• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Identification of novel bioactive gut peptides and clarification of feeding regulation of brain-gut axis

Research Project

Project/Area Number 17590660
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionUniversity of Miyazaki

Principal Investigator

MONDAL M・S  UNIVERSITY OF MIYAZAKI, FACULTY OF MEDICINE, RESEARCHER, 医学部, 研究員 (00398265)

Co-Investigator(Kenkyū-buntansha) YAMAGUCHI Hideki  UNIVERSITY OF MIYAZAKI, FACULTY OF MEDICINE, ASSISTANT PROFESSOR, 医学部, 助手 (10305097)
NAKAZATO Masamitsu  UNIVERSITY OF MIYAZAKI, FACULTY OF MEDICINE, PROFESSOR, 医学部, 教授 (10180267)
DATE Yukari  UNIVERSITY OF MIYAZAKI, FACULTY OF MEDICINE, PROFESSOR, フロンティア科学実験総合センター, 教授 (70381100)
SHIMBARA Takuya  UNIVERSITY OF MIYAZAKI, FACULTY OF MEDICINE, ASSISTANT PROFESSOR, 医学部, 助手 (10398266)
初 春平  宮崎大学, 医学部, 教務補佐員 (90381102)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsgut peptide / peptidomics analysis / orphan receptor / IMACS / tumor cell lines / RIA
Research Abstract

We hypothesize I that we could utilize a secretopeptidome to identify candidates for novel bioactive peptides, provided we focus on structure of peptides as a hallmark of biologically active peptides. We examined the secretopeptidome prepared from different carcinoma cell lines. Human medullary thyroid carcinoma TT cells actively secretes calcitonin and calcitonin gene-related peptide alpha (CGRPα). We used gel filtration chromatography to obtain a peptide-rich fraction below 10,000 Da in molecular weight, which was subsequently separated into 50 fractions by reverse phase-high performance liquid chromatography. Each fraction was analyzed by off-line nano-ESI MS/MS with a Q-Tof II mass spectrometer (Micromass, Milford, MA) and by MALDI-TOF MS/MS with a Proteomics 4700 mass spectrometer (Applied Biosystems, Foster City, CA). Each MS/MS spectrum was used to probe the NCBI and Swiss-Prot databases with Mascot MS/MS ion search software (Matrix Science, Boston, MA) and also interpreted by SeqMS. Tandem mass spectrometric analysis of column effluent fractions resulted in the identification of 233 peptide sequences, all of which were assigned to known precursors for bioactive peptides or secretory proteins. Of all the peptides identified, we identified novel bioactive peptide using IMACS calcium screening system. The peptide with monoisotopic masses of 2677.4 were derived from a known polypeptide. RT-PCR showed that mRNA of novel peptide was adundantly expressed in gastrointestinal tracts. We established RIA to detect novel bioactive peptide. Among the peripheral tissues, novel bioactive peptide was detected in gastrin-producing cells in the stomach, and in ganglion cells of both intestinal Aueroach and Meissner plexuses, which contain large numbers of additional bioactive peptides that control intestinal motor and secretory functions.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (26 results)

All 2007 2006 2005 2004 Other

All Journal Article (26 results)

  • [Journal Article] Ghrelin stimulates growth hormone secretion and food intake in aged rats.2007

    • Author(s)
      Toshinai K, Mondal MS, Shimbara T, Yamaguchi H, Date Y, Kangawa K, Nakazato M
    • Journal Title

      Mech Ageing Dev 128

      Pages: 182-186

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Ghrelin stimulates growth hormone secretion and food intake in aged rats.2007

    • Author(s)
      Toshinai K, Mondal MS, Shimbara T, Yamaguchi H, Date Y, Kangawa K, Nakazato M
    • Journal Title

      Mech Ageing Dev, 128

      Pages: 182-186

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Central effects of CRSP-1 on energy homeostasis in rats.2006

    • Author(s)
      Sawada H, Yamaguchi H, Shimbara T, Toshinai K, Mondal MS, Date Y, Murakami N, Katafuchi T, Minamino N, Nunoi H, Nakazato M
    • Journal Title

      Endocrinology 147

      Pages: 2043-2050

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Neuropeptide W is present in antral G cells of rat, mouse, and human stomach.2006

