| Co-Investigator(Kenkyū-buntansha) |
MORIMOTO Kanehisa Osaka University, Graduate School of Medicine, Professor (20143414)
LI Jing Nippon Medical School, 医学部, Associate Professor (50250048)
NAKAYAMA Kunio Osaka University, Graduate School of Medicine, Assistant Professor (80346197)
TODA Masahiro Osaka University, Graduate School of Medicine, Assistant Professor (90362714)
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| Budget Amount *help |
¥3,440,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Research Abstract |
This study was designed to follow lifestyles and DNA stability of peripheral blood leukocytes of about 200 healthy Japanese male employees. After three years of follow-up study, the results were summarized as follows.
During the period of the study, 202 subjects in 2005, 165 subjects in 2006 and 182 subjects in 2007 aged 20-60 years old participated the investigation with an informed consent. Each subject filled a questionnaire relating to lifestyles such as cigarette smoking, alcohol drinking, working hours and nutrition balance etc. When the questionnaires were collected two weeks later after questionnaire distribution, 5-10 ml of peripheral blood were collected. Blood biochemical measurements, comet assay and DNA extraction were then conducted. The polymorphisms of XRCC1, ALDH2, CYP1A1, GSTM1 and GSTT1 were detected. This study was approved by the Ethics Committee of Osaka University.
There were 134 subjects completed the three-year follow-up study. Their lifestyles were assessed by u
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sing Morimoto Health Practice Index (HPI), and the total average of HPI was 4.86 ± 1.36. In addition, the HPI was 4.87 ± 1.36, 4.87 ± 1.37, and 4.85 ± 1.35 for year 2005, 2006, and 2007, respectively. Statistical analysis showed that there was not a significant lifestyles change during these three years (p > 0.05). In the meantime, no statistical correlations between lifestyle change and DNA stability in the peripheral blood leukocyte as detected by comet assay was detected during the three years.
However, levels of exposure to cigarette tar, working hours and alcohol drinking frequency were demonstrated to statistically correlate with a temporary DNA damage in the peripheral blood leukocyte. In the meantime, the white cell count in smokers was significantly higher than that in the nonsmokers. ALDH2 polymorphisms were significantly associated with alcohol drinking frequencies. After categorizing alcohol drinkers into ALDH2-sufficient and ALDH2-deficient groups, the drinking frequency was negatively significantly associated with the comet parameters in the present study.
Summarily, the present study suggests that, wile the lifestyle showed an association with a temporary DNA damage as detected by comet assay, the investigated lifestyle change during the three-years failed to show a significant association with the DNA stability in peripheral blood leukocytes as detected by comet assay. Less
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