• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Mechanisms of vanadium-induced p53 phosphorylation and its toxicological significance

Research Project

Project/Area Number 17590526
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hygiene
Research InstitutionTokyo Women's Medical University

Principal Investigator

MATSUOKA Masato  Tokyo Women's Medical University, School of Medicine, Professor (50209516)

Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,370,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsMetals / Vanadium / Cadmium / Zinc / Acrylamide / p53 / Phosphorylation / Signal transduction / 活性酸素 / パナジウム / ATM / MAPキナーゼ
Research Abstract

When A549 human pulmonary epithelial cells were exposed to sodium metavanadate (NaVO3) , the pentavalent species of vanadium, phosphorylation of p53 protein at Ser15 and accumulation of total p53 protein were found in a time (8-48 hr) - and dose (10-200 μM) -dependent manner. Among serines in p53 protein immunoprecipitated from A549 cells treated with 100 μM NaVO3 for 48 hr, only Ser15 was markedly phosphorylated. Treatment with other vanadate compounds, sodium orthovanadate (Na3VO4) and ammonium metavanadate (NH4VO3) , also induced Serl5 phosphorylation and accumulation of p53 protein. While phosphorylation of extracellular signal-regulated protein kinase (ERK) was found in cells treated with NaVO3, treatment with U0126 did not suppress Serl5 phosphorylation. On the other hand, treatment with wortmannin or caffeine, the inhibitors to phosphatidylinositol 3-kinase related kinases (PIKKs) , suppressed NaVO3-induced Ser15 phosphorylation of p53 protein. The silencing of ataxia t … More elangiectasia mutated (ATM) expression using short-interference RNA resulted in the marked suppression of Ser15 phosphorylation in A549 cells exposed to NaVO3. However, treatment with antioxidants such as catalase and N-acetylcysteine did not suppress NaVO3-induced Ser15 phosphorylation. Transcriptional activation of p53 and DNA fragmentation in A549 cells treated with NaVO3 were suppressed only slightly by S15A mutation, suggesting that Ser15 phosphorylation is not essential for these responses. The present results showed that vanadate induces the phosphorylation of p53 at Ser15 depending on ATM, one of the members of PIKK family, in A549 cells. In addition, similar Ser15 phosphorylation of p53 protein was found in SH-SY5Y cells exposed to acrylamide and A549 cells exposed to zinc. In renal proximal tubular cells exposed to cadmium, signal transducers and activators of transcription (Stats) , a family of latent cytoplasmic transcription factors, were found to be phosphorylated at Ser727. Less

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (42 results)

All 2008 2007 2006 2005 Other

All Journal Article (29 results) (of which Peer Reviewed: 8 results) Presentation (13 results)

  • [Journal Article] Suppression of zinc-induced p53 phosphorylation and p21 expression by wortrnannin in A549 human pulmonary epithelial cells2008

    • Author(s)
      Junko Nakagawa
    • Journal Title

      Environmental Toxicology and Pharmacology 26

      Pages: 109-112

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Suppression of zinc-induced p53 phosphorylation and p21 expression by wortmannin in A549 human pulmonary epithelial cells2008

    • Author(s)
      Junko, Nakagawa
    • Journal Title

      Environmental Toxicology and Pharmacology, (peer reviewed) 26

      Pages: 109-112

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Phosphorylation of p53 at serine 15 in A549 pulmonary epithelial cells exposed to vanadate:involvement of ATM pathway2007

    • Author(s)
      Katsura Suzuki
    • Journal Title

      Toxicology and Applied Pharmacology 220

      Pages: 83-91

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Phosphorylation of Stats at Ser727 in renal proximal tubulaz epithelial cells exposed to cadmium2007

    • Author(s)
      Junko Nakagawa
    • Journal Title

      Environmental Toxicology and Pharmacology 24

      Pages: 252-259

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 有機溶剤による神経障害-最近の知見2007

    • Author(s)
      松岡 雅人
    • Journal Title

      Brain and Nerve-神経研究の進歩 59

      Pages: 591-596

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] アクリルアミド2007

    • Author(s)
      松岡 雅人
    • Journal Title

      Clinical Neuroscience 25

      Pages: 900-901

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Phosphorylation of p53 at serine 15 in A549 pulmonary epithelial cells exposed to vanadate : involvement of ATM pathway2007

    • Author(s)
      Katsura, Suzuki
    • Journal Title

      Toxicology and Applied Pharmacology, (peer reviewed) 220

      Pages: 83-91

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Phosphorylation of Stats at Ser727 in renal proximal tubular epithelial cells exposed to cadmium2007

