Construction of mass spectrum library and establishment of rapid analysis system for psychotropic drugs
| Project/Area Number |
17590584
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Showa University |
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Principal Investigator |
LEE Xiao-Pen Showa University, School of Medicine, Assistant Professor, 医学部, 講師 (90245829)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2005: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | high-performance liquid / tandem mass spectrometry (MS / MS) / MS and MS / MS spectrum libraries / direct-injection HPLC-MS / MS method / drug analysis / psychotropic drugs |
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| Research Abstract |
In the present study, we constructed of MS and MS/MS spectrum libraries, and established a procedure for the simple and rapid determination of four psychotropic drugs, phenothiazines, butyrophenones, benzodiazepines and tricyclic antidepressants, in human samples by direct-injection high-performance liquid chromatography (HPLC)/tandem mass spectrometry (MS/MS) with using a new polymer column (MSpak GF). The protein and/or macromolecule matrix compounds were first eluted from the column, while the drugs were retained on the polymer stationary phase. The analytes retained on the column were then eluted into an acetonitrile-rich mobile phase using a gradient separation technique. Both ESI and APCI have been evaluated for sensitivity and suitability for the psychotropic drugs, and that positive ion ESI gave the best results for all compounds. Quantification was made by selected reaction monitoring (SRM). The recoveries of four psychotropic drugs spiked into human and urine plasma were 80-91 % and 78-93 %, respectively. The detection limits were 0.1-10 ng/ml for plasma and urine. The data obtained from determination of diazepam and its metabolite, triazolam and amoxapine in human plasma or urine after oral administration of the drugs by volunteers are also presented. The present direct-injection HPLC-MS/MS method can be recommended for use in therapeutic monitoring, clinical toxicology and forensic toxicology.
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Report
(3 results)
Research Products
(25 results)
