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Identification and prevalence of antiseptip resistance genes in enterococci

Research Project

Project/Area Number 17590521
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hygiene
Research InstitutionSapporo Medical University

Principal Investigator

KOBAYAHI Nobumichi  Sapporo Medical University, School of Medicine Department of Hygiene, Professor (80186759)

Co-Investigator(Kenkyū-buntansha) ISHINO Masaho  Sapporo Medical University, School of Medicine Department of Hygiene, Associate professor (30232325)
SUMI Ayako  Sapporo Medical University, School of Medicine Department of Hygiene, Assistant professor (10363699)
NAGASHIMA Shigeo  Sapporo Medical University, School of Medicine Department of Hygiene, School of Medicine Department of Hygiene (60433116)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsenterococcus / antiseptics / resistance gene / molecular epidemiology / multidrug efflux pump / 消毒済 / 浸淫状況 / 型別
Research Abstract

The purpose of this study is to identify antiseptic resistance genes in enterococci and clarify their epidemiological characteristics. We analyzed a total of about 1100 clinical isolates from the Sapporo Medical University Hospital obtained from 1997 to 2007, and 117 strains isolated outside this hospital. These strains were analyzed for presence of genes encoding multidrug efflux pumps, EfrA, EfrB, EmeA, and QacH, by PCR. The emeA was detected in all the E.faecalis, and efrA/efrB was found in all the isolates of E.faecalis and E.faecium. However, qacH gene was not detected in any enterococcal species. Through genetic analysis of E.faecium, at least four different kinds of genes encoding multidrug-efflux proteins were detected. Furthermore, in the whole gonome of E.faecium deposited in GenBank database, at least 80 genes encoding ABC transporters were found to be described. Enterococcal ABC transporters EfrA and EfrB are considered to belong to a same group as T LmrA of lactococcus, but different from MsrC which mediates efflux of macrolides. Further analysis on influence of sorts and numbers of drug-efflux genes on expression of antiseptic resistance in enterococci was considered to be needed in the future.

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (38 results)

All 2008 2007 2006 2005 2000

All Journal Article (15 results) (of which Peer Reviewed: 5 results) Presentation (21 results) Book (2 results)

  • [Journal Article] Diversity of staphylocoagulase and identification of novel variants of staphylocoagulase gene in Staphylococcus aureus2008

    • Author(s)
      Kinoshita M, et. al.
    • Journal Title

      Microbiology and Immunology (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Diversity of staphylocoagulase and identification of novel variants of staphylocoagulase gene in Staphylococcus aureus2008

    • Author(s)
      Kinoshita, M., Kobayashi, N., Nagashima, S., Ishino, M., Otokozawa, S., Mise, K., Sumi, A., Tsutsumi, H., Uehara, N., Watanabe, N., Endo, M
    • Journal Title

      Microbiology and Immunology (in press)

    • NAID

      10027684464

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Diversity of staphylocoagulase and identification of novel variants of staphylocoagulase gene in Staphylococcus aureus.2008

    • Author(s)
      Kinoshita M, et. al.
    • Journal Title

      Microbiology and Immunology (印刷中)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prevalence and genetic diversity of aminoglycoside modifying enzymes in enterococci. In : Kobayashi N, editor. Drug Resistance of Enterococci : Epidemiology and Molecular Mechanisms. Trivandrum, India2006

    • Author(s)
      Kobayashi, N, Alam, M
    • Journal Title

      Research Signpost.

