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Analysis of cardiac specific transcription factor GATA-4 complex during differentiation of mouse embryonic stem cells into cardiac myocytes

Research Project

Project/Area Number 17590770
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionResearch Institute for Production Development

Principal Investigator

MORIMOTO Tatsuya  Research Institute for Production Development, Laboratory of Cardiovascular Molecular Cellular Biology, Chief, 心血管分子細胞生物学研究室, 主任研究員 (50390779)

Co-Investigator(Kenkyū-buntansha) KITA Toru  Kyoto University, Department of Cardiovascular Medicine, Professor, 大学院医学研究科循環器内科, 教授 (60161460)
ONO Koh  Organization Kyoto Medical Center, Division of Translational Research National Hospital, Laboratory Chief, 臨床研究センター展開医療研究部・生命情報科学研究室, 室長 (00359275)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsembryonic stem (ES) cells / Proteomics / Cardiac myocytes differentiation / Transcription factor / GATA-4
Research Abstract

Differentiation of embryonic stem (ES) cells into cardiac myocytes requires activation of a cardiac-specific gene program. Histone acetyltransferases (HATs) and histone deacetylases (HDACs) govern gene expression patterns by being recruited to target genes through association with specific transcription factors. One of HATs, p300 serves as a coactivator of cardiac-specific transcription factors such as a zinc finger protein GATA4. Treatment of ES cells with trichostatin A (TSA), a specific HDAC inhibitor, induces acetylation of GATA4 as well as histones and facilitates their differentiation into cardiac myocytes. Here, we show that cyclin-dependent kinases-9 (CDK9), a component of positive transcription elongation factor b which increases the activitiy of RNA Pol II by hyperphosphorylation, is a novel component of p300/GATA4 complex and is required for myocardial cell differentiation. CDK9 interacted not only with GATA4 but also with p300. A dominant-negative form of CDK9 (DN-CDK9) and … More CDK9 kinase inhibitor, 5,6-dichloro-1-h-ribofuranosyl-benzimidazole (DRB) inhibited p300-induced activation of GATA4-dependent transcription as well as acetylation of GATA4. We examined the effects of DRB and DN-CDK9 on myocardial differentiation by flow cytometry in a mouse ES cell line, in which GFP is expressed under the control of the cardiac-specific Nkx-2.5 promoter. This line of ES cells was cultured in a monolayer on a flat gelatin-coated plate without embryoid body formation. TSA induced the expression of GFP by 8-to 9-fold in these cells. Furthermore, TSA increased the amount of the GATA4/CDK9 complex in mouse ES cells. Administration of DRB repressed TSA-induced expression of GFP controlled by the Nkx-2.5 promoter. Furthermore, we introduced DN-CDK9 into Nkx-2.5/GFP ES cells by lenti-virus-mediated gene transfer. Introduction of the DN-CDK9 gene decreased TSA-induced GFP expression by 50%, while introduction of the null vector had no effect. These findings demonstrate that CDK9, as a novel component of the p300/GATA4 complex, is involved in the differentiation of mouse ES cells into cardiac myocytes. Less

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (7 results)

All 2007 2006 2005

All Journal Article (6 results) Book (1 results)

  • [Journal Article] Trichostatin A induces myocardial differentiation of monkey ES cells2007

    • Author(s)
      Hosseinkhani M, Hasegawa K, et al.
    • Journal Title

      Biochem Biophys Res Commun 356(2)

      Pages: 386-391

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Trichostatin A induces myocardial differentiation of monkey ES cells2007

    • Author(s)
      Hosseinkhani M, Hasegawa K, Ono K, Kawamura T, Takaya T, Morimoto T, Wada H, Shimatsu A, Prat SG, Suemori H, Nakatsuji N, Kita T.
    • Journal Title

      Biochem Biophys Res Commun. 356(2)

      Pages: 386-91

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Histone acetyltransferase activity of p300 is required for the promotion of left ventricular remodeling following myocardial infarction in adult mice in vivo2006

    • Author(s)
      Miyamoto S, Kawamura T, Morimoto T et al.
    • Journal Title

      Circulation 113(5)

      Pages: 679-690

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Histone acetyltransferase activity of p300 is required for the promotion of left ventricular remodeling following myocardial infarction in adult mice in vivo2006

    • Author(s)
      Miyamoto S, Kawamura T, Morimoto T, Ono K, Kawase Y, Kita T, Hasegawa K
    • Journal Title

      Circulation. 113(5)

      Pages: 679-90

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Acetylation of GATA-4 is involved in the differentiation of embryonic stem cells into cardiac myocytes.2005

    • Author(s)
      Kawamura T, Ono K, Morimoto T et al.
    • Journal Title

      Journal of Biological Chemistry 280(20)

      Pages: 19682-19688

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Acetylation of GATA-4 is involved in the differentiation of embryonic stem cells into cardiac myocytes.2005

    • Author(s)
      Kawamura T, Ono K, Morimoto T, Wada H, Hirai M, Hidaka K, Morisaki T, Heike T, Nakahata T, Kita T, Hasegawa K.
    • Journal Title

      J Biol Chem. 280(20)

      Pages: 19682-8

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 転写因子のアセチル化と心臓リモデリング 細胞工学Vol. 26 No 42007

    • Author(s)
      森本 達也, 長谷川 浩二
    • Total Pages
      5
    • Publisher
      秀潤社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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