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Analysis of the new function of JAM-1 which is related to the differentiation of pancreatic endocrine cells

Research Project

Project/Area Number 17590616
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionThe University of Tokyo

Principal Investigator

MASHIMA Hirosato  The University of Tokyo, Faculty of Medicine, Research Associate (10261869)

Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,740,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsAR42J cells / JAM-1 / tight junction (TJ) / differentiation / pancreatic endocrine cells / Jam-1
Research Abstract

Pancreatic AR42J cells demonstrate the pluripotency in precursor cells of the gut endoderm and also provide an excellent model system to study the differentiation of the pancreas. Using the mRNA differential display technique, we identified junctional adhesion molecule-1(JAM-1), a component of the tight junction, was highly up-regulated during the differentiation of AR42J cells, although junctions were not formed. The expression level of JAM-1 showed an up-regulation in the mRNA level after 3 hours and in the protein level after 24 hours in [activin A+betacellulin]-treated AR42J cells. The expressions of its signaling molecules, PAR-3 and atypical PKCλ, also increased after the addition of activin A+betacellulin. When JAM-1 was over-expressed in [activin A+betacellulin]-treated AR42J cells, tagged-JAM-1 was observed in cytoplasm as vesicular structures and JAM-1 was colocalized with Rab3B and Rab13, members of the Rab family expressed at tight junctions. In streptozotocin-induced regenerating islets, the expression of JAM-1 was also up-regulated in the mRNA level and the protein level. JAM-1 might therefore play an important role in the differentiation of AR42J cells and the regeneration of pancreatic islets.

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (18 results)

All 2008 2007 2006 2005 Other

All Journal Article (14 results) (of which Peer Reviewed: 6 results) Presentation (4 results)

  • [Journal Article] Up-regulation of JAM-1 in AR42J cells treated with activin A and betacellulin and the diabetic regenerating islets.2008

    • Author(s)
      Yoshikumi Y, Ohno H, Suzuki J, Isshiki M, Morishita Y, Ohnishi H, Yasuda H, Omata M, Fujita T, Mashima H.
    • Journal Title

      Endocrine Journal (In press)

    • NAID

      10022609359

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Involvement of VAMP7 in Human Gastric Epithelial Cell Vacuolation Induced by Helicobacter pylori-produced VacA.2008

    • Author(s)
      Mashima H, Suzuki J, Hirayama T, Yoshikumi Y, Ohno H, Ohnishi H, Yasuda H, Fujita T, Omata M.
    • Journal Title

      Infection And Immunity (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Up-regulation of JAM-1 in AR42J cells treated with activin A and betacellulin and the diabetic regenerating islets.2008

    • Author(s)
      Yoshikumi Y, Ohno H, Suzuki J, Isshiki M, Morishita Y, Ohnishi H, Yasuda H, Omata M, Fuiita T, Mashima H.
    • Journal Title

      Endocrine Journal(掲載確定)

    • NAID

      10022609359

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of VAMP7 in Human Gastric Epithelial Cell Vacuolation Induced by Helicobacter pylori-produced VacA.2008

    • Author(s)
      Mashima H, Suzuki J, Hirayama T, Yoshikumi Y, Ohno H, Ohnishi H, Yasuda H, Fujta T, Omata M.
    • Journal Title

      Infection And Immunity(掲載確定)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Indian hedgehog promotes the migration of rat activated pancreatic stellate cells by increasing membrane type-1 matrix metalloproteinase on the plasma membrane.2008

    • Author(s)
      Shinozaki S, Ohnishi H, Hama K, Yamamoto H, Osawa, H, Sato K, Tamada K, Mashima H, Sugano K.
    • Journal Title

      Journal of Cellular Physiology(掲載確定)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of membrane-type bile acid receptor M-BAR/TGR5 in bile acid-induced activation of epidermal growth factor receptor and mitogen-activated protein kinases in gastric carcinoma cells2007