      Pages: 209-227

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Genomic diversity of low-level vancomycin resistance genes vanC-1, vanC-2, and vanC-3 in enterococci2006

    • Author(s)
      Kobayashi N, et al.
    • Journal Title

      International Journal of Infectious Diseases 10(Supp1.1)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Faecal carriage of antibiotic羊esistant enterococci in hospitalized children in Cuba2006

    • Author(s)
      Quinones D, et al.
    • Journal Title

      International Journal of Infectious Diseases 10(Supp1.1)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Analysis of the prevalence of tetracycline resistance genes in clinicalisolates of Enterococcus faecdis and Enterococus faecium in a Japanese hospital2005

    • Author(s)
      Nishimoto Y., et. al.
    • Journal Title

      Microbial Drug Resistance 11

      Pages: 146-153

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Detection of a novel aph(2") Allele(aph(2")-Ie)conferringhigh-levelgentamicin resistance and a spectinomycin resistance gene ant(9)-Ia(aad9)in clinical isolates of enterococci2005

    • Author(s)
      Alam M.M., et. al.
    • Journal Title

      Microbial Drug Resistance 11

      Pages: 239-247

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Genetic Anyalysis of mecA Homologues in Staphylococcus sciuristrains derived from mastitis in dairy cattle2005

    • Author(s)
      Rahman MT., et. al.
    • Journal Title

      Microbial Drug Resistance 11

      Pages: 205-214

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Analysis of the prevalence of tetracycline resistance genes in clinical isolates of Enterococcus faecalis and Enterococcus faecium in a Japanese hospital2005

    • Author(s)
      Nishimoto, Y., Kobayashi, N., Alam, MM., Ishino, M., Uehara, N., Watanabe, N
    • Journal Title

      Microb Drug Resist 11

      Pages: 146-153

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Detection of a novel aph(2") allele (aph(2")-Ie) conferring high-level gentamicin resistance and a spectinomycin resistance gene ant(9)-Ia (aad9) in clinical isolates of enterococci2005

    • Author(s)
      Alam, MM, Kobayashi, N., Ishino, M., Sumi, A., Kobayashi, K., Uehara, N., Watanabe, N
    • Journal Title

      Microb Drug Resist 11

      Pages: 239-247

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Genetic Anyalysis of mecA Homologues in Staphylococcus sciuri strains derived from mastitis in dairy cattle2005

    • Author(s)
      Rahman, MT., Kobayashi, N., Alam, MM, Ishino, M
    • Journal Title

      Microb Drug Resist 11

      Pages: 205-214

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Analysis of the prevalence of tetracycline resistance genes in clinical isolates of Enterococcus faecalis and Enterococcus faecium in a Japanese hospital.2005

    • Author(s)
      Nishimoto Y, Kobayashi N, et al.
    • Journal Title

      Microbial Drug Resistance 11

      Pages: 141-153

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Detection of a Novel aph(2") Allele(aph(2")-Ie) conferring high-level gentamicin resistance and a spectinomycin resistance gene ant(9)-Ia(aad9) in clinical isolates of enterococci.2005

    • Author(s)
      Alam M, Kobayashi N, et al.
    • Journal Title

      Microbial Drug Resistance 11

      Pages: 239-247

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Genetic Analysis of mecA Homologues in Staphylococcus sciuri strains derived from mastitis in dairy cattle.2005

    • Author(s)
      Rahman MT, Kobayashi N, et al.
    • Journal Title

      Microbial Drug Resistance 11

      Pages: 205-214

    • Related Report
      2005 Annual Research Report
  • [Presentation] 腸球菌におけるバンコマイシン低度耐性遺伝子vanCの多様性.2008

    • Author(s)
      渡邊祥二郎
    • Organizer
      第81回日本細菌学会
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2008-03-26
    • Related Report
      2007 Annual Research Report
  • [Presentation] Genetic diversity of the low-level vancomycin resistance gene vanC2vanC3 and identification of a novel subtype(vanC4)in Enterococcus casselitlavus2007

    • Author(s)
      Kobayashi N, 他
    • Organizer
      8th Central Amedcan & Cadbbean Congress on Parasitology & Tropical Medicine
    • Place of Presentation
      キューバ、ハバナ
    • Year and Date
      2007-12-05
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Colonizacion fecal por enterococco resistentes y virulentos en ninos ingresados en servicios pediatricos2007

