| Project/Area Number |
17590800
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | OSAKA CITY UNIVERSITY |
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Principal Investigator |
KANAZAWA Hirosh OSAKA CITY UNIVERSITY, GRADUATE SCHOOL OF MEDICINE, LECTURER, 大学院医学研究科, 講師 (90332957)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | VEGF / microvascular permeability / angiopoietin-1 / angiopoietin-2 / bronchial asthma / 誘発喀痰 / 微小循環 / 血管内皮細胞 / 喘息 / 肺生検 / 血管透過性 |
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| Research Abstract |
Vascular endothelial growth factor (VEGF) increases microvascular permeability. Recently, considerable attention has been devoted to the physiological roles of angiopoietin-1 and-2 as regulatory factors of VEGF. This study was designed to examine the roles of angiopoietin-1 and-2in controlling airway microvascular permeability in asthma. Levels of these angiogenic factors and airway vascular permeability index were examined in 30 asthmatics and 12 control subjects. After 2-week run-in period, all asthmatics were randomly assigned to receive fluticasone propionate (400 μg/day) or montelukast (10 mg) for 12 weeks. VEGF, angiopoietin-1 and-2 levels in induced sputum were significantly higher in asthmatics than in control subjects. We found an inverse correlation between angiopoietin-1 level and vascular permeability index in asthmatics, while there was a positive correlation between angiopoietin-2 level and that index. VEGF and angiopoietin-1 levels were significantly decreased after fluticasone therapy, while VEGF and angiopoietin-2 levels were significantly decreased after montelukast therapy. Though VEGF levels after treatment were different between two groups, vascular permeability index in the montelukast group was the same level as that in the fluticasone group. Moreover, improvement in vascular permeability index after fluticasone therapy was inversely correlated with decrease in angiopoietin-1 level, while that after montelukast therapy was positively correlated with decrease in angiopoietin-2 level. Angiopoietin-1 and-2 play complementary and coordinated roles in regulating microvascular permeability stimulated by VEGF in asthma. Combination of corticosteroids with leukotriene antagonists might effectively improve plasma leakage and provide a new strategy in treating bronchial asthma.
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