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The role of intercellular communication in repair process of glomerular injury

Research Project

Project/Area Number 17590821
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionNiigata University

Principal Investigator

MORIOKA Tetsuo  Niigata University, Institute of Medicine and Dentistry, Associate Professor, 医歯学系, 助教授 (00210146)

Co-Investigator(Kenkyū-buntansha) KUMAGAI Naoki  Niigata University, Medical and Dental Hospital, Medical Staff, 医歯学総合病院, 医員 (30377189)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsGap junction / Glomerular mesangial cell / Glomerular endothelial cell / Connexin 37 / Connexin 40 / Connexin 43 / Glomerular sclerosis / Cell adhesion / メサンギクム細胞
Research Abstract

The purpose of this study was to clarify the role of gap junctional intercellular communication (GJIC) in repair process of glomerular injury. Using cell culture system, we investigated the effect of LPS, TNF-α,IFN-γ to the GJIC and connexin expression of cultured human glomerular endothelial cells. Using lucifer yellow as an indicator, dye transfer assay revealed that LPS and TNF-α increased and IFN-γ decreased the GIJC in glomerular endothelial cells. Using real time RT-PCR, mRNA expression of connexin37,40,43 in cultured human endothelial cells was assessed. Cx43 mRNA expression was increased 24 hours after addition of LPS and TNF-α, but decreased after addition of IFN-γ. Cx37,40 mRNA expression was decreased after addition of LPS and TNF-α, but not changed after addition of IFN-γ. We also examined the change of connexin40,43 expression during the course of experimental mesangial proliferative GN model. Immunofluorescence and immunoelectron micropsopic study showed that in normal control rats, Cx40 expressed on the intra and extra-glomerular mesangial cells. Cx40 was presented on the endothelial cells and smooth muscle cells of extra glomerular vessels of kidney. The distribution of Cx43 was similar to that of Cx40. Three to seven days after induction of glomerulonephritis, expression of Cx40 and 43 was decreased. Fourteen days after induction of glomerulonephritis, the expression was recovered similar to normal state. From these data, we concluded that GJIC may contribute to the repair process of glimerular injury.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (20 results)

All 2007 2006 2005 Other

All Journal Article (20 results)

  • [Journal Article] Alterations of cell adhesion molecules in human glomerular endothelial cells in response to nephritis-associated plasminogen receptor (NaPlr)2007

    • Author(s)
      Khan F, Yamakami K, Mahmood J, Li B, Kikuchi T, Kumagai N, Morioka T, Yoshizawa N, Oite T
    • Journal Title

      Nephron 105

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Alterations of cell adhesion molecules in human glomerular endothelial cells in response to nephritis-associated plasminogen receptor(NaPlr)2007

    • Author(s)
      Khan F, Yamakami K, Mahmood J, Li B, Kikuchi T, Kumagai N, Morioka T, Yoshizawa N, Oite T
    • Journal Title

      Nephron 105

    • Related Report
      2006 Annual Research Report
  • [Journal Article] SYNERGISTIC EFFECTS of PDGF-BB and cAMP-ELEVATING AGENTS ON THE EXPRESSION OF CONNEXIN43 IN MESANGIAL CELLS2006

    • Author(s)
      Yao J, Kitamura M, Zhu Y, Meng Y, Kasai A, Hiramatsu N, Morioka T, Takeda M, Oite T
    • Journal Title

      American Journal of Physiology Renal Physiology 290

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Bone marrow cell infusion ameliorates progressive glomerulosclerosis in an experimental rat model2006

    • Author(s)
      Li B, Morioka T, Uchiyama M, Oite T
    • Journal Title

      Kidney International 69

      Pages: 323-330

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Turbulence of glomerular hemodynamics involved in progressive glomerulosclerosis2006

    • Author(s)
      Kawamura K, Okada S, Li B, Suwa M, Yao J, Morioka T, Gejyo F, Oite T
    • Journal Title

      Kidney International 69

      Pages: 1792-1798

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Local delivery of angiotensin receptor blocker into the kidney amelioreates progression of experimental glomerulonephritis2006

    • Author(s)
      Mahmood J, Khan F, Okada S, Kumagai T, Morioka T, Oite T
    • Journal Title

      Kidney International 70

      Pages: 1591-1598

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Synergistic effects of PDGF-BB and cAMP-elevating agentson the expression of connexin43 in mesangial cells2006