    • Author(s)
      Quinones D, 他
    • Organizer
      8th Centrcan & Caribbean Congress on Parasitology & Tropical Medicine
    • Place of Presentation
      キューバ、ハバナ
    • Year and Date
      2007-12-05
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Colonizacion fecal por enterococco resistentes y virulentos en ninos ingresados en servicios pediatrico2007

    • Author(s)
      Quinones, D., Marrero, D., Cordovez, MC, Llop, A., Kobayashi, N., del, Campo, R
    • Organizer
      8th Central American & Caribbean Congress on Parasitology & Tropical Medicine
    • Place of Presentation
      Havana, Cuba
    • Year and Date
      2007-12-05
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Genomic diversity of low-level vancomycin resistance genes vanC1 vanC2,and vanC3 in enterococci2006

    • Author(s)
      Kobayashi N, 他
    • Organizer
      10th Western Pacific Congress on Chemotherapy and Infectious Diseases
    • Place of Presentation
      福岡
    • Year and Date
      2006-12-05
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Genetic characterization of Panton-Valentine leukocidine-producing Staphylococcus aureus in Bangladesh2006

    • Author(s)
      Kobayashi N, 他
    • Organizer
      10th Western Pacific Congress on Chemotherapy and Infectious Diseases
    • Place of Presentation
      福岡
    • Year and Date
      2006-12-05
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Diversity of staphylocoagulase genes and identification of a putative novel coagulase type in Staphylococcus aureus2006

    • Author(s)
      Kobayashi N, 他
    • Organizer
      10th Western Pacific Congress on Chemotherapy and Infectious Diseases
    • Place of Presentation
      福岡
    • Year and Date
      2006-12-05
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Identification of a novel g-enotype among the low-level vancomycin resistance gene vanC-2/C-3 in Enterococcus casselnlavus2006

    • Author(s)
      Kobayashi, N., Watanabe, S., Uehara, N., Watanabe, N., Wang, YH, Hayakawa, S., Oshima, H
    • Organizer
      10th Western Pacific Congress on Chemotherapy and Infectious Diseases
    • Place of Presentation
      Fukuoka, Japan
    • Year and Date
      2006-12-05
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Genetic characterization of Panton-Valentine leukocidine-producing Staphylococcus aureus in Bangladesh2006

    • Author(s)
      Kobayashi, N., Afroz, S., Khan, MAH, Nagashima, S., Otokozawa, S., Wang, YH
    • Organizer
      10th Western Pacific Congress on Chemotherapy and Infectious Diseases
    • Place of Presentation
      Fukuoka, Japan
    • Year and Date
      2006-12-05
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Diversity of staphylocoagulase genes and identification of a putative novel coagulase type in Staphylococcus aureus2006

    • Author(s)
      Kobayashi, N., Kinoshita, M., Tsutsumi, H., Ishino, M., Nagashima, S., Mise, K., Uehara, N., Watanabe, N
    • Organizer
      10th Western Pacific Congress on Chemotherapy and Infectious Diseases
    • Place of Presentation
      Fukuoka, Japan
    • Year and Date
      2006-12-05
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Prevalence of Panton-Valentine leukocidin-producing Staphylococcus aureus in skin fections in Bangladesh2006

    • Author(s)
      Kobayashi N, 他
    • Organizer
      The 12th International Congress on Infectious Diseases
    • Place of Presentation
      ポルトガル、リスボン
    • Year and Date
      2006-06-16
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Genomic diversity of low-level vancomycin resistance genes vanC1,vanC2,and vanC3 in enterococci2006

    • Author(s)
      Kobayashi N, 他
    • Organizer
      The 12th International Congress on Infectious Diseases
    • Place of Presentation
      ポルトガル、リスボン
    • Year and Date
      2006-06-16
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Faecal carriage of antibiotic-resistant enterococci in hospitalized children in Cuba2006

