Investigation and regulation of molecular mechanisms of epithelial mesenchymal transition in pulmonary fibrosis
| Project/Area Number |
17590793
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | KYUSHU UNIVERCITY |
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Principal Investigator |
KUWANO Kazuyoshi Kyushu University, Graduate School of Medical Sciences, Associate Professor, 大学院医学研究院, 助教授 (40205266)
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| Co-Investigator(Kenkyū-buntansha) |
FUJITA Masaki Kyushu University, Hospital, Research associate, 大学病院, 助手 (50325461)
MAEYAMA Takashige Kyushu University, Hospital, Medical Staff, 大学病院, 医員 (40380456)
吉見 通洋 九州大学, 大学病院, 助手 (30372759)
猪島 一朗 九州大学, 大学病院, 医員 (90380419)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | pulmonary fibrosis / lung injury / EMT / apoptosis / bleomycin / TGF-beta / 特発性間質性肺炎 / 特発性肺線維症 |
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| Research Abstract |
Epithelial-mesenchymal transition (EMT) has been considered to be involved in organ fibrogenesis. However, there is few direct evidence of this process in the pathophysiology of pulmonary fibrosis in vivo. Therefore, we tried to verify the involvement of this process in the development of pulmonary fibrosis. Since the co-expressions of epithelial and mesenchymal markers are thought to be a marker of EMT, we performed dual immunohistochemistry to assess the co-expressions of these proteins in lung tissues from bleomycin-induced pulmonary fibrosis in mice, and from patients with idiopathic pulmonary fibrosis and nonspecific interstitial pneumonia. Double positive cells for epithelial markers including E-cadherin, CD44v10,T1α, or aquaporin 5,and a mesenchymal marker α-smooth muscle actin were rarely found in bleomycin-induced pulmonary fibrosis in mice. Double positive cells for E-cadherin, ICAM-1,LEA, CD44v9,or SP-A and a-smooth muscle actin were not found in lung tissues from idiopathic pulmonary fibrosis and nonspecific interstitial pneumonia. In contrast to few incidence of EMT, we occasionally found intermediate cells which have characteristics of type I and type II or bronchiolar epithelial cells. These results suggest that EMT may be present in pulmonary fibrosis, but have minor role in the pathophysiology of pulmonary fibrosis.
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Report
(3 results)
Research Products
(19 results)
