor, 医学部, 教授 (70204047)
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| Co-Investigator(Kenkyū-buntansha) |
UMEKI Kazumi University of Miyazaki, Faculty of Medicine, Sub-chief technician, 医学部, 検査部副技師長 (70381134)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2005: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | HTLV-1 / ATL / risk factors / HTLV-1 |
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| Research Abstract |
Human T-lymphotropic virus type 1 (HTLV-1) is a causative agent of adult T-cell leukemia (ATL). However, only five percents of HTLV-1 carriers develop ATL eventually. Therefore, the risk for ATL varied from case to case. The aim of this study is to establish the methods/diagnostic tools for identifying high risk carriers. First we have analyzed 54 HTLV-1 carriers whose peripheral blood samples were obtained at least twice with 10 years interval. The proviral DNA loads were measured by real-time PCR. In 24 cases whose proviral loads were less than 1% (infected cells among peripheral blood mononuclear cells or PBMC), the levels did not change significantly from 0.28% to 0.26%. However, in 30 cases whose proviral loads were more than or equal to 1%, the levels decreased significantly from 0.28% to 0.26% (p<0.005). Thus far, it was considered that the proviral loads in HTLV 1 carriers do not change for long time. The finding in the current study did not support this concept when the proviral loads were high. This result may indicate that not all of HTLV-1 carriers with high proviral loads are high risk for developing ATL. However, the proviral loads were found to increase in the several carriers with high proviral loads. The analysis of clonality of HTLV-1 infected cells and the rearrangement in the T-cell receptor-γ (TCRγ ) gene are now undergoing. In addition, a novel malignancy-associated gene product, TSLC-1, was reported to be expressed in ATL cells by Sasaki et al. TSLC-1 is also now measured in the HTLV-1 carriers who have been followed-up more than 10 years. We expect that the combination of these novel markers will enable us to identify the HTLV-1 carriers with high risk for ATL development.
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