    • Author(s)
      Mondal MS, Kawagoe T, Yamaguchi H, Date Y, Kawagoe T, Tsuruta T, Kageyama H, Kawamura Y, Shioda S, Shimomura Y, Mori M, Nakazato M
    • Journal Title

      J Endocrinol 188

      Pages: 49-57

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Identification of hBD-3 in respiratory tract and serum and its increase in pneumonia.2006

    • Author(s)
      Ishimoto H, Mukae H, Date Y, Shimbara T, Mondal MS, Ashitani J, Hiratsuka T, Kubo S, Kohno S, Nakazato M
    • Journal Title

      Eur Respir J 27

      Pages: 253-260

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Des-acyl ghrelin induces food intake by a mechanism independent of the growth hormone secretagogue receptor.2006

    • Author(s)
      Toshinai K, Yamaguchi H, Sun Y, Smith RG, Yamanaka A, Sakurai T, Date Y, Mondal MS, Shimbara T, Kawagoe T, Murakami N, Miyazato M, Kangawa K, Nakazato M
    • Journal Title

      Endocrinology 147

      Pages: 2306-2314

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Neuropeptide W is present in antral G cells of rat, mouse, and human stomach.2006

    • Author(s)
      Mondal MS, Kawagoe T, Yamaguchi H, Date Y, Kawagoe T, Tsuruta T, Kageyama H, Kawamura Y, Shioda S, Shimomura Y, Mori M, Nakazato M.
    • Journal Title

      J Endocrinol 188

      Pages: 49-57

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Identification of hBD-3 in respiratory tract and serum and its increase in pneumonia.2006

    • Author(s)
      Ishimoto H, Mukae H, Date Y, Shimbara T, Mondal MS, Ashitani J, Hiratsuka T, Kubo S, Kohno S, Nakazato M.
    • Journal Title

      Eur Respir J 27

      Pages: 253-260

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Des-acyl ghrelin induces food intake by a mechanism independent of the growth hormone secretagogue receptor.2006

    • Author(s)
      Toshinai K, Yamaguchi H, Sun Y, Smith RG, Yamanaka A, Sakurai T, Date Y, Mondal MS, Shimbara T, Kawagoe T, Murakami N, Miyazato M, Kangawa K, Nakazato M.
    • Journal Title

      Endocrinology 147

      Pages: 2306-2314

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Central effects of CRSP-1 on energy homeostasis in rats.2006

    • Author(s)
      Sawada H, Yamaguchi H, Shimbara T, Toshinai K, Mondal MS, Date Y, Murakami N, Katafuchi T, Minamino N, Nunoi H, Nakazato M.
    • Journal Title

      Endocrinology 147

      Pages: 2043-2050

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Central effects of CRSP-1 on energy homeostasis in rats.2006

    • Author(s)
      Sawada H, Yamaguchi H, Shimbara T, Toshinai K, Mondal MS, Date Y, Murakami N, Katafuchi T, Minamino N, Nunoi H, Nakazato M.
    • Journal Title

      Endocrinology, 147

      Pages: 2043-2050

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Neuropeptide W is present in antral G cells of rat, mouse, and human stomach.2006

    • Author(s)
      Mondal MS, Kawagoe T, Yamaguchi H, Date Y, Kawagoe T, Tsuruta T, Kageyama H, Kawamura Y, Shioda S, Shimomura Y, Mori M, Nakazato M.
    • Journal Title

      J Endocrinol, 188

      Pages: 49-57

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Identification of hBD-3 in respiratory tract and serum and its increase in pneumon a.2006

    • Author(s)
      Ishimoto H, Mukae H, Date Y, Shimbara T, Mondal MS, Ashitani J, Hiratsuka T, Kubo S, Kohno S, Nakazato M.
    • Journal Title

      Eur Respir J, 27

      Pages: 253-260

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Des-acyl ghrelin induces food intake by a mechanism independent of the growth hormone secretagogue receptor.2006

    • Author(s)
      Toshinai K, Yamaguchi H, Sun Y, Smith RG, Yamanaka A, Sakurai T, Date Y, Mondal MS, Shimbara T, Kawagoe T, Murakami N, Miyazato M, Kangawa K, Nakazato M.
    • Journal Title

      Endocrinology, 147

      Pages: 2306-2314

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Identification of ghrelin neurons and its receptor, GHS-R, in rat brain2005

