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An impact of HSP genes as new therapeutic target moleculesf or diabetic neplunpathy

Research Project

Project/Area Number 17590926
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Metabolomics
Research InstitutionShiga University of Medical Science

Principal Investigator

ARAKI Shin-ichi  Shiga University of Medical Science, Faculty of Medicine, Assistant Professor (80378455)

Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,710,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsDiabetes / Diabetic Npehropathy / gene
Research Abstract

To explore new therapeutic target molecules which involve in the development of diabetic nephropathy, we created a time course data of gene expression profiles in the diabetic kidney by a DNA microarray method. Gene expression of the genes which belonged to Heat Shock Protein (HSP), stress responsiveness chaperonin, was inhibited in the kidney of the type 2 diabetic model mice, db/db mice, in comparison to the control mice, db/m nice, over the time-course. The protein levels of HSP70 were increased in the kidney from db/db mice only at the early course of diabetes and were then decreased. In cultured mesengial cells, high glucose medium induced the HSP70 expression, whereas oxidative stress inhibited the mRNA levels and protein levels of HSP70. In the mesangial cells which were constitutively over-expressed HSP70, oxidative stress-induced apoptosis was prevented. Erythropoietin which has a reno-protective effect was induced HSP70 expression in the renal cells and the kidney. These results suggest that, in the kidney of type 2 diabetic model mice, HSP70 expression first increases in response to hyperglycemia and then decreases due to oxidative stress enhanced by chronic diabetic condition. Thus, the failure of the anti-oxidative stress defense mechanism of HSP70 may involve in the development of diabetic nephropathy and an induction of HSP70 might be a tool to prevent diabetic nephropathy

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (4 results)

All 2008 2007

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (2 results)

  • [Journal Article] Asialoerythropoietin prevents contrast-induced nephropathy.2008

    • Author(s)
      Yokomaku Y, et. al.
    • Journal Title

      J Am Soc Nephrol 19

      Pages: 321-328

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Asialoerythropoietin prevents contrast-induced nephropathy2008

    • Author(s)
      Yokomaku, Y., Sugimoto, T., Kume, S., Araki, S., Isshiki, K., Chin-Kanasaki, M., Sakaguchi, M., Nitta, N., Haneda, M., Koya, D., Uzu, T., Kashiwagi, A
    • Journal Title

      J Am Soc Nephrol 19(2)

      Pages: 321-328

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] アシアロエリスロポエチンは、造影剤腎症の発症を抑制する2007

    • Author(s)
      横幕由喜代, 他
    • Organizer
      第50回日本腎臓学会学術総会
    • Place of Presentation
      浜松
    • Year and Date
      2007-05-27
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Asialoerythropoietin prevents contrast-induced nephropathy2007

    • Author(s)
      Yokomaku, Y., Sugimoto, T., Kume, S., Sakaguchi, M., Chin-Kanasaki, M., Isshiki, K., Araki, S., Higuchi, M., Haneda, M., Koya, D., Uzu, T
    • Organizer
      The 50st Annual Meeting Japanese Society of Nephrology
    • Place of Presentation
      Hamamatu
    • Year and Date
      2007-05-27
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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