| Project/Area Number |
17590689
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Gastroenterology
|
|---|
| Research Institution | Hyogo College of Medicine |
|---|
Principal Investigator |
FUKUDA Yoshihiro Hyogo College of Medicine, Faculty of Medicine, Professor, 医学部, 教授 (60148640)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TAMURA Kazuo Hyogo College of Medicine, Faculty of Medicine, Associate Professor, 医学部, 助教授 (20278823)
|
|---|
| Project Period (FY) |
2005 – 2006
|
| Project Status |
Completed (Fiscal Year 2006)
|
| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥2,200,000 (Direct Cost: ¥2,200,000)
|
|---|
| Keywords | Crohn's disease / Inflammatory Bowel Disease / cDNA microarray / Gene Expression |
|---|
| Research Abstract |
PURPOSE : The causes of Crohn's disease (CD) are unknown. However oral administration of probiotics and synbiotics may be beneficial, since intestinal contents-and bacteria-related abnormality of immunity is proposed to be involved. In CD, there was obsereved overproduction of inflammatory cytikine such as TNF-alpha, IL-1 beta and IL-6, and biological preparations and immunosuppressive agents have been administered for restraint or normalization of such overproduction. Here we examined the possibility of prevention and therapy of inflammatory bowel disease by oral administration of koji (Aspergillus orizae NK-Koji) fermentation components containing probiotics and koji.
SUBJECTS AND METHODS : We collected the peripheral leucocytes from ten CD patients and analyzed the gene expression using cDNA microarray method. Approximately ten thousands genes were used for the analysis. Crohn' s Disease Activity Index (CDAI) and CRP, erythrocyte sedimentation rate and others were used for disease status and inflammatory index, respectively.
RESULTS : There were about 40 up-regulated genes and about 50 down-regulated genes prior to the treatment, compared to the analysis using genes from healthy controls. The expression of some genes was changed during the treatment, and there were about 50 genes whose gene expression might be changed in relation to CD activity. Those genes included THF-alpha, IL-1 beta, IL-4 and IL-12, and the results were similar to those reported previously.
CONCLUSIONS : Oral administration of koji fermentation components containing probiotics and koji resulted in the improvement of CD disease status. One of the mechanisms will be suppression of Th-1 cytokines. It is expected to develop novel diagnosis tools and therapies for CD by the analysis of gene expression of inflammatory cytokines and other genes detected by cDNA microarray method.
|
