| Project/Area Number |
17590602
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General internal medicine (including Psychosomatic medicine)
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| Research Institution | KEIO UNIVERSITY |
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Principal Investigator |
ISHIGE Atsushi Keio University, School of Medicine, Assistant Professor, 医学部, 講師 (20383705)
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| Co-Investigator(Kenkyū-buntansha) |
IRIE Yoshifumi Keio University, School of Medicine, Assistant Professor, 医学部, 講師 (00344606)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | stress / glucocorticoid / dexamethasone / mother / child separation stress / mice / gene expression / PCR / kampo medicine |
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| Research Abstract |
We examined biological influence of chronic stress from various viewpoints. In general, when the body is exposed to the same stress repeatedly, it easily acquires tolerance against the stress and shows weaker stress reactions. We have reported that these weak stress reactions are apparent, not the result of adaptation to repeated chronic stress, and suggested that different actions (i.e. abnormality in GR expression) are evoked at appearance of this apparent stress tolerance followed by different stress responses (i.e. disturbance of serotonin secretion) despite persistent exposure to chronic stress.
In the present study, we examined (i) whether the "social stress model" can induce GR expression abnormality that is believed as an organic change in depression or (ii) which sorts of other stresses can induce long term abnormality in GR expression.
Social stress
One month after exposure to the social stress for four weeks, mice were subjected to examination of motor activity and GR expressio
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n level in the hippocampus. Mice exposed to social stress showed increase of motor activity and expression of the hippocampal GR mRNA compared to control.
Abnormality in hippocampal GR expression after the "mother/child separation stress"
Offspring mice were given the mother/child separation stress for one week subsequent to birth. These offsprings at the age of ten weeks showed increase in hippocampal GR expression, however they showed insignificant change compared to control in the dexamethasone suppression test.
Behavioral abnormality caused by the separation stress
We examined influence of the separation stress on behavior on the offsprings. Behavior of the offsprings at 8 weeks was examined by the motor activity test and the elevated x-maze test. Results were not consistent between families. It was suggested that the influence of the separation stress on the offsprings can be invalidated in some families.
Conclusion
Exposure of mice to the social stress for four weeks induces long term increase of hippocampal GR expression.
It was suggested that the effects of the separation stress on behavior of offsprings are different from the dams. Increase of hippocampal GR expression continues for long term (〜10 weeks). Less
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