| Project/Area Number |
17590791
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Tottori University |
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Principal Investigator |
BURIOKA Naoto Tottori University, Faculty of Medicine, Associate Professor (50252854)
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| Co-Investigator(Kenkyū-buntansha) |
CHIKUMI Hiroki Tottori University, Faculty of Medicine, Assistant Professor (90283994)
SHIMIZU Eiji Tottori University, Faculty of Medicine, Professor (50187449)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2006: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2005: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | Physiology / Internal medicine / gene / circadian rhythm / clock gene / sleep apnea syndrome |
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| Research Abstract |
Clock genes regulate mammalian circadian rhythms, and dysfunction of clock genes can contribute to various disorders. To investigate whether obstructive sleep apnea syndrome (OSAS) influences clock gene function, we examined Period1 (Per1) mRNA expression in vitro and in vivo. In 8 healthy subjects and 8 OSAS patients, we measured plasma noradrenaline, serum IL-6, and high-sensitivity CRP (hs-CRP), and Per1 mRNA expression in peripheral whole blood. Expression of Per1 mRNA in cultured cells was examined under IL-6 or noradrenaline stimulation in vitro. After noradrenaline was administered to mice in vivo, Per1 mRNA expression in the brain was examined. The concentrations of serum IL-6, hs-CRP and plasma noradrenaline were elevated in OSAS patients, but improved by CPAP therapy. Per1 mRNA expression in the peripheral blood at 2:00 h significantly decreased by CPAP in OSAS patients. The stimulation with IL-6 did not directly induce Per1 mRNA in vitro. The administration of noradrenaline induced Per1 mRNA in cerebral cortex of mice in vivo. We first revealed that OSAS caused clock gene dysfunction, and CPAP helped to improve it. Sympathetic activation and elevation of the plasma noradrenaline concentration in OSAS may be one of the factors involved in disorders of Per1 mRNA expression.
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