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Proteomics-based approach identifying autoantibodies in Behcet's disease

Research Project

Project/Area Number 17590502
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionOsaka Medical College

Principal Investigator

TAKEUCHI Toru  Osaka Medical College, Faculty of Medicine, Research Associate, 医学部, 助手 (10330078)

Co-Investigator(Kenkyū-buntansha) SHIMIZU Akira  Osaka Medical College, Faculty of Medicine, Emeritus Professor, 医学部, 名誉教授 (00028581)
NAKANISHI Toyofumi  Osaka Medical College, Faculty of Medicine, Associate Professor, 医学部, 助教授 (10247843)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2005: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsBehecet's disease / autoantibody / proteome / mass spectrometry / electrophoresis / immunoprecipitation / immunoblotting / 二次元電気泳動 / Behcet病
Research Abstract

We identified autoantigens specific for Behcet's disease by proteomics-based approaches. These approaches were the combination between electrophoresis or immunoprecipitation and mass spectrometry (MS).
To detect autoantigens for Behcet's disease, we performed one or two-dimensional electrophoresis using HEp-2 cells and western blotting. There were several spots specific for Behcet's disease, which identified by comparison with sera of normal healthy controls and disease controls.
To identify these spots of interest, the gel spots were excised and each piece of the gel spots was reduced, alkylated, and digested with trypsin. The extracted peptides were analyzed with an UltraFlex. The peptide mass fingerprint was used for protein identification from the tryptic fragment size using the Mascot Search engine based on databases. We identified two proteins, α-enolase and annexin-I, which were reported as autoantigens for Behcet's disease. We have also performed the combination between immunoprecipitation and MS for detecting autoantigens. Other proteins, which are candidate autoantigens, were detected by western blotting and immunoprecipitation, and are now under identification by MS.
The combination between Immunoblotting or immunoprecipitation and MS is a useful tool for identifying autoantigens for autoimmune diseases such as Behect's disease.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (15 results)

All 2006 2005 Other

All Journal Article (15 results)

  • [Journal Article] Detection of eight antibodies in cancer patients' sera against proteins derived from the adenocarcinoma A549 cell line using proteomics-based analysis.2006

    • Author(s)
      Nakanishi T, Takeuchi T, et al.
    • Journal Title

      J Chromatogr B 838

      Pages: 15-15

    • NAID

      10025784546

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Detection and characterization of variant and modified structures of proteins in blood and tissues by mass spectrometry.2006

    • Author(s)
      Shimizu A, Nakanishi T, et al.
    • Journal Title

      Mass Spectrom Rev 25

      Pages: 686-686

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Proteomics-based approach identifying autoantibody against peroxiredoxin VI as a novel serum marker in esophageal squamous cell carcinoma2006

    • Author(s)
      Fujita Y, Nakanishi T, et al.
    • Journal Title

      Clin Cancer Res 12

      Pages: 6415-6415

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Proteomics-based discovery of α-enolase autoantibody in sera of lung adenocarcinoma2006

    • Author(s)
      Nakanishi T, Takeuchi T, et al.
    • Journal Title

      Proceedings of 53th ASMS conference on mass spectrometry and allied topics

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Detection of eight antibodies in cancer patients' sera against proteins derived from the adenocarcinoma A549 cell line using proteomics-based analysis.2006

    • Author(s)
      Nakanishi T, Takeuchi T, Ueda K, Murao H, Shimizu A.
    • Journal Title

      J Chromatogr B 838

      Pages: 15-20

    • NAID

      10025784546

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Detection and characterization of variant and modified structures of proteins in blood and tissues by mass spectrometry.2006

    • Author(s)
      Shimizu A, Nakanishi T, Miyazaki A.
    • Journal Title

      Mass Spectrom Rev. 25

      Pages: 686-712

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Proteomics-based approach identifying autoantibody against peroxiredoxin VI as a novel serum marker in esophageal squamous cell carcinoma.2006

    • Author(s)
      Fujita Y, Nakanishi T, et al.
    • Journal Title

      Clin Cancer Res. 12

      Pages: 6415-6420

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Detection of eight antibodies in cancer patients' sera against proteins derived from the adenocarcinoma A549 cell line using proteomics-based analysis.2006

    • Author(s)
      Nakanishi T
    • Journal Title

      J Chromatogr B 838・1

      Pages: 15-20

    • NAID

      10025784546

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Proteomics-based approach identifying autoantibody against peroxiredoxin VI as a novel serum marker in esophageal suquamous cell carcinoma2006

    • Author(s)
      Fujita Y
    • Journal Title

      Clin Cancer Res 12・21

      Pages: 6415-6420

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Proteomics-based discovery of α-enolase autoantibody in sera of lung adenocarcinoma2005

    • Author(s)
      Nakanishi T, Takeuchi T, et al.
    • Journal Title

      Proceedings of 53th ASMS conference on mass spectrometry and allied topics 248(MP30)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Proteomics-based discovery of -enolase autoantibody in sera of lung adenocarcinoma.2005

    • Author(s)
      Nakanishi T
    • Journal Title

      Proceediings of 53th ASMS conference on mass spectrometry and allied topics

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 肥大型心筋症を合併した全身性エリテマトーデスの一例2005

    • Author(s)
      小谷卓矢
    • Journal Title

      日本臨床免疫学会会誌 28・6

      Pages: 413-413

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Cyclosporin-AとCyclophosphamide pulseの併用が有効であった皮膚筋炎に伴う間質性肺炎の一例2005

    • Author(s)
      小谷卓矢
    • Journal Title

      日本臨床免疫学会会誌 28・3

      Pages: 148-148

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Serum Protein Profile of Rheumatoid Arthritis Treated with Anti-TNF therapy (Infliximab).

    • Author(s)
      Takeuchi T, Nakanishi T, et al.
    • Journal Title

      J Chromatogr B (In press)

    • NAID

      10025806140

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Serum Protein Profile of Rheumatoid Arthritis Treated with Anti-TNF therapy (Infliximab).

    • Author(s)
      Takeuchi T, Nakanishi N, Tabushi Y, Hata A, Shoda T, Kotani T, Shimizu A, Takubo T, Makino S, Hanafusa T.
    • Journal Title

      J Chromatogr B (in press)

    • NAID

      10025806140

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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