| Project/Area Number |
17590637
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Osaka University |
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Principal Investigator |
TSUTSUI Shusaku Osaka University, Graduate School of Medicine, Assistant, 医学系研究科, 助手 (10359846)
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| Co-Investigator(Kenkyū-buntansha) |
KIYOHARA Tatsuya Osaka University, Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (50322178)
WATABE Kenji Osaka University, Graduate School of Medicine, Assistant, 医学系研究科, 助手 (50379244)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | CD9 / Gastrointestinal Cancer / Apoptosis |
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| Research Abstract |
CD9, a member of the tetraspanin family, has been shown to be involved in a range of cellular activities, including migration, proliferation and adhesion. We found that anti-CD9 monoclonal antibody ALB6 inhibited cell proliferation, reduced cell viability and induced morphological changes specific to apoptosis. ALB6 induced tyrosine phosphorylation of the p46 Shc isoform specifically and that the overexpression of its dominant-negative form completely suppressed the ALB6-induced activation of JNK/SAPK, p38 MAPK and caspase-3, resulting in the inhibition of apoptotic cell death. In this study, we demonstrated that anti-CD-9 monoclonal antibody ALB6 inhibited the tumor growth implanted in the nude mice in vivo.
Method
Gastric cancer cell line(MKN-28)was inplanted in the back of nude mice.Anti-CD9 monoclonal antibody was administrated as the protocol.
Group A control
Group B ALB6 (anti-CD-9 anti body) 100μg/body iv 3 days interval×2 times
Group C ALB6 100 μg/body iv 3 days interval×3 times
Group D ALB6 50μg /body s.c. every day× 12
ALB6 reduced the tumor growth significantly in gtoup B, C, D mice compared to group A mices.
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