    • Author(s)
      Junko, Nakagawa
    • Journal Title

      Environmental Toxicology and Pharmacology, (peer reviewed) 24

      Pages: 252-259

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Neurotoxicity of organic solvents-recent findings (in Japanese)2007

    • Author(s)
      Masato, Matsuoka
    • Journal Title

      Brain and Nerve 59

      Pages: 591-596

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Acrylamide (in Japanese)2007

    • Author(s)
      Masato, Matsuoka
    • Journal Title

      Clinical Neuroscience 25

      Pages: 900-901

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Phosphorylation of p53 at serine 15 in A549 pulmonary epithelial cells exposed to vanadate: involvement of ATM pathway2007

    • Author(s)
      Katsura Suzuki
    • Journal Title

      Toxicology and Applied Pharmacology 220

      Pages: 83-91

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phosphorylation of Stats at Ser727 in renal proximal tubular epithelial cells exposed to cadmium2007

    • Author(s)
      Junko Nakagawa
    • Journal Title

      Environmental Toxicology and Pharmacology 24

      Pages: 252-259

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of the extracellular signal-regulated protein kinase pathway in phosphorylation of p53 protein and exerting cytotoxicity in human neuroblastoma cells(SH-SY5Y)exposed to acrylamide2006

    • Author(s)
      Takeo Okuno
    • Journal Title

      Archives of Toxicology 80

      Pages: 146-153

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Cadmium induces the expression of Grp78,an endoplasmic reticulum molecular chaperone,in LLC-PKI renal epithelial cells2006

    • Author(s)
      Fang Liu
    • Journal Title

      Environmental Health Perspectives 114

      Pages: 859-864

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Involvement of the extracellular signal-regulated protein kinase pathway in phosphorylation of p53 protein and exerting cytotoxicity in human neuroblastoma cells (SH-SY5Y) exposed to acrylamide2006

    • Author(s)
      Takeo, Okuno
    • Journal Title

      Archives of Toxicology (peer reviewed) 80

      Pages: 146-153

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Cadmium induces the expression of Grp78, an endoplasmic reticulum molecular chaperone, in LLC-PK1 renal epithelial cells2006

    • Author(s)
      Fang, Liu
    • Journal Title

      Environmental Health Perspectives, (peer reviewed) 114

      Pages: 859-864

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Involvement of the extracellular signal-regulated protein kinase pathway in phosphorylation of p53 protein and exerting cytotoxicity in human neuroblastoma cells (SH-SY5Y) exposed to acrylamide2006

    • Author(s)
      Takeo Okuno
    • Journal Title

      Archives of Toxicology 80・3

      Pages: 146-153

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Cadmium induces the expression of Grp78, an endoplasmic reticulum moleculai chaperone, in LLC-PK1 renal epithelial cells2006

    • Author(s)
      Fang Liu
    • Journal Title

      Environmental Health Perspectives 114・6

      Pages: 859-864

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Involvement of the extracellular signal-regulated protein kinase pathway in phosphorylation of p53 protein and exerting cytotoxicity in human neuroblastoma cells (SH-SY5Y) exposed to acrylamide2006

    • Author(s)
      Takeo Okuno
    • Journal Title

      Archives of Toxicology (印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Activation of c-Jun N-terminal kinase by cadmium2006

    • Author(s)
      Masato Matsuoka
    • Journal Title

      Current Topics in Biochemical Research (印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Activation of c-Jun NH2-ternunal kinase by cadmium2005

    • Author(s)
      Masato Matsuoka
    • Journal Title

      Current Topics in Biochemical Research 7

      Pages: 95-100

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effects of heavy metal exposure on the phosphorylation of p53 protein2005

    • Author(s)
      Masato Matsuoka
    • Journal Title

      The Journal of Toxicological Sciences 30(Suppl)

    • NAID

      130007003915

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] アクリルアミドによる末梢神経障害2005

    • Author(s)
      松岡 雅人
    • Journal Title

      末梢神経 16

      Pages: 55-59

    • NAID

      10019604194

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Activation of c-Jun NH2-terminal kinase by cadmium2005

    • Author(s)
      Masato, Matsuoka
    • Journal Title

      Current Topics in Biochemical Research 7

      Pages: 95-100

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effects of heavy metal exposure on the phosphorylation of p53 protein2005

    • Author(s)
      Masato, Matsuoka
    • Journal Title

      Proceedings of the 32nd Annual Meeting, The Journal of Toxicological Sciences, 30(Suppl) 30

