Elucidation of susceptibility gene for type 1 diabetes mellitus by integration of functional analysis of recombinant chromosome with bioinformatics
| Project/Area Number |
17590929
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Osaka University |
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Principal Investigator |
FUJISAWA Tomomi Osaka University, Graduate School of Medicine, Assistant, 医学系研究科, 助手 (10324766)
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| Co-Investigator(Kenkyū-buntansha) |
IKEGAMI Hiroshi Osaka University, Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (20221062)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2005: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Type 1 diabetes mellitus / gene / bioinformatics / recombinant chromosome / type 1 diabetes mellitus / 疾患感受性 / モデル動物 |
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| Research Abstract |
Major histocompatibility complex (MHC)-linked chromosomal region confers strong genetic predisposition to type 1 diabetes mellitus both in human and NOD mouse, an animal model for type 1 diabetes. By investigating a congenic NOD.CTS-H-2 line whose chromosomal region encompassing the MHC is derived from the CTS mouse with the identical MHC class II to the NOD mouse, we have shown that the MHC-linked strong susceptibility is conferred by multiple genes and NOD has a susceptibility gene(s) outside class II region, termed Idd16. In the present project, we have clarified the following points ;
1 We established, by selective breeding, two subcongenic lines (NOD.CTS-H2R1 and NOD.CTS-H2R2) with new recombinant chromosomes.
2 The development of diabetes of the sub congenic line NOD.CTS-H2R1 was suppressed compared to that of the NOD-type littermates (control).
3 In contrast, as for the other subcongenic line NOD.CTS-H2R2, it's development of diabetes as well as the degree of insulitis were not significantly different from that of the NOD-type littermates (control).
4 Taken these data together, Iddl6 was further mapped to the region (1.62 + 0.24cM) adjacent to but distinct from the class II region, to the disease susceptibility.
5 Establishment of a new subcongenic NOD.CTS-H2 line with a shorter congenic interval facilitates further narrowing down of the localization of Idd16.
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Report
(3 results)
Research Products
(45 results)