    • Author(s)
      Quinones D, 他
    • Organizer
      The 12th International Congress on Infectious Diseases
    • Place of Presentation
      ポルトガル、リスボン
    • Year and Date
      2006-06-16
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Genomic diversity of low-level vancomycin resistance genes vanCl, vanC2, and vanC3 in enterococci2006

    • Author(s)
      Kobayashi, N., Quinones, D., Ishino, M., Sumi, A., Mise, K
    • Organizer
      The 12th International Congress on Infectious Diseases
    • Place of Presentation
      Lisbon, Portugal.
    • Year and Date
      2006-06-16
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] carriage of antibiotic-resistant enterococci in hospitalized children in Cuba2006

    • Author(s)
      Quinones, D., Marrero, D., Cordovez, MC., Herrera, C., Kobayashi, N., Del, Campo, R., Faecal
    • Organizer
      The 12th International Congress on Infectious Diseases
    • Place of Presentation
      Lisbon, Portugal
    • Year and Date
      2006-06-16
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Prevalence of Panton-Valentine leukocidin-producing Staphylococcus aureus in skin infections in Bangladesh.2006

    • Author(s)
      Kobayashi, N., Afroz, S., Khan, AH, Ishino, M., Sumi, A., Mise, K.
    • Organizer
      The 12th International Congress on Infectious Diseases.
    • Place of Presentation
      Lisbon, Portugal.
    • Year and Date
      2006-06-16
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Prevalence of aminoglycoside resistance genes in recent clinical is olates of enterococci in a Japanese hospital2005

    • Author(s)
      Kobayashi N, 他
    • Organizer
      The 24th International Congress of Chemotherapy
    • Place of Presentation
      フィリピン、マニラ
    • Year and Date
      2005-06-05
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Prevalence of tetracycline resistance genes in clinical isolates of enterococci in a Japanese hospital2005

    • Author(s)
      Kobayashi N, 他
    • Organizer
      The 24th International Congress of Chemotherapy
    • Place of Presentation
      フィリピン、マニラ
    • Year and Date
      2005-06-05
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Prevalence of aminoglycoside resistance genes in recent clinical isolates of enterococci in a Japanese hospital2005

    • Author(s)
      Kobayashi, N., Alam, MM, Ishino, M., Sumi, A., Mise, K., Kobayashi, K
    • Organizer
      The 24th International Congress of Chemotherapy
    • Place of Presentation
      Manila, Philippines.
    • Year and Date
      2005-06-05
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Prevalence of tetracycline resistance genes in clinical isolates of enterococci in a Japanese hospital2005

    • Author(s)
      Kobayashi, N., Alam, MM, Ishino, M., Sumi, A., Mise, K
    • Organizer
      The 24th International Congress of Chemotherapy
    • Place of Presentation
      Manila, Philippines.
    • Year and Date
      2005-06-05
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Genetic diversity of the low-level vancomycin resistance gene vanC2/vanC3 and identification of a novel subtype (vanC4) in Enterococcus casseliflavus2000

    • Author(s)
      Kobayashi, N., Quinones, D., Watanabe, N
    • Organizer
      8th Central American & Caribbean Congress on Parasitology & Tropical Medicine
    • Place of Presentation
      Havana, Cuba
    • Year and Date
      2000-12-05
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] Drug Resistance of Enterococci:Epidemiology and Molecular Mechanisms2006

    • Author(s)
      Kobayashi N, 他
    • Total Pages
      227
    • Publisher
      Research Signpost
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] Prevalence and genetic diversity of aminoglycoside modifying enzymes in enterococci. In : Drug Resistance of Enterococci : Epidemiology and Molecular Mechanisms. (Kobayashi N, editor.)2006

    • Author(s)
      Kobayashi N, Alam M.
    • Total Pages
      19
    • Publisher
      Research Signpost
    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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