| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2005: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Research Abstract |
DNA samples as buccal swabs collected from each 200 healthy Mongolian individuals with informed consents in Ulaanbaatar (central area), Ulaangom (western area) and Dalandzadgad (in the Gobi desert as southern area) on August, 2005, and in Undurkhaan (northeastern area), Choybalsan (eastern area) on June, 2006. DNA from total 1,000 buccal swabs in five regional populations were extracted and quantified. All of those samples were genotyped for 15 STR loci and amelogenin for gender determination using an AmpFlSTR Identifiler kit. In those samples, 97 from Ulaanbaatar, 95 from Ulaangom, 100 from Dalandzadgad, 117 from Choybalsan, and 84 from Undurkhaan male samples were also analyzed using an AmpFlSTR Yfiler kit. Allele frequency data were calculated for 15 autosomal STR markers, and three tests for Hardy-Weinberg equilibrium (HWE) were performed for each locus in each regional population. No departure from HWE at all the loci in all of those five populations was observed by all the three tests. Using published and our data for those 15 STR loci in Japanese regional populations and regional populations in East and Southeast Asia, and five regional Mongolian populations in this study, a Neighbor-Joining (NJ) tree based on DA genetic distance was constructed and a Multi-Dimensional Scaling (MDS) analysis was performed. As a result, all of the five regional Mongolian populations formed a cluster in the NJ tree, and those distributions were consistent with those geographical distributions in the MDS plot. By the haplotype analysis for 17 Y-STRs, two unique clusters for Japanese were observed.
In the present study, it is suggested that genetic differences between five regional Mongolian populations and Japanese regional populations are not smaller than those between those Mongolian populations and their geographically closer populations, and that a hypothesis where the origin of Japanese exists among Buryat in Mongolia is potentially inconsistent.
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