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Analysis of bone-marrow derived fibroblasts involved in asthmatic airway remodeling and its interventional control of function

Research Project

Project/Area Number 17590802
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionIwate Medical University

Principal Investigator

YAMAUCHI Kohei  Iwate Med Univ, Scl Med, associate professor, 医学部, 助教授 (20200579)

Co-Investigator(Kenkyū-buntansha) MURAI Kazunori  Iwate Med Univ, Scl Med, assistant professor, 医学部, 助手 (70285592)
OHTSU Hiroshi  Tohoku Univ, Dep Technology, professor, 工学部, 教授 (60250742)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2005: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordsasthma / allergic airway inflammation / remodeling / fibroblast / GFP / Bone marrow derived cells / collagen / mouse / 骨髄キメラマウス / G-CSF / コラーゲン沈着 / 線維化 / 気管支喘息 / 気道リモデリング / 粘膜線維化 / 骨髄細胞
Research Abstract

Analysis of the asthma model with bone marrow transferred chimeric mice
Method : We made the murine asthma model with bone marrow transferred chimeric C57BL/6 mice. At two weeks after sensitization with Ovalbumin (OVA), the mice were exposed to OVA every other day during one month. Twenty four hours later from final exposure with OVA, we collected blood and broncho-alveolar lavage fluid (BAL) and obtained the lung tissue from these mice on 7^<th> day, 14^<th> day and 28^<th> day. In addition, we divided these mice into two groups. One group of the mice was administered intraperitoneally with G-CSF and the other group with saline as the control.
Estimation of histological changes was performed. The pulmonary tissue was fixed with formaldehyde and were embedded in paraffin. On the tissue sections, we estimated infiltrated inflammatory cells into airway wall as a score semi-quantitatively with Hematoxylin-Eosin staining. We identified GFP-expressing cells in bronchial tissues under chemiluminescence microscopy and also GFP positive cells with immunohistochemistry using ant-GFP antibody.
Results : On 7^<th> day after OVA exposure, eosinophils as major cells, lymphocytes and monocytes were observed in peribronchial and alveolar tissue in the chimeric mice. On 14^<th> and 28^<th> day, thickening of epithelial basement membrane and deposition of collagen in submucosal layer of bronchi became prominent. Some GFP-expressing cells were spindle-like shape under epithelium and assumed fibroblast like cells. They were also confirmed by immunohistochemistry.
GFP-expressing cells increased more in the lung of the mice with G-CSF treatment than control, and collagen deposition estimated with Elastica-Masson staining decreased in G-CSF treated mice compared to control.
Conclusion : Bone marrow derived cells were recruited to peribronchial tissue with allergic inflammation and might play a regulatory role in collagen deposition in the airway.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (24 results)

All 2006 2005

All Journal Article (24 results)

  • [Journal Article] Recent advances in molecular pharmacology of the histamine systems : OCT as a histamine transporter and histamine metabolism.2006

    • Author(s)
      Ogasawara M et al.
    • Journal Title

      J Pharmacol Sci 101

      Pages: 24-30

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Klinefelter's syndrome associated with systemic lupus erythematosus and autoimmune hepatitis.2006

    • Author(s)
      Sasaki N et al.
    • Journal Title

      Mod Rheumatol 16

      Pages: 305-308

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Airway remodeling in asthma and its influence on clinical pathophysiology.2006

    • Author(s)
      Yamauchi K et al.
    • Journal Title

      Tohoku J Exp Med 209

      Pages: 75-87

    • NAID

      10017492884

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Recent advances in molecular pharmacology of the histamine systems : organic cation transporters as a histamine transporter and histamine metabolism.2006

    • Author(s)
      Ogasawara M, Yamauchi K, Satoh Y, Yamaji R, Inui K, Jonker JW, Schinkel AH, Maeyama K
    • Journal Title

      Pharmacol Sci. 101(1)

      Pages: 24-30

    • NAID

      10018237241

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Klinefelter's syndrome associated with systemic lupus erythematosus and autoimmune hepatitis.2006

    • Author(s)
      Sasaki N, Yamauchi K, Sato R, Masuda T, Sawai T, Inoue H.
    • Journal Title

      Mod Rheumatol. 16(5)

      Pages: 305-8

    • NAID

      10018307616

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Airway remodeling in asthma and its influence on clinical pathophysiology.2006

    • Author(s)
      Yamauchi K.
    • Journal Title

      Tohoku J Exp Med. 209(2)

      Pages: 75-87

    • NAID

      10017492884

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Airway remodeling in asthma and its influence on clinical pathophysiology.2006