      Pages: 110-110

    • NAID

      130007003915

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Acrylamide neuropathy (in Japanese)2005

    • Author(s)
      Masato, Matsuoka
    • Journal Title

      Peripheral Nerve 16

      Pages: 55-59

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effects of heavy metal exposure on the phosphorylation of p53 protein2005

    • Author(s)
      Masato Matsuoka
    • Journal Title

      The Journal of Toxicological Sciences 30・Supplement

    • NAID

      130007003915

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Suppression of zinc-induced p53 phosphorylation and p21 expression by wortmannin in A549 human pulmonary epithelial cells

    • Author(s)
      Junko Nakagawa
    • Journal Title

      Environmental Toxicology and Pharmacology (印刷中)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phosphorylation of p53 at serine 15 in A549 pulmonary epithelial cells exposed to vanadate : involvement of ATM pathway

    • Author(s)
      Katsura Suzuki
    • Journal Title

      Toxicology and Applied Pharmacology 印刷中

    • Related Report
      2006 Annual Research Report
  • [Presentation] カドミウムによる転写制御因子STATのSer727リン酸化2008

    • Author(s)
      中川 潤子
    • Organizer
      第78回日本衛生学会総会
    • Place of Presentation
      熊本
    • Year and Date
      2008-03-31
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Cadmium induces the phosphorylation of STATs at Ser7272008

    • Author(s)
      Junko, Nakagawa
    • Organizer
      78th Annual Meeting of the Japanese Society for Hygiene
    • Place of Presentation
      Kumamoto
    • Year and Date
      2008-03-31
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] カドミウムによる転写制御因子STATのSer727リン酸化2008

    • Author(s)
      中川 潤子
    • Organizer
      第78回日本衛生学会総会
    • Place of Presentation
      熊本市
    • Year and Date
      2008-03-31
    • Related Report
      2007 Annual Research Report
  • [Presentation] バナジウムによるA549細胞のp53蛋白リン酸化2007

    • Author(s)
      鈴木 桂
    • Organizer
      第77回日本衛生学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2007-03-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] バナジウムによるp53蛋白セリン15部位リン酸化の生物学的意義2007

    • Author(s)
      稲毛田 清
    • Organizer
      第77回日本衛生学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2007-03-28
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Phosphorylation of p53 in A549 cells exposed to vanadium2007

    • Author(s)
      Katsura, Suzuki
    • Organizer
      77th Annual Meeting of the Japanese Society for Hygiene
    • Place of Presentation
      Osaka
    • Year and Date
      2007-03-28
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Biological significance of p53 at serine 15 phosphorylation exposed to vanadium2007

    • Author(s)
      Kiyoshi, Inageda
    • Organizer
      77th Annual Meeting of the Japanese Society for Hygiene
    • Place of Presentation
      Osaka
    • Year and Date
      2007-03-28
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 重金属曝露に対する小胞体ストレス応答2006

    • Author(s)
      劉 方
    • Organizer
      第76回日本衛生学会総会
    • Place of Presentation
      宇部
    • Year and Date
      2006-03-27
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Endoplasmic reticulum stress response by heavy metal exposure2006

    • Author(s)
      Fang, Liu
    • Organizer
      76th Annual Meeting of the Japanese Society for Hygiene
    • Place of Presentation
      Ube
    • Year and Date
      2006-03-27
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] RNA干渉によるGrp78遺伝子発現抑制のカドミウム細胞毒性への影響2006

    • Author(s)
      稲毛田 清
    • Organizer
      第76回日本衛生学会総会
    • Place of Presentation
      宇部
    • Year and Date
      2006-03-26
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effects of siRNA against grp78 gene on the cytotoxicity of cadmium2006

    • Author(s)
      Kiyoshi, Inageda
    • Organizer
      76th Annual Meeting of the Japanese Society for Hygiene
    • Place of Presentation
      Ube
    • Year and Date
      2006-03-26
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 重金属暴露によるp53蛋白リン酸化2005

    • Author(s)
      松岡 雅人
    • Organizer
      第32回日本トキシコロジー学会学術年会
    • Place of Presentation
      東京
    • Year and Date
      2005-07-01
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effects of heavy metal exposure on the phosphorylation of p53 protein2005

    • Author(s)
      Masato, Matsuoka
    • Organizer
      32nd Annual Meeting of the Japanese Society of Toxicology
    • Place of Presentation
      Tokyo
    • Year and Date
      2005-07-01
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi