| Project/Area Number |
17590521
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hygiene
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
KOBAYAHI Nobumichi Sapporo Medical University, School of Medicine Department of Hygiene, Professor (80186759)
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| Co-Investigator(Kenkyū-buntansha) |
ISHINO Masaho Sapporo Medical University, School of Medicine Department of Hygiene, Associate professor (30232325)
SUMI Ayako Sapporo Medical University, School of Medicine Department of Hygiene, Assistant professor (10363699)
NAGASHIMA Shigeo Sapporo Medical University, School of Medicine Department of Hygiene, School of Medicine Department of Hygiene (60433116)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | enterococcus / antiseptics / resistance gene / molecular epidemiology / multidrug efflux pump / 消毒済 / 浸淫状況 / 型別 |
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| Research Abstract |
The purpose of this study is to identify antiseptic resistance genes in enterococci and clarify their epidemiological characteristics. We analyzed a total of about 1100 clinical isolates from the Sapporo Medical University Hospital obtained from 1997 to 2007, and 117 strains isolated outside this hospital. These strains were analyzed for presence of genes encoding multidrug efflux pumps, EfrA, EfrB, EmeA, and QacH, by PCR. The emeA was detected in all the E.faecalis, and efrA/efrB was found in all the isolates of E.faecalis and E.faecium. However, qacH gene was not detected in any enterococcal species. Through genetic analysis of E.faecium, at least four different kinds of genes encoding multidrug-efflux proteins were detected. Furthermore, in the whole gonome of E.faecium deposited in GenBank database, at least 80 genes encoding ABC transporters were found to be described. Enterococcal ABC transporters EfrA and EfrB are considered to belong to a same group as T LmrA of lactococcus, but different from MsrC which mediates efflux of macrolides. Further analysis on influence of sorts and numbers of drug-efflux genes on expression of antiseptic resistance in enterococci was considered to be needed in the future.
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