    • Author(s)
      Mondal MS, Date Y, Yamaguchi H, Toshinai K, Kangawa K, Nakazato M
    • Journal Title

      Regul Pept 126

      Pages: 55-59

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Impact of Helicobacter pylori infection on gastric and plasma ghrelin dynamics in humans.2005

    • Author(s)
      Isomoto H, Ueno H, Saenko VA, Mondal MS, Nishi Y, Kawano N, Ohnita K, Mizuta Y, Ohtsuru A, Yamashita S, Nakazato M, Kohno S
    • Journal Title

      Am J Gastroenterol 100

      Pages: 1711-1720

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Identification of ghrelin neurons and its receptor, GHS-R, in rat brain.2005

    • Author(s)
      Mondal MS, Date Y, Yamaguchi H, Toshinai K, Kangawa K, Nakazato M
    • Journal Title

      Regul Pept 126

      Pages: 55-59

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Impact of Helicobacter pylori infection on gastric and plasma ghrelin dynamics in humans.2005

    • Author(s)
      Isomoto H, Ueno H, Saenko VA, Mondal MS, Nishi Y, Kawano N, Ohnita K, Mizuta Y, Ohtsuru A, Yamashita S, Nakazato M, Kohno S.
    • Journal Title

      Am J Gastroenterol 100

      Pages: 1711-1720

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Impact of Helicobacter pylori infection on gastric and plasma ghrelin dynamics in humans.2005

    • Author(s)
      Isomoto H, Ueno H, Saenko VA, Mondal MS, Nishi Y, Kawano N, Ohoita K, Mizuta Y, Ohtsuru A, Yamashita S, Nakazato M, Kohno S
    • Journal Title

      Am J Gastroenterol 100

      Pages: 1711-1720

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Identification of hBD-3 in respiratory tract and serum and its increase in pneumonia.2005

    • Author(s)
      Ishimoto H, Mukae H, Date Y, Shimbara T, Mondal MS.Ashitani J, Hiratsuka T, Kubo S, Kohno S, Nakazato M
    • Journal Title

      Eur Respir J 27

      Pages: 253-260

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Central administration of ghrelin preferentially enhances fat ingestion.2004

    • Author(s)
      Shimbara T, Mondal MS, Kawagoe T, Toshinai K, Koda S, Yamaguchi H, Date Y, Nakazato M.
    • Journal Title

      Neurosci Lett 369

      Pages: 75-79

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Ontogeny of a New Enteric Peptide, Neuropeptide W (NPW), in the Developing Rat Stomach.

    • Author(s)
      Mondal MS, Yamaguchi H, Date Y, Tsuruta T, Shimbara T, Toshinai K, Shimomura Y, Mori M, Nakazato M
    • Journal Title

      Regul Pept. (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Ontogeny of a New Enteric Peptide, Neuropeptide W (NPW), in the Developing Rat Stomach.

    • Author(s)
      Mondal MS, Yamaguchi H, Date Y, Tsuruta T, Shimbara T, Toshinai K, Shimomura Y, Mori M, Nakazato M.
    • Journal Title

      Regul Pept. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Ontogeny of a New Enteric Peptide, Neuropeptide W(NPW), in the Developing Rat Stomach.

    • Author(s)
      Mondal MS, Yamaguchi H, Date Y, Tsuruta T, Shimbara T, Toshinai K, Shimomura Y, Mori M, Nakazato M.
    • Journal Title

      Regul Pept. (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Des-acyl ghrelin induces food intake by a mechanism independent of the growth hormone secretagogue receptor

    • Author(s)
      Toshinai K, Yamaguchi H, Sun Y, Smith RG, Yamanaka A, Sakurai T, Date Y, Mondal MS, Shimbara T, Kawagoe T, Murakami N, Miyazato M, Kangawa K, Nakazato M
    • Journal Title

      Endocrinology, (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Central effects of CRSP-1 on energy homeostasis in rats.

    • Author(s)
      Sawada H, Yamaguchi H, Shimbara T, Toshinai K, Mondal MS, Date Y, Murakami N, Katafuchi T Minamino N, Nunoi H, Nakazato M
    • Journal Title

      Endocrinology, (in press)

    • Related Report
      2005 Annual Research Report

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi