| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Research Abstract |
Effects of perinatal combined exposure to tributyltin (TBT) or 1,4-dichlorobenzene (DCB), and p, p'-DDE (DDE) were investigated in rat offspring of dams ingesting TBT or DCB, and DDE during the gestational and lactational period. Dams were divided into three groups, respectively; namely control group, TBT or DCB group and TBT or DCB+DDE group. Control group rats were fed normal rat chow, CE-2,DCB group rats were given CE-2 containing 25ppm (2 mg/kg) TBT or DCB, and TBT or DCB+DDE group rats were administered CE-2 containing 25ppm TBT or DCB and 125ppm (10 mg/kg) DDE. Dams were fed prepared feed for 42 days from gestational day 1 to postpartum day 21. Rat offspring were culled on postnatal day 2 to 4 males and females each, in order to avoid differences of growth due to feeding states. Rat offspring were fed CE-2 after weaning. No deteriorated pregnancies and reproductive outcomes were observed in treated dams. Perinatal combined exposure to TBT produced growth retardation in rat offspring and that to DCB induced thymus preservation in female offspring. However, these actions were counterbalanced by concurrent exposure to DDE. Furthermore, synergistic effects of exposure to DCB and DDE were observed and the reduction of the ovary weight was seen in this experiment, which might lead to reproductive dysfunction.
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