    • Author(s)
      Yamauchi, K
    • Journal Title

      Tohoku J Exp Med. 209

      Pages: 75-87

    • NAID

      10017492884

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Recent advances in molecular pharmacology of the histamine systems2006

    • Author(s)
      Ogasawara M
    • Journal Title

      J Pharmacology Sci 101

      Pages: 24-30

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Klinefelter's syndrome associated with systemic lupus erythematosus and autoimmune hepatitis2006

    • Author(s)
      Sasaki N
    • Journal Title

      MOd Rheumatol 16

      Pages: 305-308

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 日本人の若年者(10歳から20歳)の呼吸機能検査の基準値2006

    • Author(s)
      田村弦
    • Journal Title

      日本呼吸器学会雑誌 44

      Pages: 916-922

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 好中球エラスターゼによる気道傷害に対するストレス誘導蛋白の抑制効果2006

    • Author(s)
      伊藤晴方
    • Journal Title

      アレルギー 55

      Pages: 820-826

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Increased levels of CTGF mRNA expression in a murine model of allergic airway inflammation.2005

    • Author(s)
      Piao H-M et al.
    • Journal Title

      Allergology International 127

      Pages: 731-737

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Efficacy and safety of intravenous theophylline administration for treatment of mild acute exacerbation of bronchial asthma.2005

    • Author(s)
      Yamauchi K et al.
    • Journal Title

      Respirology 10

      Pages: 491-496

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Anti-inflammatory actions of pranlukast, and its efficacy on asthma QOL.2005

    • Author(s)
      Yamauchi K et al.
    • Journal Title

      Proceeding of the 6th Asia Pacific Congress of Allergology and Clinical Immunology : 2004,October 4-7.Tokyo

      Pages: 113-116

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Increased levels of CTGF mRNA expression in a murine model of allergic airway inflammation.2005

    • Author(s)
      Piao H-M, Yamauchi K, Pan L-H, Nakadate T, Ito H, Mouri T, Kobayashi H, Sawai T, Nakanishi T, Takigawa M, Inoue H
    • Journal Title

      Allergology International 54

      Pages: 105-117

    • NAID

      10014468908

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Low plasma orexin-a levels were improved by continuous positive airway pressure treatment in patients with severe obstructive sleep apnea-hypopnea syndrome.2005

    • Author(s)
      Sakurai S, Nishijima T, Takahashi S, Yamauchi K, Arihara Z, Takahashi K
    • Journal Title

      Chest 127

      Pages: 731-737

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Efficacy and safety of intravenous theophylline administration for treatment of mild acute exacerbation of bronchial asthma.2005

    • Author(s)
      Yamauchi K, Kobayashi H, Tanifuji Y, Yoshida T, Piao H-M, Inoue H
    • Journal Title

      Respirology 10(4)

      Pages: 491-6

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Anti-inflammatory actions of pranlukast, and its efficacy on asthma QOL.2005

    • Author(s)
      Yamauchi K, Kobayashi H, Inoue H, Tamura G, Tohoku Onon Forum Study Group
    • Journal Title

      Proceeding of the 6th Asia Pacific Congress of Allergology and Clinical tmmunology : 2004, October 4-7.Tokyo : Monduzzi Editore

      Pages: 113-116

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The role of histamine in goblet cell hyperplasia in allergic airway inflammation-A study using the HDC knockout mouse-.2005

    • Author(s)
      Piao H-M, Yamauchi K, Nakamura Y, Nakadate T, Ito H, Mouri T, Kobayashi H, Ohtsu H, Inoue H
    • Journal Title

      Proceeding of the 6th Asia Pacific Congress of Allergology and Clinical Immunology : 2004, October 4-7.Tokyo : Monduzzi Editore

      Pages: 193-195

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Increased levels of CTGF mRNA expression in a murine model2005

    • Author(s)
      Piao-H-M et al.
    • Journal Title

      Allergology International 54

      Pages: 105-117

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Low plasma orexin-levels were improved by continuous positive airway pressure2005

    • Author(s)
      Sakurai S et al.
    • Journal Title

      Chest 127

      Pages: 731-737

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Efficacy and safety of intravenous theophylline administration2005

    • Author(s)
      Yamauchi K et al.
    • Journal Title

      Respirology 10

      Pages: 491-496

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Anti-inflammatory actions of pranlukast and its efficacy on asthma QoL2005

    • Author(s)
      Yamauchi
    • Journal Title

      Proceedings of 6th Asia Pacific Congress

      Pages: 113-116

    • Related Report
      2005 Annual Research Report
  • [Journal Article] The role of histamine in goblet cell hyperplasia in allergic airway inflammation2005

    • Author(s)
      Piao H-M et al.
    • Journal Title

      Proceeding of 6th Asia Pacific Congress

      Pages: 193-195

    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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