    • Author(s)
      Yasuda H, Hirata S, Inoue K, Mashima H, Ohnishi H, Yoshida M.
    • Journal Title

      Biochem Biophys Res Commun 354

      Pages: 154-159

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Autocrine loop between TGF-betal and IL-1beta through Smad3-and ERK-dependent pathways in rat pancreatic stellate cells.2006

    • Author(s)
      Aoki H, Ohnishi H., et al.
    • Journal Title

      Am J Physiol Cell Physiol 290

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Angiotensin II promotes the proliferation of activated pancreatic stellate cells by Smad7 induction through a protein kinase C pathway2006

    • Author(s)
      Hama K., Ohnishi H., et al.
    • Journal Title

      Biochem Biophys Res Commun 342

      Pages: 742-750

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Sonic hedgehog stimulates the proliferation of rat gastric mucosal cells through ERK activation by elevating intracellular calcium concentration.2006

    • Author(s)
      Osawa H., Ohnishi H., et al.
    • Journal Title

      Biochem Biophys Res Commun 344

      Pages: 680-7

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Roles of CTPL/Sfxn3 and Sfxn family members in pancreaticislet.2005

    • Author(s)
      Yoshikumi Y, Mashima H, Ueda N, Ohno H, Suzuki J, Tanaka S, Hayashi M, Sekine N, Ohnishi H, Yasuda H, Iiri T, Omata M, Fujita T, Kojima I.
    • Journal Title

      Journal of Cellular Biochemistry 95

      Pages: 1157-1168

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Roles of CTPL/Sfxn3 and Sfxn family members in pancreatic islet2005

    • Author(s)
      Yoshikumi Y, Mashima H, Ueda N, Ohno H, Suzuki J, Tanaka S, Hayashi M, Sekine N, Ohnishi H, Yasuda H, Iiri T, Omata M, Fujita T, Kojima I.
    • Journal Title

      Journal of Cellular Biochemistry 95

      Pages: 1157-1168

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Roles of CTPL/Sfxn3 and Sfxn family members in pancreatic islet.2005

    • Author(s)
      Yukako Yoshikumi
    • Journal Title

      Journal of Cellular Biochemistry 95・6

      Pages: 1157-1168

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Up-regulation of JAM-1 in AR42J cells treated with activin A and betacellulin and the diabetic regenerating islets

    • Author(s)
      Yoshikumi Y, Ohno H, Suzuki J, Isshiki M, Morishita Y, Ohnishi H, Yasuda H, Omata M, Fujita T, Mashima H.
    • Journal Title

      Endocrine Journal (in press)

    • NAID

      10022609359

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Involvement of VAMP7 in Human Gastric Epithelial Cell Vacuolation Induced by Helicobacter pylori-produced VacA

    • Author(s)
      Mashima H, Suzuki J, Hirayama T, Yoshikumi Y, Ohno H, Ohnishi H, Yasuda H, Fujita T, Omata M.
    • Journal Title

      Infection And Immunity (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The Role of VAMP7 on VacA-induced Vacuolation.2007

    • Author(s)
      Mashima H, Suzuki J, Yoshikumi Y, Ohno H, Omata M.
    • Organizer
      Digestive Disease Week
    • Place of Presentation
      Washington
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] The Role of VAMP7 on VacA-induced Vacuolation2007

    • Author(s)
      Mashima H, Suzuki J, Yoshikumi Y, Ohno H, Omata M.
    • Organizer
      Digestive Disease Week
    • Place of Presentation
      Washington
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 膵の分化におけるJAMの新たな役割2005

    • Author(s)
      吉汲祐加子、真嶋浩聡, 他
    • Organizer
      日本消化器病関連学会週間
    • Place of Presentation
      福岡
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] New Role of JAM-1related to the differentiation of pancreas2005

    • Author(s)
      Yoshikumi Y, Mashima H, Suzuki J, Ohno H, Omata M.
    • Organizer
      Digestive Disease Week Japan
    • Place of Presentation
      Fukuoka
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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