    • Author(s)
      Yao J, Kitamura M, Zhu Y, Meng Y, Kasai A, Hiramatsu N, Morioka T, Takeda M, Oite T
    • Journal Title

      American journal of Physiology 290

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Bone marrow cell inffusion ameliorates progressive glomerulosclerosis in an experimental rat model2006

    • Author(s)
      Li B, Morioka T, Uchiyama M, Oite T
    • Journal Title

      Kidney International 69

      Pages: 323-330

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Local delivery of angiotensin receptor blocker into the kidney amelioreates progression ofexperimental elomerulonephritis2006

    • Author(s)
      Mahmood J, Khan F, Okada S, Kumagai N, Morioka T, Oite T
    • Journal Title

      Kidney International 70

      Pages: 1591-1598

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Alterations of cell adhesion molecules in human glomerular endothelial cells in response to nephritis-associated plasminogen receptor (NaPlr) Nephron2006

    • Author(s)
      Khan F, Yamakami K, Mahmood J, Li B, Kikuchi T, Kumagai N, Morioka T, Yoshizawa N, Oite T
    • Journal Title

      Nephron Experimental Nephrology 105

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Local delivery of angiotensin receptor blocker into the kidney amelioreates progression of experimental glomerulonephritis2006

    • Author(s)
      Mahmood J, Khan F, Okada S, Kumagai N, Morioka T, Oite T
    • Journal Title

      Kidney International 70

      Pages: 1591-98

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Establishment of immortalized human glomerular endothelial cell lines and their application2005

    • Author(s)
      Harada T, Batsford S, Morioka T, Yao J, Arakawa M, Gejyo F, Oite T
    • Journal Title

      Nephron Experimental Nephrology 99(2)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Nitric Oxide-Mediated Regulation of Connexin43 Expression and Gap Junctional Intercellular Communication in Mesangial Cells2005

    • Author(s)
      Yao J, Hiramatsu N, Zhu Y, Morioka T, Takeda M, Oite T, Kitamura M
    • Journal Title

      Journal of the American Society of Nephrology 16(1)

      Pages: 58-67

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Bone marrow cells contribute to regeneration of damaged glomerular endothelial cells2005

    • Author(s)
      Ikarashi K, Li B, Suwa M, Kawamura K, Morioka T, Yao J, Khan F, Uchiyama M, Oite T
    • Journal Title

      Kidney International 67

      Pages: 1925-1933

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Gap junction in glomerular mesangial cells2005

    • Author(s)
      Yao J, Oite T, Morioka T, Kitamura M
    • Journal Title

      Yamanashi Medical Journal 20

      Pages: 1-12

    • NAID

      110004860870

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Establishment of immortalized human glomerular endothelial cell lines and their application2005

    • Author(s)
      Harada T, Batsford S, Morioka T, Yao J, Arakawa M, Gejyo F, Oite T
    • Journal Title

      Nephron Experimental Nephrology 99

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Nitric Oxide-Mediated Regulation of Connexin43 Expression and Gap Junctional Intercellular Communication in Mesangial Cells2005

    • Author(s)
      Nephron Experimental Nephrology 99:e38-e45, 2005 Yao J, Hiramatsu N, Zhu Y, Morioka T, Takeda M, Oite T, Kitamura M
    • Journal Title

      Journal of the American Society of Nephrology 16

      Pages: 58-67

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Bone marrow cells contribute to regeneration of damaged glomerularendothelial cells2005

    • Author(s)
      Ikarashi K, Li B, Suwa M, Kawamura K, Morioka T, Yao J, Khan F, Uchiyama M, Oite T
    • Journal Title

      Kidney International 67

      Pages: 1925-33

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Gap junction in glomerular mesangial cells

    • Author(s)
      Yao J, Oite T, Morioka T, Kitamura M
    • Journal Title

      Yamanashi Medical Journal

    • NAID

      110004860870

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] SYNERGISTIC EFFECTS of PDGF-BB and cAMP-ELEVATING AGENTS ON THE EXPRESSION OF CONNEXIN43 IN MESANGIAL CELLS

    • Author(s)
      Yao J, Kitamura M, Zhu Y, Meng Y, Kasai A, Hiramatsu N, Morioka T, Takeda M, Oite T
    • Journal Title

      American Journal of Physiology Renal Physiology 2005 Nov1 ; [Epub ahead of print]

    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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