| Project/Area Number |
17590575
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | University of Toyama |
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Principal Investigator |
HATA Yukiko University of Toyama, Graduate School of Medicine and Pharmaceutical Science for Research, Teaching Associate, 大学院医学薬学研究部, 教務職員 (30311674)
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| Co-Investigator(Kenkyū-buntansha) |
TAKIZAWA Hisao University of Toyama, Graduate School of Medicine and Pharmaceutical Science for Research, Professor, 大学院医学薬学研究部, 教授 (90171579)
KOMINATO Yoshihiko Gunma University, Graduate School of Medicine, Professor, 大学院医学系研究科, 教授 (30205512)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | human ABO gene / antisense RNA / transcription regulation / Alu配列 / antisense遺伝子 |
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| Research Abstract |
To elucidate the molecular basis of control of the ABO gene in cell-type-specific expression, during normal cell differentiation, and in cancer cells lacking A/B antigen, the mechanisms responsible for regulation of human ABO gene expression have been studied. Recently, naturally occurring antisense transcriptions have been reported to regulate gene expression through a variety of biological mechanisms. Therefore, RNA transcribed from the opposite strand of the ABO gene was investigated.
We demonstrated the expression of antisense RNA for the human ABO gene. This novel gene was named ABOAS. The transcript was approximately 2.0 kb in length and intronless. The expression of ABOAS appeared to be correlated with ABO gene expression in various cultured cells and normal tissues. The treatment of KATOIII cells with mithramycin A repressed transcription from the ABO exon 1 promoter, while it increased the ABOAS transcript. These results suggest that ABOAS transcribed from the opposite strand of the ABO gene might be involved in the regulation of ABO gene expression.
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