Analysis of heat stroke and its application for diagnosis

• Project/Area Number: 17590576
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Shimane University (2006-2007); University of Fukui (2005)
• Principal Investigator: TAKATSUKA Hisakazu Shimane University, Faculty of Medicine, Associate Professor (40242490)
• Co-Investigator(Kenkyū-buntansha): MATSUKI Takasumi Fukai University, School of Medicine, Professor (10126617) ; IIDA Reiko Fukui University, School of Medicine, Assistant Professor (40139788) ; IHO Sumiko Fukui University, School of Medicine, Assistant Professor (80151653)
• Project Period (FY): 2005 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥3,650,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥150,000); Fiscal Year 2007: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000); Fiscal Year 2005: ¥2,100,000 (Direct Cost: ¥2,100,000)
• Keywords: Heat stroke / Cvtokine / Chemokine / RT-PCR / Forensic Pathology / Body Temperature / 筋組織 / 死後硬直 / 組織化学 / ケモカイン / 免疫組織化学

Research Abstract

(1) Establishment of mouse heat stroke model and its analysis.
We have established the mouse model of heat stroke in stead of rat. We analysis heat stroke using the method of histopathology, RT-PCR, and immunohistochemistry. We put the mouse into the high temperature cage(40℃ and more high temperature), heat stroke were occurred in the short time and inflammatory cytokines and chemokines (TNF-α, IL-1β, MIP-2 and so on) were expressed soon. But the expression level was not so high as the high temperature. It is thought that the irreversible disturbance of respiratory center and strong dehydration may be happened. In the high temperature, the dehydration and disturbance of respiratory center play important role for the severe heat stroke.
(2)Induction of inflammatory cytokines due to the short length DNA (CpG DNA) .
The short DNA from broken-down bacteria and virus as well as bacteria and virus itself induce the inflammatory reactions and sepsis, so it is important to clear the mechanism in the process of worsening the heat stroke. We analysed the process of induction of inflammatory cytokines by the short length DNA and showed that Interferon α and scavenger receptor plays important roles of its process and that disturbance of Interferon α and scavenger receptor may be improved the severe heat stroke and onset of it.
(3) Clinicopathological analysisi of severe heat stroke with acute pancreatitis.
It is not common that heat stroke complicated of acute heat stroke and its reports are rare. When we play the active sports under the sunny and high temperature day, it can induce heat stroke and circulatory disturbance of blood flow of abdominal organs. Because of the disturbance of blood flow, acute pancreatitis may be occurred. It is thought that we have to pay attention to the physical condition and water replacement carefully.

Research Products

• [Journal Article] 急性壊死性膵炎を合併した熱射病の一剖検例 (2007)
  • Author(s): 高塚尚和, ら
  • Journal Title: 法医学の実際と研究 50 Pages: 199-203
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] An autopsy case of fatalheat stroke with acute necrotizing pancreatitis (2007)
  • Author(s): Takatsuka, H., Iida, R., Tsubota, E., Fy\ujihara, J., Matsuki, T., Takeshita, H
  • Journal Title: Res Pract Forns Med 50 Pages: 199-203
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Collaborative action of NF-κB and p38 MAPK is involved in CpG DNA-induced IFN・α and chemokine production in human plasmacytoid dendritic cells. (2006)
  • Author(s): 大澤陽子, ら
  • Journal Title: Journal of Immunology 177 Pages: 4841-4852
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Collaborative action of NF-KB and p38 MAPK is involved in CpG DNA -induced IFN-a and chemokine production in human plasmacytoid dendritic cells (2006)
  • Author(s): Osawa, Y., Iho, S., Takauji, R., Takatsuka, H., Yamamoto, S., Takahashi, T., Horiguchi, S., Urasaki, Y., Matsuki, T., Fujieda, S
  • Journal Title: J Immunol 177 Pages: 4841-4852
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] NF-xB/p38 MAPK-dependent and -independent pathways are involved in CpG DNA-induced IFN-a, CXCL10, and CCL3 production in human pDC (2007)
  • Author(s): Osawa, Y., Iho, S., Takatsuka, H., Matsuki, T., Fujieda, S., Yamamoto, S
  • Organizer: International Cytokine Society Conference 2005
  • Place of Presentation: Seoul
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] NF-κB/p38 MAPK-dependent and -independent pathways are involved in CpG DNA-induced IFN-α, CXCL10, and CCL3 production in human pDC. (2005)
  • Author(s): 大澤陽子, ら
  • Organizer: International Cytokine Society Conference
  • Place of Presentation: ソウル
  • Description: 「研究成果報告書概要(和文)」より