• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis on aggregation formation by novel Lewy body component Siah-1 complementary to Parkin

Research Project

Project/Area Number 17590880
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurology
Research InstitutionHiroshima University

Principal Investigator

TAKAHASHI Tetsuya (2006)  Hiroshima University, Graduate School of Biomedical Sciences, Assistant professor (00435942)

山下 拓史 (2005)  広島大学, 大学院・医歯薬学総合研究科, 助手 (20311813)

Co-Investigator(Kenkyū-buntansha) HATTORI Nobutaka  Juntendo, University, School of Medicine, Professor (80218510)
MATSUMOTO Masayasu  Hiroshima University, Graduate School of Biomedical Sciences, Professor (20192346)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2005: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordsneurological disorder / neuroscience / gene / protein
Research Abstract

Synphilin-1 is a protein reported as a component of Lewy bodies, which characterize Parkinson's disease pathologically. In this study, we established synphilin-1 transgenic mice to analyze as model animal of Parkinson's disease.
We generated transgenic mice expressing human synphilin-1 under the prion protein promoter.
Synphilin-1 was widely expressed in neurons in the brain including the substantia nigra, where massive loss of dopamine neurons was not observed.
In the transgenic mouse brain, synphilin-1 protein was polyubiquitinated, and partially insoluble.
Although modified-SHIRPA revealed no significant difference in behavior and morphology, the reduced rotarod performance and step length were observed in transgenic mice as compared with non-transgenic littermates. Synphilin-1 might be involved in motor function, and its accumulation in the central nervous system can cause motor impairments.
We are now analyzing the mechanisms, by which transgenic mice showed motor impairment through the measurement of dopamine-metabolies in brain by HPLC.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (5 results)

All 2008 2006

All Journal Article (5 results)

  • [Journal Article] Synphilin-1 transgenic mice exhibit mild motor impairments.2008

    • Author(s)
      Jin HG
    • Journal Title

      Neurosci Lett. 445(1)

      Pages: 12-17

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Synphilin-1 transgenic mice exhibit mild motor impairments.2008

    • Author(s)
      Jin HG, et al.
    • Journal Title

      Neurosci Lett. 445(1)

      Pages: 12-17

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Interactions of Synphilin-1 with phospholipids and lipid membranes.2006

    • Author(s)
      Takahashi T
    • Journal Title

      FEBS Lett. 580(18)

      Pages: 4479-4484

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Interactions of Synphilin-1 with phospholipids and lipid membranes.2006

    • Author(s)
      Takahashi T, et al.
    • Journal Title

      FEBS Lett. 580(18)

      Pages: 4479-4484

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Embryonic stem cell-derived neuron models of Parkinson's disease exhibit delayed neuronal death2006

    • Author(s)
      Yamashita H, et al.
    • Journal Title

      J Neurochem in press

    • Related Report
      2005 Annual Research Report

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi