| Project/Area Number |
17590645
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Okayama University |
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Principal Investigator |
KOIDE Norio Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Science, Professor (20142333)
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| Co-Investigator(Kenkyū-buntansha) |
SHIRAHA Hidenori Okayama University, University Hospital, Assistant Professor (40379748)
真治 紀之 岡山大学, 医学部・歯学部附属病院, 講師 (70314680)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | 3D culture / liver regeneration / extra cellular matrix |
|---|
| Research Abstract |
To make in vitro model of liver regeneration, we tried to introduce EGF and EGFR cDNAs into rat primary hepatocyte. Introduction of EGFR into rat primary hepatocytes increased 2 PDL. We also tried to find genes to enhance spheroid formation of hepatocyte, since hepatocyte spheroid Is known to have better differentiated function of hepatocyte compared to monolayer culture. We introduced RUNX3, a tumor suppressor gene, into hepatocellular carcinoma cell lines; Hep3B and Huh7. Introduction of RUNX3 cDNA caused conversion of cell shape in these cells. RUNX3 expression increased E-cadherin expression, while it decreased N-cadherin expression. RUNX3 cDNA introduction enhance spheroid formation that respect to better differentatid function of hepatocyte. These results suggest that sequential introduction of EGFR cDNA and RUNX3 cDNA may be a useful method for making in vitro model of liver regeneration.
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